miR-122 inhibits metastasis and epithelial–mesenchymal transition of non-small-cell lung cancer cells

Abstract

miR-122 may function as a novel tumor suppressor. Expression of miR-122 could suppress the proliferation of multi-kinds of human cancer cell lines. In this work, expression of miR-122 via adenoviral vector in non-small-cell lung cancer (NSCLC) cells reduces the number of invasion and migration cells. miR-122 attenuates the epithelial–mesenchymal transition process, which mediates cancer cells metastasis in NSCLC cells A549 and H460. The mechanisms data reveals that miR-122 would disrupt the epithelial–mesenchymal transition process by downregulating PI3K/AKT activation via reducing endogenous expression of insulin-like growth factor 1 receptor. These data highlight the detailed roles and potential application of miR-122 in NSCLC cells.

<p>Up-Regulation of FSTL3, Regulated by lncRNA DSCAM-AS1/miR-122-5p Axis, Promotes Proliferation and Migration of Non-Small Cell Lung Cancer Cells</p>

“…27 In NSCLC, the expression of miR-221 and miR-10a were up-regulated, and their overexpression could accelerate the proliferation, migration and invasion of cancer cells. 28,29 Consistent with previous reports, 16,17 we confirmed that miR-122-5p was down-regulated in NSCLC tissues and involved in the progression of NSCLC as a tumor suppressor. In addition, we also found that there was a binding site between miR-122-5p and the 3ʹUTR of FSTL3, and the expression of FSTL3 and miR-122-5p was negatively correlated in NSCLC tissues.…”

“…15 Additionally, miR-122-5p is underexpressed in NSCLC, which can regulate the epithelialmesenchymal transition (EMT) of NSCLC cells. 16,17 This study explored the biological function of FSTL3 in NSCLC. We proved that FSTL3 expression was upregulated in NSCLC, and overexpression of FSTL3 could promote proliferation and metastasis of NSCLC, while knockdown of FSTL3 played an opposite role.…”

miR-122-5p as a novel biomarker for alpha-fetoprotein-producing gastric cancer

“…In fact, AFPGC was not necessarily hepatoid adenocarcinoma in this series, and two patients with aggressive development of liver metastasis were diagnosed morphologically as poorly differentiated adenocarcinoma and mucinous adenocarcinoma. Conversely, miR-122-5p was previously shown to function as a tumor suppressor and was reported to be downregulated in several cancer types such as hepatocellular carcinoma[ 24 ], non-small-cell lung cancer[ 25 ], gallbladder carcinoma[ 26 ], bladder cancer[ 27 ], and breast cancer[ 28 ]. In GC, the expression of miR-122-5p was reported to be lower in tumor tissue than the adjacent non-cancerous tissue.…”

Transcription factor activity of estrogen receptor &alpha; activation upon nonylphenol or bisphenol A treatment enhances the in vitro proliferation, invasion, and migration of neuroblastoma cells

“…The cells were incubated in the transwells at 37°C in 5% CO 2 for 4 hours (for migration) or 24 hours. 24 Finally, invading or migrating cells were fixed and stained with critical violet (0.5% diluted in 20% ethanol). The migration or invasion cells were harvested by glacial acetic acid and measured using a multifunctional microplate reader at 546 nm.…”