miR-101 inhibits glioma cell invasion via the downregulation of COX-2

Abstract

Glioma is the most common type of primary tumor of the central nervous system. The present study aimed to demonstrate the role of miR-101 and cyclooxygenase-2 (COX-2) in the initiation and development of glioma. The expression of miR-101 and COX-2 in normal and malignant human glial cells and tissues was determined by western blotting and quantitative polymerase chain reaction analysis. The role of miR-101 on COX-2 expression was evaluated by a dual-luciferase reporter assay. The effects of miR-101 and COX-2 in glioma cell proliferation and invasion was verified by CCK-8 test and Transwell assays, respectively. The present study demonstrated that miR-101 expression was downregulated while COX-2 was upregulated in glioma tissues and cells. Furthermore, transfection of miR-101 significantly downregulated COX-2 expression in both U373 and U87 glioma cells. In addition, further experiments revealed that overexpression of miR-101 resulted in significant inhibition of the in vitro proliferation and migration of glioma cells, and the in vivo growth of established tumors. Direct downregulation of COX-2 by transfection with corresponding small interfering RNA also inhibited the proliferation and invasion of glioma cells. These results indicate that downregulation of miR-101 is involved in the initiation and development of glioma via COX-2 upregulation.

Sphingosine‐1‐phosphate suppresses chondrosarcoma metastasis by upregulation of tissue inhibitor of metalloproteinase 3 through suppressing miR‐101 expression

“…Accumulating evidence revealed that miRNA regulate several cancer‐related processes during tumorigenesis through binding to the 3′UTR of the target genes (Nugent, ; Tsai et al ., ). Furthermore, miR‐101 was considered to regulate cell proliferation, angiogenesis, invasion, and migration in many types of tumors and serve as a tumor suppressor (Li et al ., ; Liu et al ., ; Ma et al ., ). In this study, we showed that extracellularly applied S1P or overexpressing SphK1 inhibit miR‐101 expression in human chondrosarcoma cells.…”

Ellagic Acid Inhibits Extracellular Acidity-Induced Invasiveness and Expression of COX1, COX2, Snail, Twist 1, and c-myc in Gastric Carcinoma Cells

“…Acidity was suggested to be associated with the induction of pro-inflammatory factors, such as TNF-α, IL-1β, and IL-6 [36], and COX2-dependent inflammatory responses [37]. Overexpression of COX2 has been suggested to be related to invasion in several types of cancer cells, including glioma and breast cancer cells [38,39], and inhibition of COX2 was shown to reduce lymphatic metastasis of a human gastric cancer cell line in xenografts [40]. Ellagic acid has been shown to exert anti-inflammatory activity in several disease models and decrease COX2-triggered exacerbation of inflammation [41,42].…”

Involvement of P2X7 Receptor in Proliferation and Migration of Human Glioma Cells

“…Malignant glioma is the most common malignant tumor of the central nervous system [ 1 ]. The invasive performance and easy transformation from lower grades to higher grades were the main causes of the poor prognosis of glioma [ 2 , 3 ].…”