MiR-1-3p Suppresses the Proliferation, Invasion and Migration of Bladder Cancer Cells by Up-Regulating SFRP1 Expression

Abstract

Key WordsMiR-1-3p • SFRP1 • bladder cancer Abstract Background: Bladder cancer is of compelling morbidity and mortality due to its high recurrence rate. Little development has been made in the last decades in the therapy methods. Thus, the mechanism of its growth and invasiveness involving novel molecular targets are needed. Objective: Our research objective is to confirm the hypothesis that miR-1-3p suppresses the proliferation, invasion and migration of bladder cancer cells. Methods: The expression levels of miR-1-3p and SFRP1 were evaluated using RT-qPCR in bladder cancer tissues and cells as well as in normal tissues and cells. J82 cell lines were selected as experiment subjects due to their low expression levels of miR-1-3p. Plasmids carrying miR-1-3p mimics, miR-1-3p inhibitors and SFRP1 were transfected into the J82 cell lines. Subsequently, the protein expression of SFRP1 was detected using Western Blot analysis, and cell proliferation, apoptosis, invasion and migration ability was measured using MTT, the flow cytometry, the Transwell test and wound healing assays, respectively. Results: Bladder cancer tissues and cells exhibited significant decrease in the expression of miR-1-3p and SFRP1 compared to normal tissues and cells, and human bladder cancer cell line J82 exhibited the most significant decrease in these expressions (P < 0.05). MiR-1-3p up-regulates SFRP1 expression in bladder cancer cells, and the over-expression of miR-1-3p can suppress the proliferation, invasion and migration ability of bladder cancer cells. This mechanism is similar to the effect of SFRP1 over-expression on bladder cancer cells. Conclusion: MiR-1-3p suppresses the proliferation, invasion and migration of bladder cancer cells by up-regulating SFRP1 expression.

miR-1-3p Contributes to Cell Proliferation and Invasion by Targeting Glutaminase in Bladder Cancer Cells

“…in tumorigenesis are yet to be elucidated. In the present study, we revealed that the basic expression levels of miR-1-3p in bladder cancer tissues and cell lines were significantly downregulated as compared to the paired adjacent non-tumor bladder urothelial tissues and the non-tumor urothelial cell line, respectively, which was consistent with the previous study [18]. In addition, our findings indicated that miR-1-3p functions as tumor suppressors in bladder cancer for its effects on the inhibition of proliferation, migration and invasion.…”

“…miR-1 can inhibit the malignant transformation of bladder cancer cells by targeted regulation of TAGLN2 [17]. Shang et al demonstrated that miR-1-3p inhibited bladder cancer cells with respect to proliferation, invasion and migration by up-regulating the expression of SFRP1 [18]. Accumulating evidence suggested that miRNAs have a unique ability to regulate multiple protein-coding genes via direct interaction with 3'-UTR of the target mRNAs.…”

CircRNA-Cdr1as Exerts Anti-Oncogenic Functions in Bladder Cancer by Sponging MicroRNA-135a

“…NcRNAs played important roles in bladder cancer [3]. For example, microRNAs may regulate the proliferation, migration, invasion [4, 5] and the chemo-sensitivity [6] of bladder cancer. Long Non-coding RNAs also showed huge functions in bladder cancer，including tumorigenesis, growth [7], apoptosis [8], metastasis [9] and so on.…”

Prognostic Implication of Urothelial Stem Cell Markers Differs According to Primary Tumour Location in Non-Muscle-Invasive Bladder Cancer

“…Increasing evidence suggests stem cell (SC) markers might be useful in predicting the clinical prognosis of human cancers, as many marker gene products found in stem cells are shared with the cancer stem cell (CSC) population, which is responsible for key elements of cancer progression, metastasis, and recurrence [5,6]. In addition, SC markers have been used for the identification and isolation of putative SCs or CSCs.…”