MIPs–SERS Sensor Based on Ag NPs Film for Selective Detection of Enrofloxacin in Food

Abstract

The quinolone antibiotics represented by enrofloxacin (ENRO) are harmful to the ecological environment and human health due to illegal excessive use, resulting in increasing food residues and ENRO levels in the environment. To this end, we developed a MIPs–SERS method using surface-enhanced Raman spectroscopy (SERS) and molecularly imprinted polymers (MIPs) to detect ENRO in food matrices. Firstly, a layer of silver nanoparticles (Ag NPs) with the best SERS effect was synthesized on the surface of copper rods as the enhancing material by in situ reductions, and then MIPs targeting ENRO were prepared by the native polymerization reaction, and the MIPs containing template molecules wrapped on the surface of silver nanoparticle films (Ag NPs–MIPs) were obtained. Our results showed that the Ag NPs–MIPs could specifically identify ENRO from the complex environment. The minimum detection limit for ENRO was 0.25 ng/mL, and the characteristic peak intensity of ENRO was linearly correlated to the concentration with a linear range of 0.001~0.1 μg/mL. The experimental results showed that in comparison to other detection methods, the rapid detection of ENRO in food matrices using Ag NPs–MIPs as the substrate is reliable and offers a cost-effective, time-saving, highly selective, and sensitive method for detecting ENRO residues in real food samples.

Molecularly Imprinted Polymer-Based Sensors for the Detection of Skeletal- and Cardiac-Muscle-Related Analytes

“…Minced pork was spiked with different concentrations (0.1, 0.05, and 0.02 μg/mL) of ENRO, the supernatants extracted were placed on the sensor for 120 min, and three measurements for each concentration were performed. The recovery rates ranged between 90.61 and 92.72% with an RSD of 2.42–5.16%, and these results were consistent with those obtained by HPLC evaluation [ 136 ]. The combination of SERS and MIP techniques is interesting because SERS has a high sensitivity, whereas MIP also has a high selectivity.…”

The Preparation of Silver and Gold Nanoparticles in Hyaluronic Acid and the Influence of Low-Pressure Plasma Treatment on Their Physicochemical and Microbiological Properties

“…Consequently, silver nanoparticles (AgNPs) are extensively employed in medicine for various purposes such as dressings, bandages, antimicrobial creams, and disinfectants [6]. According to the literature, antimicrobial nanomaterials are often referred to as 'nanoantibiotics' and are regarded as one of the most promising methods for preventing or controlling the growth and spread of microbes and infections [7][8][9][10]. Nanosilver is highly chemically stable and resistant to corrosion, which allows it to maintain its properties even in highly acidic or alkaline environments [11].…”