Minutaside A, new α‐amylase inhibitor flavonol glucoside from Tagetes minuta: Antidiabetic, antioxidant, and molecular modeling studies

Abstract

. Their structures were established by multiple spectroscopic methods in addition to HRESIMS (high-resolution electrospray ionisation mass spectra) and comparison with literature data. The antioxidant and anti-diabetic activities of the isolated flavonoids were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and a-amylase inhibition assays. Compounds 1 and 5 showed significant antioxidant activity (84.1 and 83.0% at a 20 mM dose, respectively). Compounds 1, 4, and 5 exhibited strong aamylase inhibitory activity compared with acarbose (a reference a-amylase inhibitor). However, 2 and 3 showed moderate activity. Molecular modeling studies of 1-5 that included docking, flexible alignment, and surface mapping were performed to evaluate their recognition profile aamylase receptor. In docking simulations, 5 displayed a binding mode similar to that of acarbose in the active site of a-amylase enzyme.

α‐Amylase inhibition of xanthones fromGarcinia mangostanapericarps and their possible use for the treatment of diabetes with molecular docking studies

“…The assay was carried out using α‐amylase by EnzCheck ® Ultra Amylase Assay Kit (E33651) as previously outlined (Ibrahim, Mohamed, Abdel‐Latif, et al, ; Ibrahim, Mohamed, Zayed, et al, ; Ibrahim, Al‐Ahdal, et al, ; Mohamed, ).…”

Garcixanthone D, a New Xanthone, and Other Xanthone Derivatives From Garcinia mangostana Pericarps: Their α‐Amylase Inhibitory Potential and Molecular Docking Studies

“…EnzCheck Ultra Amylase Assay Kit (E33651) (Thermo Fisher Scientific, Paisley, UK) was used to assess the α‐amylase inhibitory activity as previously reported . Acarbose was used as standard a‐amylase inhibitor (Kohlpharma, GmbH, Koln, Germany).…”

“…α‐Amylase is a calcium metallo‐enzyme that hydrolyses the α‐bonds of α‐linked polysaccharides, such as glycogen and starch, producing maltose and glucose . It is found in normal urine, saliva, in human serum and is also produced by different species, including plants, animals, and microorganisms .…”

Thiotagetin A, a new cytotoxic thiophene from Tagetes minuta

“…It has been used as a remedy for several medical aliments such as colds, respiratory inflammations, stomach problems, catarrh, wounds, cuts, calluses and bunions (Gillij et al 2008;Rahimi et al 2010;Maity et al 2011). Moreover, it showed a wide range of biological properties, including α-amylase inhibitor, antimicrobial, anti-spasmodic, anti-parasitic, anti-septic, insecticidal, sedative, anti-inflammatory and acaricidal (Tereschuk et al 1997;Shahzadi et al 2010;Andreotti et al 2013;Ibrahim et al 2015). Previous chemical investigations of T. minuta growing in Saudi Arabia revealed the presence of thiophenes and flavonoids (Al-Musayeib et al 2014;Ibrahim et al 2015).…”

“…Moreover, it showed a wide range of biological properties, including α-amylase inhibitor, antimicrobial, anti-spasmodic, anti-parasitic, anti-septic, insecticidal, sedative, anti-inflammatory and acaricidal (Tereschuk et al 1997;Shahzadi et al 2010;Andreotti et al 2013;Ibrahim et al 2015). Previous chemical investigations of T. minuta growing in Saudi Arabia revealed the presence of thiophenes and flavonoids (Al-Musayeib et al 2014;Ibrahim et al 2015). In the present study, a new thiophene derivative: thiotagetin A (3), along with β-sitosterol (1) and stigmasterol (2) were isolated from the aerial parts of T. minuta ( Figure 1).…”