Minoxidil activates β-catenin pathway in human dermal papilla cells: A possible explanation for its anagen prolongation effect

Kwack, Mi Hee; Kang, Byungjun; Kim, Moon Kyu; Kim, Jung Chul; Sung, Young Kwan

doi:10.1016/j.jdermsci.2011.01.013

Cited by 105 publications

(112 citation statements)

References 27 publications

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“…Consequently, minoxidil stimulated cell proliferation and prevented cell death. Kwack et al 27 found that the phosphorylation of GSK3β, PKA, and PKB and expression of Axin2, Lef-1, and EP2 were accelerated by minoxidil in HFDPC. Lachgar et al 28 demonstrated that minoxidil in the range of 0.2 to 12 µM raised VEGF mRNA expression and protein in HFDPC.…”

Section: Discussionmentioning

confidence: 99%

Visible-to-Near IR Quantum Dot–Based Hypermulticolor High-Content Screening of Herbal Medicines for the Efficacy Monitoring of Hair Growth Promotion and Hair Loss Inhibition

Kim

Lim

Lee³

et al. 2013

SLAS Discovery

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There is a growing interest in alopecia prevention strategies, as the number of alopecia patients is increasing. We examine the efficacy of herbal medicine for hair growth promotion/hair loss inhibition in two cell lines via Western blot and highcontent screening (HCS). Nine herbal extracts were obtained from three different herbal medicine mixtures using 3 different extraction methods. Five target proteins-IGF-1 (insulin-like growth factor-1), TGF-β2 (transforming growth factor-β2), VEGF (vascular endothelial growth factor), DKK-1 (Dickkopf-1), and Wnt5α-were observed for the assessment of hair growth promotion/hair loss inhibition efficacy. The efficacies of nine extracts were compared with minoxidil as control. Efficacy was defined as a rise in the expression levels of IGF-1, VEGF, and Wnt5α but a decrease in DKK-1 and TGF-β2. Intracellular concurrent imaging of these proteins was successfully achieved using HCS, employing visible-to-near infrared probing based on quantum-antibody conjugates and hypermulticolor imaging.

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Section: Discussionmentioning

confidence: 99%

Visible-to-Near IR Quantum Dot–Based Hypermulticolor High-Content Screening of Herbal Medicines for the Efficacy Monitoring of Hair Growth Promotion and Hair Loss Inhibition

Kim

Lim

Lee³

et al. 2013

SLAS Discovery

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“…13,22) To confirm the hair growth promoting effect of apo-9′-fucoxanthinone in relation to the Wnt/β-catenin pathway, we examined the change in the level of phospho-GSK-3β, GSK-3β, phospho(ser552)-β-catenin and phospho(ser675)-β-catenin following apo-9′-fucoxanthinone treatment for 72 h in DPCs. Treatment with apo-9′-fucoxanthinone resulted in an increase in the level of phospho(ser9)-GSK-3β and phospho(ser552)-β-catenin compared to control cells (Fig.…”

Section: Effect Of Apo-9′-fucoxanthinone On the 5α-reductase Activitymentioning

confidence: 99%

“…The action mechanisms of minoxidil on hair growth include the increase of ATP-sensitive K + -channel opening, the upregulation of vascular endothelial growth factor (VEGF) and the activation of the β-catenin pathway in dermal papilla cells (DPCs). [11][12][13] However, because of unwanted side effects and transient action, dissatisfied responses to finasteride and minoxidil in alopecia have been reported. 14,15) Therefore, the demand for new therapies for alopecia is on the rise.…”

mentioning

confidence: 99%

“…18) Indeed, minoxidil extends the anagen (growth) phase of the hair cycle via the activation of Wnt/β-catenin in DPCs. 13) The expression of Wnt/β-catenin proteins has been observed in stem cells, 19) cancer cells 20) and hair follicle cells 21) and several studies suggest that the activation of β-catenin, the main component of Wnt/β-catenin, regulates stem cell function, 19) cell survival 20) and proliferation. 22) The level of β-catenin is regulated by the disruption complex, including axin, adenomatous polyposis coli, casein kinase I and glycogen synthase kinase-3β (GSK-3β).…”

mentioning

confidence: 99%

“…GSK-3β inhibitor increases hair growth resulting from the proliferation of DPCs by increasing the β-catenin level. 13,23) VEGF is an important regulator in angiogenesis and tumorigenesis. [24][25][26] The effect of VEGF is mediated by the binding of VEGF receptor-2 (VEGF-R2) and recent studies showed that VEGF-mediated proliferation effects have been observed in DPCs and outer root sheaths (ORS).…”

mentioning

confidence: 99%

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Promotion Effect of Apo-9′-fucoxanthinone from <i>Sargassum muticum</i> on Hair Growth <i>via</i> the Activation of Wnt/β-Catenin and VEGF-R2

Kang

Yoo

Hyun

et al. 2016

Biological & Pharmaceutical Bulletin

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This study was conducted to evaluate the effects of Sargassum muticum extract and apo-9′-fucoxanthinone, a principal component of S. muticum, on hair growth. When rat vibrissa follicles were treated with S. muticum extract for 21 d, the hair-fiber lengths for the vibrissa follicles increased significantly. Treatment with the S. muticum extract and the EtOAc fraction of the S. muticum extract markedly increased the proliferation of dermal papilla cells (DPCs) and decreased the 5α-reductase activity. In addition, the EtOAc fraction of the S. muticum extract significantly promoted anagen initiation in C57BL/6 mice. Especially, apo-9′-fucoxanthinone, an active constituent from the S. muticum extract, caused an increase in DPC proliferation and a decrease in 5α-reductase activity. To elucidate the molecular mechanisms of apo-9′-fucoxanthinone on the proliferation of DPCs, we examined the level of various signaling proteins. Apo-9′-fucoxanthinone increased the level of vascular endothelial growth factor receptor-2 (VEGF-R2), Wnt/β-catenin signaling proteins such as phospho(ser9)-glycogen synthase kinase-3β (GSK-3β) and phospho(ser552)-β-catenin, whereas apo-9′-fucoxanthinone did not affect the transforming growth factor-β (TGF-β) signaling proteins such as Smad2/3. These results suggest that apo-9′-fucoxanthinone from S. muticum could have the potential for hair growth with DPC proliferation via the activation of Wnt/β-catenin signaling and the VEGF-R2 pathway.

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A Bio‐Functional Wnt3a Gradient Microarray for Cell Niche Studies

Wang

Feng

et al. 2023

Adv Funct Materials

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The Wingless/Integrated (Wnt) signaling is a central player in regulating multiple cellular events ranging from developmental process, adult homeostasis to tumorigenesis. In native microenvironments, Wnt signals often function through the concentration gradients between the secreting site and the targeting site. Hence, presenting Wnt molecules in a concentration gradient in vitro in a controllable manner recapitulates the native Wnt niche. Nevertheless, most in vitro Wnt niche models, where the ligands are homogeneously immobilized on material surface, fail in reconstituting the concentration gradient of Wnt as that in vivo. Herein, using a previously established Multiphoton Microfabrication and Micropatterning (MMM) technology, a concentration gradient of Wnt3a is immobilized on a protein substrate via neutravidin‐biotin interactions, in a quantitatively and spatially controllable manner. Such Wnt3a gradient is bioactive as it triggers a dose‐ and time‐dependent upregulation of β‐catenin and cell proliferation, in model mouse L cells. Most importantly, human hair dermal papilla cells (HHDPCs) cultured in a cell niche with Wnt3a gradient proliferate in a Wnt3a‐gradient‐dependent manner and exhibit a Wnt3a‐gradient‐dependent hair‐inductive phenotype including Wnt/β‐catenin signaling activation and alkaline phosphatase (ALP) expression. This work contributes to the future development of a hair follicle inductivity biochip for screening of drugs and therapeutics on hair regeneration.

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Minoxidil activates β-catenin pathway in human dermal papilla cells: A possible explanation for its anagen prolongation effect

Cited by 105 publications

References 27 publications

Visible-to-Near IR Quantum Dot–Based Hypermulticolor High-Content Screening of Herbal Medicines for the Efficacy Monitoring of Hair Growth Promotion and Hair Loss Inhibition

Visible-to-Near IR Quantum Dot–Based Hypermulticolor High-Content Screening of Herbal Medicines for the Efficacy Monitoring of Hair Growth Promotion and Hair Loss Inhibition

Promotion Effect of Apo-9′-fucoxanthinone from <i>Sargassum muticum</i> on Hair Growth <i>via</i> the Activation of Wnt/β-Catenin and VEGF-R2

A Bio‐Functional Wnt3a Gradient Microarray for Cell Niche Studies

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