The current study investigates whether eye color provides a marker of Agreeableness in North Europeans. Extrapolating from Frost's (2006) research uncovering an unusually diverse range of hair and eye color in northern Europe, we tested the hypothesis that light eyed individuals of North European descent would be less agreeable (a personality marker for competitiveness) when compared to their dark eyed counterparts, whereas there would be no such effect for people of European descent in general. The hypothesis was tested in Australia to provide consistent environmental conditions for both groups of people. Results support the hypothesis. Implications and conclusions are discussed.Since the influential book on eye color by Morgan Worthy (1999), a small number of researchers have been interested in whether eye color provides an indication of personality and associated behavior. Eye color is determined by the number and size of melanin particles in the layers of the iris. More melanin therefore makes the eyes appear darker (Sturm and Frudakis 2004). The lightest shade is blue through gray, green, hazel and brown to black, the darkest shade. Eye color has been shown to be related to alcohol use (Bassett and Dabbs 2001), response styles (Worthy 1999), non-verbal communication and sociability (Gary and Glover 1976). Whilst the proposal of a relationship between the amount of melanin in the iris and behavior seems unexpected, there are some good theoretical reasons for thinking that such a relationship may exist.