Minor tumor infiltrating B cells opened a door to reveal and eliminate cancer initiating cells in metastatic melanomas

Kotlan, Beatrix; Liszkay, Gabriella; Naszados, Gyorgy; Olasz, Judit; Horvath, Szabolcs; Plótár, Vanda; Szollar, Andras; Vamosi, Istvan Nagy; Sávolt, Ákos; Tóth, László; Csuka, Orsolya; Gödény, Mária; Kásler, Miklós; Marincola, Francesco M.

doi:10.1186/2051-1426-2-s1-p4

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“…Important previous and recent findings evidence the fact that the detection of various glycosphingolipid-specific human antibodies have diagnostic value [ 29 ] and should be harnessed for new therapeutic strategies. All this is in line with our recent findings on a new technology to reveal cancer stem cells [ 16 , 30 ].…”

Section: Discussionsupporting

confidence: 89%

“…B lymphocites accumulated in the tumor microenvironment, the so called tumor infiltrating B cells (TIL-B) that can be found in breast cancers and other solid tumors [ 14 , 15 ], can be analysed in terms of their antibody repertoire. These pioneering results could be corroborated in malignant melanomas [ 16 ]. We describe here a complex protocol by harnessing immune B cells from the tumor microenvironment to reveal tumorassociated antigens (TAA).…”

Section: Introductionmentioning

confidence: 80%

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Challenging tumour immunological techniques that help to track cancer stem cells in malignant melanomas and other solid tumours

Kotlan

Plótár

Éles

et al. 2018

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Aim of the studyThe arsenal of questions and answers about the minor cancer initiating cancer stem cell (CSC) population put responsible for cancer invasiveness and metastases, has left with an unsolved puzzle. Specific aims of a complex project were partly focused on revealing new biomarkers of cancer. We designed and set up novel techniques to facilitate the detection of cancerous cells.Materials and methodsAs a novel approach, we investigated B cells infiltrating breast carcinomas and melanomas (TIL-B) in terms of their tumour antigen binding potential. By developing the TIL-B phage display technology we provide here a new technology for the specific detection of highly tumour-associated antigens. Single chain Fv (scFv) antibody fragment phage ELISA, immunofluorescence (IF) FACS analysis, chamber slide technique with IF confocal laser microscopy and immunohistochemistry (IHC) in paraffin-embedded tissue sections were set up and standardized.ResultsWe showed strong tumour-associated disialylated glycosphingolipid expression levels on various cancer cells using scFv antibody fragments, generated previously by uniquely invasive breast carcinoma TIL-B phage display library technology.ConclusionsWe report herein a novel strategy to obtain antibody fragments of human origin that recognise tumour-associated ganglioside antigens. Our investigations have the power to detect privileged molecules in cancer progression, invasiveness, and metastases. The technical achievements of this study are being harnessed for early diagnostics and effective cancer therapeutics.

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Section: Discussionsupporting

confidence: 89%