Minor flavonoids from licorice

Coumarins from Campylotropis hirtella (FRANCH.) SCHINDL. and Their Inhibitory Activity on Prostate Specific Antigen Secreted from LNCaP Cells

“…The UV spectroscopic data (l max 259, 352 nm) of this compound were similar to those of licofuranocoumarin and licopyranocoumarin, 6,7) suggesting a 3-arylcoumarin structure for 1. The was assigned as C-5.…”

“…Cell Culture and Test Compounds Preparation LNCaP cells were maintained at 37°C in RPMI 1640 (Gibco, U.S.A.) supplemented with 10% heat-inactivated FBS, 100 U/ml penicillin-streptomycin (Hyclone, U.S.A.). The fractions and isolated compounds (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13) were dissolved in DMSO to obtain the desired concentrations.…”

Licorice as a Resource for Pharmacologically Active Phenolic Substances: Antioxidant and Antimicrobial Effects

“…Thus, centrifugal partition chromatography (CPC) and droplet countercurrent chromatography (DCCC), which were devised as efective methods for CCD, in addition to simple CCD using separatory funnels, were applied to purify the licorice phenolics in our studies. The solvent system chloroform-methanol-water (7:13:8, by volume) was primarily used for the separation of licorice phenolics derived from Glycyrrhiza inlata [9,10] and those derived from G. uralensis [11][12][13][14][15][16] in these CCD processes. Combinations of column chromatography on a silica gel, ODS-gel, and/or polystyrene gel (MCI-gel CHP-20P) with CCD also aforded satisfactory separation [17,18].…”

“…Electron impact mass spectrometry (EI-MS) is a useful method for obtaining structural information using fragment ions [16]. On the other hand, fast-atom bombardment (FAB) and electrospray ionization mass spectrometry (ESI-MS) are applicable to the ionization of phenolics, including phenolic glycosides.…”

“…Notably, the high-resolution FAB and ESI-MS have been used to determine their molecular formulae [17]. Ultraviolet-visible (UV-Vis) spectroscopy was useful for discriminating between phenolic skeletons even if the 1 H NMR spectra were quite similar to each other, as was the case for 3-arylcoumarins and the corresponding isolavones [16]. Electronic circular dichroism (ECD) spectroscopy was efective not only for identifying the coniguration of asymmetric carbons (e.g., those in lavanones, isolavans, and isolavanones [9,15,17]) in the lavonoid skeletons but also for explaining the spatial relationship between the chromophores in acylated lavonoid glycoside molecules [17].…”