Minireview: Functions of the cumulus oophorus during oocyte maturation, ovulation, and fertilization

Tanghe, Sofie; Soom, Ann Van; Nauwynck, Hans; Coryn, M.; Kruif, Aart de

doi:10.1002/mrd.10102

Cited by 414 publications

(297 citation statements)

References 143 publications

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“…These results differ from those reported after homologous IVF, which have shown that the cumulus oophorus plays a role in blocking polyspermy and increase embryo formation since they are able to transfer nutrients and regulatory signals to the oocyte [10,68].…”

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confidence: 75%

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Heterologous murine and bovine IVF using bottlenose dolphin (Tursiops truncatus) spermatozoa

et al. 2015

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20Assisted reproductive technologies (ARTs) are of great importance for increasing the genetic diversity in captive animals. The use of bovine or murine oocytes in heterologous IVF provide advantages compared to homologous IVF in non-domestic animals, such as the accessibility to oocytes and the availability of well-developed in vitro maturation systems. The aim of this study was to determine the heterologous IVF 25 parameters using cryopreserved dolphin spermatozoa and zona-intact bovine or murine oocytes, as well as to examine the nuclear chromatin status of the dolphin spermatozoa.All the processes involved in the fertilization including embryo cleavage were observed by confocal microscopy and hybrid embryo formation was confirmed by PCR.Heterologous bovine IVF showed no polyspermy, lower percentages of pronuclear 30 formation and a lower cleavage rate compared to homologous IVF group (34.8 vs.89.3%). Heterologous murine IVF showed a lower cleavage rate than homologous IVF (9.6 vs. 77.1%). With respect to dolphin sperm chromatin, it was more stable, i.e. more resistant to EDTA-SDS decondensation than the bovine sperm chromatin. This study revealed the stability of the dolphin sperm chromatin and the ability of the dolphin 35 spermatozoa to penetrate zona-intact bovine and murine oocytes, leading to hybrid embryo formation.

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contrasting

confidence: 75%

“…On the 60 other hand, the ZP removal prevents obtaining information on basic sperm functions, such as the sperm-zona interaction and penetration. In addition, the cumulus oophorus cells are removed with the ZP, which can alter oocyte homeostasis and influence parameters such as polyspermy, pronuclear formation and embryo cleavage [10,11].…”

Section: Introductionmentioning

confidence: 99%

Heterologous murine and bovine IVF using bottlenose dolphin (Tursiops truncatus) spermatozoa

et al. 2015

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“…The interaction of the oocyte and the CCs occurs at gap junctions [41], through which nutrients and maturationenabling factors are provided and where the exchange of metabolites and ions between the oocyte and the CCs is facilitated [13]. Therefore, reciprocal and optimal exchange between the oocyte and the CCs is essential for the acquisition of oocyte competence.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, reciprocal and optimal exchange between the oocyte and the CCs is essential for the acquisition of oocyte competence. It has been hypothesized that studying these cells can elucidate the physiological importance of these processes and may lead to optimization of IVF [41]; the lipid composition of the CCs may reflect and determine oocyte maturation, fertilization and early embryo development potential.…”

Section: Introductionmentioning

confidence: 99%

The follicular microenviroment as a predictor of pregnancy: MALDI-TOF MS lipid profile in cumulus cells

Montani

Cordeiro

Regiani

et al. 2012

J Assist Reprod Genet

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Purpose This research proposed to study the changes in lipid composition in cumulus cells (CCs) from women who achieved pregnancy compared with women who did not, after in vitro fertilization treatment. This approach has the potential to provide novel information on the lipid metabolism of the CCs and as an additional method to predict pregnancy. Method Fifty-four samples from couples with tubal and male factor infertility and where the female partner was age 35 or younger were divided in two groups according to their level of hCG 14 days after embryo transfer as follows: (1) 23 samples in pregnant group and (2) 31 samples in non-pregnant group. Lipid extraction was performed by the Bligh-Dyer protocol, and lipid profiles were obtained by MALDI-TOF MS. Mass spectra data were processed with MassLynx, and statistical analysis was performed using MarkerLynx extended statistic. OPLS-DA model was built. Results S-plot Analysis revealed three ions as potential markers in the pregnant group, and five ions in the nonpregnant group. These ions were identified in the human metabolome database (HMDB) as phosphatidylcholine in the pregnant group and as phosphatidylethanolamine, phosphatidylserine and phosphatidylinositol species in the non-pregnant group. These lipids might be involved in cell proliferation and differentiation, apoptosis and GAP junction regulation. Conclusion We conclude that MALDI-TOF MS can be used as an informative and fast analytical strategy to obtain and study the lipid profile of cumulus cells and can potentially be used as a supporting tool to predict pregnancy based on the metabolic state of the CCs.

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“…The multiple roles of the cumulus oophorus have been reviewed [15,28]. These cells may be an obstacle to the penetration of cryoprotectants [29] or the gap junctions can be altered by cryopreservation.…”

Section: Cumulus Cell Investmentmentioning

confidence: 99%

Cryopreservation of bovine oocytes: Current status and recent developments

Massip

2003

Reprod. Nutr. Dev.

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-The cryopreservation of oocytes of most animal species remains a challenge due to their complex structure. Equilibrium freezing is not satisfactory because oocytes seem to be damaged by exposure for several minutes to temperatures near 0 ºC. Therefore, cryopreservation of oocytes by vitrification -especially the use of very high cooling rates of oocytes suspended in extremely small volumes of various cryoprotective additive modifications -seems the most appropriate method. oocyte / bovine / cryopreservation

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Minireview: Functions of the cumulus oophorus during oocyte maturation, ovulation, and fertilization

Cited by 414 publications

References 143 publications

Heterologous murine and bovine IVF using bottlenose dolphin (Tursiops truncatus) spermatozoa

Heterologous murine and bovine IVF using bottlenose dolphin (Tursiops truncatus) spermatozoa

The follicular microenviroment as a predictor of pregnancy: MALDI-TOF MS lipid profile in cumulus cells

Cryopreservation of bovine oocytes: Current status and recent developments

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