Mining sequences with similarity to XTH genes in the Solanum tuberosum L. transcriptome: introductory step for identifying homologous XTH genes

Hidvégi, Norbert; Gulyás, Andrea; Dobránszki, Judit; Silva, Jaime A. Teixeira da

doi:10.1080/15592324.2020.1797294

Cited by 10 publications

(5 citation statements)

References 36 publications

(66 reference statements)

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“…(2022) used morphological, biochemical, and molecular markers from Withania somnifera to assess the 25 accessions of Indian ginseng, and concluded that these markers could be used to select superior ginseng genotypes. In addition, XTH genes were also reported to be involved in other abiotic stress, such as salt, heat and cold stress ( Han et al., 2017 ; Hidvégi et al., 2020 ; De Caroli et al., 2021 ). Hence, we suggest that LcXTH genes play roles in drought resistance and other abiotic stresses.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification of XTH genes in Liriodendron chinense and functional characterization of LcXTH21

Zong

et al. 2022

Front. Plant Sci.

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Liriodendron chinense is a relic tree species of the family Magnoliaceae with multiple uses in timber production, landscape decoration, and afforestation. L. chinense often experiences drought stress in arid areas. However, the molecular basis underlying the drought response of L. chinense remains unclear. Many studies have reported that the xyloglucan endotransglucosylase/hydrolase (XTH) family plays an important role in drought stress resistance. Hereby, to explore the drought resistance mechanism of L. chinense, we identify XTH genes on a genome-wide scale in L. chinense. A total of 27 XTH genes were identified in L. chinense, and these genes were classified into three subfamilies. Drought treatment and RT-qPCR analysis revealed that six LcXTH genes significantly responded to drought stress, especially LcXTH21. Hence, we cloned the LcXTH21 gene and overexpressed it in tobacco via gene transfer to analyze its function. The roots of transgenic plants were more developed than those of wild-type plants under different polyethylene glycol (PEG) concentration, and further RT-qPCR analysis showed that LcXTH21 highly expressed in root compared to aboveground organs, indicating that LcXTH21 may play a role in drought resistance through promoting root development. The results of this study provide new insights into the roles of LcXTH genes in the drought stress response. Our findings will also aid future studies of the molecular mechanisms by which LcXTH genes contribute to the drought response.

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Section: Discussionmentioning

confidence: 99%

Genome-wide identification of XTH genes in Liriodendron chinense and functional characterization of LcXTH21

Zong

et al. 2022

Front. Plant Sci.

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“…Previous studies showed that there was a good correlation between the activity of XET and the growth rate and the elongation of cell wall [ 15 , 52 – 54 ]. Vissenberg et al (2005) reported that the activity of XET was high near the starting point of root hair, and it was believed that XET activity was all high in elongation region of roots in various vascular plants [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Identification and response analysis of xyloglucan endotransglycosylase/hydrolases (XTH) family to fluoride and aluminum treatment in Camellia sinensis

Cui

Xing

et al. 2021

BMC Genomics

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Background Xyloglucan endotransglycosylase/hydrolases (XTH) can disrupt and reconnect the xyloglucan chains, modify the cellulose-xyloglucan complex structure in the cell wall to reconstruct the cell wall. Previous studies have reported that XTH plays a key role in the aluminum (Al) tolerance of tea plants (Camellia sinensis), which is a typical plant that accumulates Al and fluoride (F), but its role in F resistance has not been reported. Results Here, 14 CsXTH genes were identified from C. sinensis and named as CsXTH1–14. The phylogenetic analysis revealed that CsXTH members were divided into 3 subclasses, and conserved motif analysis showed that all these members included catalytic active region. Furthermore, the expressions of all CsXTH genes showed tissue-specific and were regulated by Al3+ and F− treatments. CsXTH1, CsXTH4, CsXTH6–8 and CsXTH11–14 were up-regulated under Al3+ treatments; CsXTH1–10 and CsXTH12–14 responded to different concentrations of F− treatments. The content of xyloglucan oligosaccharide determined by immunofluorescence labeling increased to the highest level at low concentrations of Al3+ or F− treatments (0.4 mM Al3+ or 8 mg/L F−), accompanying by the activity of XET (Xyloglucan endotransglucosylase) peaked. Conclusion In conclusion, CsXTH activities were regulated by Al or F via controlling the expressions of CsXTH genes and the content of xyloglucan oligosaccharide in C. sinensis roots was affected by Al or F, which might finally influence the elongation of roots and the growth of plants.

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“…Other previous studies have reported downregulated expression of At XTH15 in Arabidopsis plants with rosette leaves manually folded for 30 minutes [43], suggesting that At XTH15 is a molecule whose expression is downregulated not only under drought but also under stresses such as mechanical stresses. On the other hand, it has been reported that the expression level of At XTH15 increases upon ultrasonic (US) treatment [44], and thus, it is necessary to investigate the relationship with various stress response mechanisms.…”

Section: Xyloglucan Endotransglucosylase/hydrolase (Xth) Familymentioning

confidence: 99%

Transcriptomic regulation of potato in response to drought stress

Kawamoto,

Masutomi,

Matsumoto

et al. 2023

Preprint

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Limited crop production due to lower rainfall has a major impact on the supply and demand of food for the human population. In potato (Solanum tuberosum L.), one of the major crops, there is also concern about a lack of production due to drought stress. Especially the cultivar "Toyoshiro" suitable for processing, has significant reduction in drought yield. Therefore, it is necessary to understand the mechanism of gene expression changes that occur in potato "Toyoshiro" plants and tubers during drought. Seed potatoes were split in half and one was used as a control plant that was watered daily, and the other was used as a drought-stressed plant that was watered off to mimic the weather conditions of the Tokachi-Obihiro region in 2021. These tubers were harvested at week 14 and the transcriptome was analyzed. The stressed plants showed 423 downregulated genes and 197 upregulated genes compared to the control. Factors related to phytosterol metabolism, cell wall modification, and heat stress response were detected among the genes whose expression changed. Moreover, the expression of "Abscisic acid and environmental stress-inducible protein TAS14 like (TAS14)," a molecule reported to be upregulated in several cultivars under drought stress, was also upregulated, and the localization of this molecule in the nucleus and plasma membrane was confirmed in an mCherry-tagged TAS14 mutant line. These results contribute to understanding the survival strategy system of potatoes in response to drought stress.

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Mining sequences with similarity to XTH genes in the Solanum tuberosum L. transcriptome: introductory step for identifying homologous XTH genes

Cited by 10 publications

References 36 publications

Genome-wide identification of XTH genes in Liriodendron chinense and functional characterization of LcXTH21

Genome-wide identification of XTH genes in Liriodendron chinense and functional characterization of LcXTH21

Identification and response analysis of xyloglucan endotransglycosylase/hydrolases (XTH) family to fluoride and aluminum treatment in Camellia sinensis

Transcriptomic regulation of potato in response to drought stress

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