Mining Lactonase Gene from Aflatoxin B<sub>1</sub>-Degrading Strain <i>Bacillus megaterium</i> and Degrading Properties of the Recombinant Enzyme

Cheng, Sizhong; Wu, Tian; Zhang, Hongxin; Sun, Zhongke; Mwabulili, Fred; Xie, Yanli; Sun, Shumin; Ma, Weibin; Li, Qian; Yang, Yuhui; Wu, Xingquan; Jia, Hang

doi:10.1021/acs.jafc.3c05725

Cited by 10 publications

(12 citation statements)

References 50 publications

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“…In the past, zearalenone hydrolase (ZHD) has been reported to mediate the construction of recombinant carboxypeptidase fusions in ASAG of Bacillus amyloliquefaciens, an enzyme that completely degrades ZEN in pH 7 at 35 • C [44]. In addition, Cheng et al cloned the gene ATM from the HNGD-A6 strain and successfully expressed it in BL21, with a degradation rate of 67.82% for ZEN at a pH of 8.5 and 80 • C [45]. This finding confirms that the L9 strain remains highly active in hot, acidic, and alkaline environments, which is a significant advantage among the currently excavated strains.…”

Section: Discussionmentioning

confidence: 99%

A Novel Bacillus Velezensis for Efficient Degradation of Zearalenone

Li,

Chen,

et al. 2024

Foods

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Zearalenone (ZEN) is considered one of the most serious mycotoxins contaminating grains and their by-products, causing significant economic losses in the feed and food industries. Biodegradation pathways are currently considered the most efficient solution to remove ZEN contamination from foods. However, low degradation rates and vulnerability to environmental impacts limit the application of biodegradation pathways. Therefore, the main research objective of this article was to screen strains that can efficiently degrade ZEN and survive under harsh conditions. This study successfully isolated a new strain L9 which can efficiently degrade ZEN from 108 food ingredients. The results of sequence alignment showed that L9 is Bacillus velezensis. Meanwhile, we found that the L9 degradation rate reached 91.14% at 24 h and confirmed that the primary degradation mechanism of this strain is biodegradation. The strain exhibits resistance to high temperature, acid, and 0.3% bile salts. The results of whole-genome sequencing analysis showed that, it is possible that the strain encodes the key enzyme, such as chitinase, carboxylesterases, and lactone hydrolase, that work together to degrade ZEN. In addition, 227 unique genes in this strain are primarily involved in its replication, recombination, repair, and protective mechanisms. In summary, we successfully excavated a ZEN-degrading, genetically distinct strain of Bacillus velezensis that provides a solid foundation for the detoxification of feed and food contamination in the natural environment.

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Section: Discussionmentioning

confidence: 99%

A Novel Bacillus Velezensis for Efficient Degradation of Zearalenone

Li,

Chen,

et al. 2024

Foods

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“…In order to assess the impact of various cellular components of the isolated strain HB2-2 on the breakdown of AFB 1 , culture supernatants, cell suspensions, and cell extracts were generated following the methodology outlined in a previous work [ 39 ]. The isolated strain HB2-2 was cultured at a temperature of 28 °C for 48 h in NB medium.…”

Section: Methodsmentioning

confidence: 99%

Biological Detoxification of Aflatoxin B1 by Enterococcus faecium HB2-2

Feng,

Cao,

et al. 2024

Foods

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Aflatoxin B1 (AFB1) contamination in food and feed is a global health and economic threat, necessitating the immediate development of effective strategies to mitigate its negative effects. This study focuses on the isolation and characterization of Enterococcus faecium HB2-2 (E. faecium HB2-2) as a potent AFB1-degrading microorganism, using morphological observation, biochemical profiling, and 16S rRNA sequence analysis. An incubation of E. faecium HB2-2 at 32 °C for 96 h in a pH 10 nutrient broth (NB) medium resulted in a remarkable degradation rate of 90.0% for AFB1. Furthermore, E. faecium HB2-2 demonstrated 82.9% AFB1 degradation rate in the peanut meal, reducing AFB1 levels from 105.1 to 17.9 μg/kg. The AFB1 degradation ability of E. faecium HB2-2 was found to be dependent on the fermentation supernatant. The products of AFB1 degradation by E. faecium HB2-2 were analyzed by liquid chromatography–mass spectrometry (LC-MS), and a possible degradation mechanism was proposed based on the identified degradation products. Additionally, cytotoxicity assays revealed a significant reduction in the toxicity of the degradation products compared to the parent AFB1. These findings highlight the potential of E. faecium HB2-2 as a safe and effective method for mitigating AFB1 contamination in food and feed.

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“…AFB 1 metabolites induce cellular oxidative stress, inflammatory responses, mitochondrial damage, and apoptosis in vivo and are mainly excreted from the body through GSTs. Detoxification of AFB 1 in vitro relies mainly on physical, chemical, and microbial treatments such as high temperature, alkaline treatment, and adsorption by yeast Saccharomyces cerevisiae . ,,, These methods have some disadvantages, including difficulty in processing, introduction of new harmful impurities, recontamination of feed with mold, and possible alteration of the original flavor of the food.…”

Section: Summary and Future Perspectivesmentioning

confidence: 99%

Detoxification of Aflatoxin B₁ by Phytochemicals in Agriculture and Food Science

Yang,

Zhang,

Cao

et al. 2024

J. Agric. Food Chem.

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Aflatoxin B 1 (AFB 1 ), the most toxic and harmful mycotoxin, has a high likelihood of occurring in animal feed and human food, which seriously affects agriculture and food safety and endangers animal and human health. Recently, natural plant products have attracted widespread attention due to their low toxicity, high biocompatibility, and simple composition, indicating significant potential for resisting AFB 1 . The mechanisms by which these phytochemicals resist toxins mainly involve antioxidative, anti-inflammatory, and antiapoptotic pathways. Moreover, these substances also inhibit the genotoxicity of AFB 1 by directly influencing its metabolism in vivo, which contributes to its elimination. Here, we review various phytochemicals that resist AFB 1 and their anti-AFB 1 mechanisms in different animals, as well as the common characteristics of phytochemicals with anti-AFB 1 function. Additionally, the shortcomings of current research and future research directions will be discussed. Overall, this comprehensive summary contributes to the better application of phytochemicals in agriculture and food safety.

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Mining Lactonase Gene from Aflatoxin B₁-Degrading Strain Bacillus megaterium and Degrading Properties of the Recombinant Enzyme

Cited by 10 publications

References 50 publications

A Novel Bacillus Velezensis for Efficient Degradation of Zearalenone

A Novel Bacillus Velezensis for Efficient Degradation of Zearalenone

Biological Detoxification of Aflatoxin B1 by Enterococcus faecium HB2-2

Detoxification of Aflatoxin B₁ by Phytochemicals in Agriculture and Food Science

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Mining Lactonase Gene from Aflatoxin B1-Degrading Strain Bacillus megaterium and Degrading Properties of the Recombinant Enzyme

Cited by 10 publications

References 50 publications

A Novel Bacillus Velezensis for Efficient Degradation of Zearalenone

A Novel Bacillus Velezensis for Efficient Degradation of Zearalenone

Biological Detoxification of Aflatoxin B1 by Enterococcus faecium HB2-2

Detoxification of Aflatoxin B1 by Phytochemicals in Agriculture and Food Science

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Product

Resources

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Mining Lactonase Gene from Aflatoxin B₁-Degrading Strain Bacillus megaterium and Degrading Properties of the Recombinant Enzyme

Detoxification of Aflatoxin B₁ by Phytochemicals in Agriculture and Food Science