Mining bifidobacteria from the neonatal gastrointestinal tract for conjugated linolenic acid production

Yang, Bo; Chen, Haiqin; Stanton, Catherine; Chen, Yong Q.; Zhang, Hao; Chen, Wei

doi:10.1080/21655979.2016.1222996

Cited by 20 publications

(15 citation statements)

References 19 publications

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“…While the role of these compounds in CLA production is clear for lactobacilli, research works involving bifidobacterial strains have confirmed their LA/LNAtransforming capacity (47,104,105), but the currently available data suggest that no other metabolites are involved (Fig. 4).…”

Section: Hydratases Are Not Substrate Specificmentioning

confidence: 99%

Microbial Production of Conjugated Linoleic Acid and Conjugated Linolenic Acid Relies on a Multienzymatic System

Salsinha

Pimentel

Fontes

et al. 2018

Microbiol Mol Biol Rev

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Conjugated linoleic acids (CLAs) and conjugated linolenic acids (CLNAs) have gained significant attention due to their anticarcinogenic and lipid/energy metabolism-modulatory effects. However, their concentration in foodstuffs is insufficient for any therapeutic application to be implemented. From a biotechnological standpoint, microbial production of these conjugated fatty acids (CFAs) has been explored as an alternative, and strains of the genera ,, and have shown promising producing capacities. Current screening research works are generally based on direct analytical determination of production capacity (e.g., trial and error), representing an important bottleneck in these studies. This review aims to summarize the available information regarding identified genes and proteins involved in CLA/CLNA production by these groups of bacteria and, consequently, the possible enzymatic reactions behind such metabolic processes. Linoleate isomerase (LAI) was the first enzyme to be described to be involved in the microbiological transformation of linoleic acids (LAs) and linolenic acids (LNAs) into CFA isomers. Thus, the availability of gene sequences has allowed the development of genetic screening tools. Nevertheless, several studies have reported that LAIs have significant homology with myosin-cross-reactive antigen (MCRA) proteins, which are involved in the synthesis of hydroxy fatty acids, as shown by hydratase activity. Furthermore, it has been suggested that CLA and/or CLNA production results from a stress response performed by the activation of more than one gene in a multiple-step reaction. Studies on CFA biochemical pathways are essential to understand and characterize the metabolic mechanism behind this process, unraveling all the gene products that may be involved. As some of these bacteria have shown modulation of lipid metabolism , further research to be focused on this topic may help us to understand the role of the gut microbiota in human health.

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Section: Hydratases Are Not Substrate Specificmentioning

confidence: 99%

Microbial Production of Conjugated Linoleic Acid and Conjugated Linolenic Acid Relies on a Multienzymatic System

Salsinha

Pimentel

Fontes

et al. 2018

Microbiol Mol Biol Rev

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“…Compared with other Bifidobacterium species, B. pseudocatenulatum has been shown to be significantly associated with metabolic diseases in both animal experiments (Agusti et al, 2018) and clinical trials (Wu et al, 2017;Zhao et al, 2018). In addition, some B. pseudocatenulatum strains have been noted for their beneficial properties, such as the production of enterolignan, urolithin and conjugated linoleic acid (Yang et al, 2017;Gaya et al, 2018;Peiroten et al, 2019). Therefore, B. pseudocatenulatum is considered as the next-generation probiotic species for its potential beneficial effects.…”

Section: Introductionmentioning

confidence: 99%

The Diversity of the CRISPR-Cas System and Prophages Present in the Genome Reveals the Co-evolution of Bifidobacterium pseudocatenulatum and Phages

et al. 2020

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Diverse CRISPR-Cas systems constitute an indispensable part of the bacterial adaptive immune system against viral infections. However, to escape from this immune system, bacteriophages have also evolved corresponding anti-defense measures. We investigated the diversity of CRISPR-Cas systems and the presence of prophages in the genomes of 66 Bifidobacterium pseudocatenulatum strains. Our findings revealed a high occurrence of complete CRISPR-Cas systems (62%, 41/66) in the B. pseudocatenulatum genomes. Subtypes I-C, I-U and II-A, were found to be widespread in this species. No significant association was found between the number of bacterial CRISPR spacers and its host's age. This study on prophages within B. pseudocatenulatum genomes revealed that prophage genes related to distinct functional modules became degraded at different levels, indicating that these prophages were not likely to enter lytic cycle spontaneously. Further, the evolutionary analysis of prophages in this study revealed that they might be derived from different phage ancestors. Notably, self-targeting phenomenon within B. pseudocatenulatum and Anti-CRISPR (Acr) coding genes in prophages was observed. Overall, our results indicate that the competition between B. pseudocatenulatum and phages is a major driving factor for the genomic diversity of both partners.

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“…Conjugated linolenic acid has attracted research interests due to its health‐promoting effects. Many bacterial strains have been identified to biosynthesize CLNA from LNA, such as Butyrivibrio fibrisolvens (Kepler and Tove ), Propionibacterium (Hennessy et al ), Bifidobacterium (Coakley et al ; Gorissen et al ; Hennessy et al ; Yang et al ) and Lactobacillus (Kishino et al ). Although a number of lactobacilli have been reported as high CLNA producers, such as L. plantarum (Kishino et al ; Terán et al ), the exact genetic determinant(s) for CLNA production in lactic acid bacteria was unclear.…”

Section: Discussionmentioning

confidence: 99%

Genetic determinates for conjugated linolenic acid production in Lactobacillus plantarum ZS2058

Yang

Chen

et al. 2019

J Appl Microbiol

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Aims: To investigate the genetic determinates for conjugated linolenic acid (CLNA) production in Lactobacillus plantarum ZS2058, a high CLNA producer. Methods and Results: After culturing with a-linolenic acid (ALA) in the medium, the fatty acid compositions of supernatant fluid and cell pellets were analysed via GC-MS. cis9,trans11,cis15-CLNA was identified to be the predominant isomer. And during CLNA production, 10-hydroxy-cis12-cis15octadecenoic acid (10-HOEA) and 10-oxo-cis12-cis15-octadecenoic acid (10-OXOA) were accumulated. The E. coli recombinants harbouring genes encoding myosin-cross-reactive antigen (MCRA), short-chain dehydrogenase/ oxidoreductase (DH) and acetoacetate decarboxylase (DC), respectively, were analysed for their roles in CLNA production. The results indicated that MCRA converted ALA to 10-HOEA, following converted to 10-OXOA by DH. While with the combination of three recombinants, ALA could be transformed into CLNA plus 10-HOEA and 10-OXOA. When the three genes were deleted, none of the L. plantarum ZS2058 knockout mutants could produce any CLNA, after complementation, and all the complementary mutants recovered the CLNAproduction ability at similar levels as the wild strain. Conclusions: Lactobacillus plantarum ZS2058 produced CLNA from ALA with 10-HOEA and 10-OXOA as intermediates. The triple-component isomerase of MCRA, DH and DC was the unique genetic determinant for CLNA generation. Significance and Impact of the Study: The current results firstly provided conclusive evidence that the triple-component isomerase complex was shared by both CLA and CLNA production in lactobacilli. ley et al. 2009;Hennessy et al. 2011;Gorissen et al. 2015;Yang et al. 2015). Generally, CLNA could be divided into two parts according to its sources, which are plant-derived CLNA and microbial-derived CLNA. The most studied plant-derived CLNA included jacaranda acid (cis8,trans10, cis12-CLNA), marigold acid (trans8,trans10,cis12-CLNA), pomegranate acid (cis9,trans11,cis13-CLNA) and eleostearic acid (cis9,trans11,trans13-CLNA) (Yuan et al. 2014). Compared with plant-derived CLNA isomers, microbial CLNA showed different conjugated double bonds, such as cis9,trans11,cis15-CLNA, trans9,trans11,cis15-CLNA and cis6,cis9,trans11-CLNA (Hennessy et al. 2012).

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Mining bifidobacteria from the neonatal gastrointestinal tract for conjugated linolenic acid production

Cited by 20 publications

References 19 publications

Microbial Production of Conjugated Linoleic Acid and Conjugated Linolenic Acid Relies on a Multienzymatic System

Microbial Production of Conjugated Linoleic Acid and Conjugated Linolenic Acid Relies on a Multienzymatic System

The Diversity of the CRISPR-Cas System and Prophages Present in the Genome Reveals the Co-evolution of Bifidobacterium pseudocatenulatum and Phages

Genetic determinates for conjugated linolenic acid production in Lactobacillus plantarum ZS2058

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