Minimum inhibitory concentrations of cephalosporin compounds and their active metabolites for selected mastitis pathogens

Cortinhas, Cristina Simões; Oliveira, L.; Hulland, C.; Santos, Marcos Veiga dos; Ruegg, P.L.

doi:10.2460/ajvr.74.5.683

Cited by 22 publications

(36 citation statements)

References 23 publications

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“…These results are in agreement with data from the literature and confirm the good in vitro activity of cephapirin against Staph. aureus (Salmon et al 1997;Guérin-Faublée et al 2003;Cortinhas et al 2013). Based on its good in vitro activity, the intramammary administration of cephapirin should be a useful tool in controlling clinical and subclinical mastitis.…”

Section: Discussionmentioning

confidence: 98%

Pharmacokinetics in foremilk and antimicrobial activity of cephapirin following intramammary administration in healthy andStaphylococcus aureus-infected cows

Cagnardi

Locatelli

Ferraresi

et al. 2014

New Zealand Veterinary Journal

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The intramammary administration of sodium cephapirin at 275 mg/quarter, twice every 12 hours in lactating cows resulted in higher drug concentrations in milk of quarters with no infection than in the subclinically infected ones. These concentrations were above the MIC90 for 35 hours in infected cows. According to these results intramammary administration of cephapirin at 12-hour intervals during lactation should be potentially effective against Staph. aureus infection, but studies of clinical efficacy are necessary for confirmation.

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Section: Discussionmentioning

confidence: 98%

Pharmacokinetics in foremilk and antimicrobial activity of cephapirin following intramammary administration in healthy andStaphylococcus aureus-infected cows

Cagnardi

Locatelli

Ferraresi

et al. 2014

New Zealand Veterinary Journal

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“…Results for peracetic acid, sodium hypochlorite, and iodine solutions were summarized by calculating the MIC 50 and MIC 90 as described by Cortinhas et al (2013), where MIC 50 and MIC 90 indicate MIC required to inhibit growth of 50% and 90% of the isolates, respectively. Survival analysis was used to determine whether the sanitizers had different MIC.…”

Section: Profile Of Resistance To Sanitizers and Micmentioning

confidence: 99%

Biofilm-producing ability and efficiency of sanitizing agents against Prototheca zopfii isolates from bovine subclinical mastitis

Gonçalves

Lee

Arruda

et al. 2015

Journal of Dairy Science

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The objectives of the present study were to evaluate (1) the capacity of the microalga Prototheca zopfii isolated from subclinical bovine mastitis cases to form biofilms; and (2) the resistance of these isolates to sanitizing agents. Ten isolates of P. zopfii from cows with subclinical mastitis (somatic cell count>200×10(3) cells/mL), distributed in 5 dairy farms, were evaluated for their capacity to form biofilms in polystyrene microplate assays and stainless steel coupons, at 25°C and 37°C±1°C. Prototheca zopfii were isolated from milk samples via microbiological culture and analyzed by 18S rRNA gene sequencing. Biofilm formation on the coupons was observed by scanning electron microscopy. The resistance to sanitizing agents was assessed using the biofilm-forming P. zopfii isolates in stainless steel coupon assays, which were subjected to 3 sanitizers: peracetic acid, sodium hypochlorite, and iodine solution. To evaluate resistance to the sanitizers, the minimum inhibitory concentration (MIC) technique was performed using decreasing concentrations of the sanitizing agents (20, 10, 5, 2.5, 1.25, 0.625, 0.312, 0.156, 0.078, 0.039, and 0.019 g/L). After inoculating the isolates, all concentrations were evaluated at 3 distinct incubation periods (24, 48, and 72 h) to assess the effect of incubation time on the MIC. Using the polystyrene microplate assays, 1 isolate showed weak biofilm production, 5 moderate, and 4 strong, when incubated at 25°C±1. For isolates incubated at 37°C±1, 6 showed weak biofilm production and 4 moderate. All P. zopfii isolates (n=10) had the capacity to form biofilms on stainless steel coupons. The longer the incubation period of the P. zopfii isolates at different dilutions, the greater the concentrations of sanitizer needed to prevent growth of the microalgae under the tested conditions. We detected a significant effect of sanitizer and time of incubation (24, 48, and 72 h) on MIC values against P. zopfii isolates. The isolates were sensitive in vitro to peracetic acid (MIC90≥0.019 g/L), sodium hypochlorite (MIC90≥0.312 g/L), and iodine solution (MIC90≥0.625 g/L), after 24 h of incubation (where MIC90=concentration needed to inhibit 90% of isolates). Of the tested sanitizers, peracetic acid had the greatest efficiency against P. zopfii. We conclude that P. zopfii isolates are capable of biofilm production, which may contribute to their persistence in a milking and dairy environment.

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“…Os testes de suscetibilidade in vitro são utilizados para determinar as concentrações de um agente antimicrobiano que impede o crescimento das bactérias de interesse (CORTINHAS et al, 2013). A avaliação correta das suscetibilidades bacterianas a antibióticos é necessária para uma terapêutica empírica ou dirigida adequada (FERREIRA, 2014).…”

Section: Introductionunclassified

“…A avaliação correta das suscetibilidades bacterianas a antibióticos é necessária para uma terapêutica empírica ou dirigida adequada (FERREIRA, 2014). Cortinhas et al (2013) afirmam que a determinação da concentração inibitória mínima (CIM) é uma importante ferramenta para estudar e comparar a suscetibilidade antimicrobiana de microorganismos contra novas drogas e para monitorar mudanças na resistência ao longo do tempo. Ferreira (2014) afirma que a realização de testes de suscetibilidade aos antimicrobianos é tarefa diária nos laboratórios clínicos mundiais de microbiologia.…”

Section: Introductionunclassified

AVALIAÇÃO DA TENDÊNCIA TEMPORAL DA RESISTÊNCIA BACTERIANA EM ISOLADOS DE Staphylococcus SPP.

Gardelli¹,

Mazucheli²,

Tognim³

et al. 2018

Enci Bio

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Minimum inhibitory concentrations of cephalosporin compounds and their active metabolites for selected mastitis pathogens

Abstract: Differences in inhibition between parent compounds and their active metabolites may be responsible for some of the variation between clinical outcomes and results of in vitro susceptibility tests.

Cited by 22 publications

References 23 publications

Pharmacokinetics in foremilk and antimicrobial activity of cephapirin following intramammary administration in healthy andStaphylococcus aureus-infected cows

Pharmacokinetics in foremilk and antimicrobial activity of cephapirin following intramammary administration in healthy andStaphylococcus aureus-infected cows

Biofilm-producing ability and efficiency of sanitizing agents against Prototheca zopfii isolates from bovine subclinical mastitis

AVALIAÇÃO DA TENDÊNCIA TEMPORAL DA RESISTÊNCIA BACTERIANA EM ISOLADOS DE Staphylococcus SPP.

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