Minimum endotoxin concentration causing inflammation in the anterior segment of rabbit eyes

Abstract

There was a dissociation in response between flare and cells in the aqueous to intracameral endotoxin. The minimum endotoxin concentration causing inflammation ranged between 0.23 and 0.60 EU.

Determination of a No-Observable Effect Level for Endotoxin Following a Single Intravitreal Administration to Dutch Belted Rabbits

“…The rapid time course of the reaction is in line with data published by several other groups following both intracameral and intravitreal endotoxin injections. 3 In vehicle-treated eyes, a single eye was noted with grade 2þ aqueous flare at 24 hours post dose, consistent with the peak onset of aqueous flare in eyes treated with endotoxin. A high amount of flare using LFP (359.1 ph/ms) confirmed the clinical finding of aqueous flare at this interval.…”

“…Sakimoto et al 3 used slit-lamp ophthalmoscopy and LFP to identify a NOEL of 0.23 EU/eye following intracameral administration of endotoxin. However, other groups have reported inflammatory response following intracameral injection of OVDs containing ‡0.02 EU/eye levels of endotoxin.…”

“…The purpose of this 43-day study was to define the response to intravitreal purified reference endotoxin levels of 0.01 to 0.75 endotoxin unit/eye (EU/eye) in Dutch Belted rabbits, including the definition of a no-observable effect level (NOEL) and the time course of the response in both the anterior and posterior segments of the eye. As mentioned above, adverse ocular effects following intracameral endotoxin and endotoxin-contaminated OVDs in rabbits have been described previously, [3][4][5] and generally was used as the basis for dose range selection in the current study.…”

“…The eye has been shown to be very sensitive to the effects of intracameral endotoxin and endotoxin-contaminated ophthalmic viscosurgical devices (OVDs). [3][4][5] The inflammatory effects of endotoxin are well described, and it is often used to produce experimental animal models of uveitis following ITV in mice, 6 rabbits, [7][8][9][10][11] pigs, 12 and monkeys. 13 Clinically, cases of acute postoperative sterile uveitis or toxic anterior segment syndrome have been linked to endotoxin contamination of ophthalmic devices.…”

Avaliação in vitro da citotoxicidade de instrumentos cirúrgicos enxaguados com diferentes qualidades de água.

“…Embora a água de torneira utilizada nesta pesquisa apresentasse 4,70 UE/mL, inferior ao limite de 10 UE/mL da AAMI (2007), é preciso ressaltar que o endotélio ocular é extremamente sensível às endotoxinas. Sakimoto et al (2009) (Alves et al, 2013).…”