MiniMax, a new diminutive Glycine max genotype with a rapid life cycle, embryogenic potential and transformation capabilities

Abstract

We developed Glycine max cv MiniMax (PI643148) that has a rapid life cycle, short stature and characteristic simple sequence repeat (SSR) markers that could make it useful for mutant screening, functional genomics, genetic mapping and other studies involving soybeans. We demonstrate that MiniMax is able to make somatic embryos (SEs) that rapidly develop into plantlets. Thus, the rapid cycling habit carries over into aspects of plant regeneration. Chimaeras (having transformed roots with untransformed aerial stocks) have been produced rapidly under non-axenic conditions using Agrobacterium rhizogenes-mediated transformation. Part of these experiments involved the engineering an enhanced green fluorescent protein (eGFP) reporter cassette outside the multi-cloning site of a plant expression vector, permitting non-invasive visual screening of the transformed roots. The rapid cycling growth habit of MiniMax, its ability to efficiently generate SEs and ability to be transformed should prove useful for basic aspects of G. max molecular and genetic research.

A plant transformation system designed for high throughput genomics inGossypium hirsutumto study root–organism interactions

“…By using FMV-sgt as the promoter, the cassette is designed to drive the overexpression of full length genes and maintain high expression throughout plant-organism interactions (Klink et al 2008(Klink et al , 2009Matsye et al 2012;Pant, Matsye, et al 2014;). The pRAP15 vector was engineered to have the tetracycline resistance gene for bacterial selection, found to be important for work with A. rhizogenes (Klink et al 2008). The tetracycline resistance gene was engineered into a BstEII site that lies outside the left and right borders.…”

“…rhizogenes-mediated nonaxenic transformation A modified version of the nonaxenic hairy root plant transformation procedure was developed here for use in G. hirsutum (Tepfer 1984;Klink et al 2008Klink et al , 2009Matsye et al 2012;Pant, Matsye, et al 2014;. Seeds of G. hirsutum were planted in prewetted sterilized sand, germinated, and grown for 14 days at ambient greenhouse temperatures (~26-29°C).…”

“…In addition to its capability of being used under nonaxenic conditions, the platform presented here has the added feature of being compatible with the Gateway® technology (Klink et al 2008;Matsye et al 2012). The compatibility with the Gateway® technology allows for large numbers of genes to be directionally cloned first into the entry vector and then shuttled into pRAP15 overexpression vector.…”

Soybean: Plant Manipulation to Agrobacterium Mediated Transformation

“…This can further be used for high throughput transformation, to test high number of genes, different biological processes and symbiotic relation etc. Klink et al (2008) introduced a new soybean variety MiniMax with a rapid and short life cycle that produced hairy roots under non-axenic conditions when infected with A. rhizogens strains K599 harboring disarmed vector pKSF3. These transgenic roots were capable of compatible reactions with several Heterodera glycines races.…”

Cell-Specific Studies of Soybean Resistance to Its Major Pathogen, the Soybean Cyst Nematode as Revealed by Laser Capture Microdissection, Gene Pathway Analyses and Functional Studies

“…Some of the studies involve a procedure for rapid and efficient transformation (Sun et al 2006: Orzaez et al 2006. The development of the soybean genotype MiniMax (PI 643148), a dwarf, rapid cycling, MG 000 plant that grows as quickly and is as tall as the genetic model A. thaliana (Matthews et al 2007;Klink et al 2008) may fill that void (Figure 7). MiniMax is well suited for forward genetic analyses that include the development of NILs and RILs because they can be grown year-round in the greenhouse with little effort (Klink et al 2008).…”