Minimally Invasive Nasal Sampling in Children Offers Accurate Pneumococcal Colonization Detection

Nikolaou, Elissavet; Blizard, Annie; Pojar, Sherin; Mitsi, Elena; German, Esther L.; Reiné, Jesús; Hill, Helen; McNamara, Paul; Collins, Andrea; Ferreira, Daniela M.; Jochems, Simon P.

doi:10.1097/inf.0000000000002454

Cited by 7 publications

(9 citation statements)

References 9 publications

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“…DNA extraction was performed using magnetic beads, as previously published. 17 To determine the colonization of S. pneumoniae and H. influenzae , qPCR was performed using the LytA and IgA1 gene, respectively, as previously described. 17,18 qPCR for the copB gene was performed to determine colonization of M. catarrhalis , and for S. aureus , the nuc gene was used as previously described.…”

Section: Methodsmentioning

confidence: 99%

Differences in Bacterial Colonization and Mucosal Responses Between High and Low SES Children in Indonesia

Dorst

Azimi

Wahyuni

et al. 2022

Pediatric Infectious Disease Journal

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Background: Increased nasopharyngeal carriage of pathogenic bacteria is found in low socioeconomic status (SES) settings. How SES affects local immune responses, important for controlling colonization, is currently unknown. Objective: Examining bacterial colonization and cytokine response in the nasal mucosa of children from high and low SES. Methods: Nasosorption samples were collected in October 2019 from 48 high SES and 50 low SES schoolchildren, in a cross-sectional study in Makassar, Indonesia. Twenty-five cytokines were measured in nasal fluid. Quantitative polymerase chain reaction was performed to determine carriage and density of Haemophilus influenzae, Streptococcus pneumoniae, Moraxella catarrhalis and Staphylococcus aureus. Data were analyzed using multivariate regression. Results: H. influenzae and S. pneumoniae densities were increased in low SES settings compared to the high SES settings (P = 0.006, P = 0.026), with 6 and 67 times higher median densities, respectively. Densities of H. influenzae and S. pneumoniae were positively associated with levels of IL-1beta and IL-6. After correcting for bacterial density, IL-6 levels were higher in colonized children from high SES than low SES for H. influenzae and S. pneumoniae (both P = 0.039). Conclusion:Increased densities of H. influenzae and S. pneumoniae were observed in low SES children, whereas IL-6 levels associated with colonization were reduced in these children, indicating that immune responses to bacterial colonization were altered by SES.

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Section: Methodsmentioning

confidence: 99%

Differences in Bacterial Colonization and Mucosal Responses Between High and Low SES Children in Indonesia

Dorst

Azimi

Wahyuni

et al. 2022

Pediatric Infectious Disease Journal

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“…Isolation of bacteria from colonized children. Nasopharyngeal swab samples from a previously described pediatric cohort (36) were analyzed for bacterial RNA expression. This study was approved by the National Health Service Research and Ethics Committee (17/NW/0663).…”

Section: Methodsmentioning

confidence: 99%

Targeted Transcriptomic Screen of Pneumococcal Genes Expressed during Murine and Human Infection

et al. 2022

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“…To determine nasal bacterial colonization, qPCR was performed on the bacterial pellet. DNA extraction was performed using magnetic beads, as previously published (17). To determine the colonization of S. pneumoniae and H. influenzae, qPCR was performed using the LytA and IgA1 gene respectively, as previously described (17, 18).…”

Section: Methodsmentioning

confidence: 99%

“…DNA extraction was performed using magnetic beads, as previously published (17). To determine the colonization of S. pneumoniae and H. influenzae, qPCR was performed using the LytA and IgA1 gene respectively, as previously described (17, 18). qPCR for the copB gene was performed to determine colonization of M. catarrhalis and for S. aureus the nuc gene was used as previously described (6).…”

Section: Methodsmentioning

confidence: 99%

Differences in bacterial colonization and mucosal responses between high and low SES children in Indonesia

Dorst

Azimi

Wahyuni

et al. 2021

Preprint

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BACKGROUNDNasopharyngeal carriage of pathogenic bacteria precedes invasive disease and higher rates are found in low socioeconomic-status (SES) settings. Local immune responses are important for controlling colonization, but whether SES affects these responses is currently unknown.OBJECTIVEExamining bacterial colonization and cytokine response in nasal mucosa of children from high and low SES.METHODSTwenty-five cytokines were measured in nasal fluid. qPCR was performed to determine carriage and density of Haemophilus influenzae (H. influenzae), Streptococcus pneumoniae (S. pneumoniae), Moraxella catarrhalis (M. catarrhalis) and Staphylococcus aureus (S. aureus).RESULTSThe densities of H. influenzae and S. pneumoniae were increased in low compared to the high SES (p=0.006, p=0.026), with respectively 6 and 67 times higher median densities. Densities of H. influenzae and S. pneumoniae were positively associated with levels of IL-1beta (p=0.002, p=0.008) and IL-6 (p<0.001, p=0.006). After correcting for bacterial density, IL-6 levels were increased in colonized children from high compared to low SES for both H. influenzae and S. pneumoniae (both p=0.039).CONCLUSIONIncreased density of H. influenzae and S. pneumoniae was observed in low SES children, while IL-6 levels associated with colonization were reduced in these children, indicating that immune responses to bacterial colonization were altered by SES.

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Minimally Invasive Nasal Sampling in Children Offers Accurate Pneumococcal Colonization Detection

Cited by 7 publications

References 9 publications

Differences in Bacterial Colonization and Mucosal Responses Between High and Low SES Children in Indonesia

Differences in Bacterial Colonization and Mucosal Responses Between High and Low SES Children in Indonesia

Targeted Transcriptomic Screen of Pneumococcal Genes Expressed during Murine and Human Infection

Differences in bacterial colonization and mucosal responses between high and low SES children in Indonesia

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