Minimally Invasive LVAD Surgery in Septuagenarians

Rojas, S. V.; Avsar, M.; Hanke, Jasmin S.; Deniz, E.; Meier, A; Tümler, K.; Martens, A.; Cebotari, S; Haverich, Axel; Schmitto, J

doi:10.1055/s-0035-1544298

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“…Adult CMs are minimally proliferative, with only about 1% of the cells proliferating per year in an adult heart . The current methods of treatment for heart failure are limited; left ventricular assist devices only provide temporary aid, and cardiac transplants are constrained by a shortage of donor hearts . The proliferation and differentiation potential of human pluripotent stem cell (hPSC)‐derived cardiac cells and tissues provides a novel strategy to restore contractility to a failing heart …”

Section: Introductionmentioning

confidence: 99%

Coculture of Endothelial Cells with Human Pluripotent Stem Cell‐Derived Cardiac Progenitors Reveals a Differentiation Stage‐Specific Enhancement of Cardiomyocyte Maturation

Dunn

Reichardt

Simmons

et al. 2019

Biotechnology Journal

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Cardiomyocytes (CMs) generated from human pluripotent stem cells (hPSCs) are immature in their structure and function, limiting their potential in disease modeling, drug screening, and cardiac cellular therapies. Prior studies have demonstrated that coculture of hPSC‐derived CMs with other cardiac cell types, including endothelial cells (ECs), can accelerate CM maturation. To address whether the CM differentiation stage at which ECs are introduced affects CM maturation, the authors coculture hPSC‐derived ECs with hPSC‐derived cardiac progenitor cells (CPCs) and CMs and analyze the molecular and functional attributes of maturation. ECs have a more significant effect on acceleration of maturation when cocultured with CPCs than with CMs. EC coculture with CPCs increases CM size, expression of sarcomere, and ion channel genes and proteins, the presence of intracellular membranous extensions, and chronotropic response compared to monoculture. Maturation is accelerated with an increasing EC:CPC ratio. This study demonstrates that EC incorporation at the CPC stage of CM differentiation expedites CM maturation, leading to cells that may be better suited for in vitro and in vivo applications of hPSC‐derived CMs.

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Section: Introductionmentioning

confidence: 99%