Minimally Invasive Intradiscal Delivery of BM-MSCs via Fibrous Microscaffold Carriers

Nakielski, Paweł; Rybak, Daniel; Jezierska-Woźniak, Katarzyna; Rinoldi, Chiara; Sinderewicz, Emilia; Staszkiewicz-Chodor, Joanna; Haghighat Bayan, Mohammad Ali; Czelejewska, Wioleta; Urbanek, Olga; Kosik-Kozioł, Alicja; Barczewska, Monika; Skomorowski, Mateusz; Holak, Piotr; Lipiński, Seweryn; Maksymowicz, Wojciech; Pierini, Filippo

doi:10.1021/acsami.3c11710

Cited by 4 publications

(1 citation statement)

References 60 publications

(107 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, laser structuring and insights drwan from biological structures play crucial roles in the creation of novel designs. Laser structuring enables the production of scaffolds with precise geometric shapes using repeatable techniques. , Additionally, leveraging biological inspiration enhances electrospun membranes for diverse applications, including quick-drying materials . Through further interdisciplinary research, electrospun nanofibers can unleash their full potential as a transformative technology with diverse applications in healthcare and beyond.…”

Section: Discussionmentioning

confidence: 99%

Advances in Electrospun Nanofibers: Versatile Materials and Diverse Biomedical Applications

Wang,

Su,

Xie

2024

Acc. Mater. Res.

View full text Add to dashboard Cite

Metrics & MoreArticle Recommendations * sı Supporting Information CONSPECTUS: Electrospining has emerged as a versatile and transformative technique for the fabrication of nanofiber materials, which have been shown to be promising in applications across various biomedical domains. Cutting-edge research in electrospinning technology is centered on enhancing versatility, efficiency, and functionality of electrospun nanofibers through several key facets. These include the development of advanced materials, with ongoing exploration into novel polymer systems spanning synthetic polymers, natural polymers, and polymer blends to broaden the spectrum of achievable properties and functions. Additionally, there is significant emphasis on controlling fiber size, morphology, and alignment. Surface functionalization with bioactive molecules, drugs, or targeting ligands enhances specific functionalities like antimicrobial properties, cell adhesion, or targeted drug delivery. Furthermore, researchers are delving into the creation of multifunctional hybrid structures by integrating electrospinning with other fabrication techniques such as 3D printing, microfluidics, or layer-by-layer assembly, enabling customized properties and functionalities. Lastly, there is a strong fucus on biomedical applications, leveraging electrospun nanofibers for tissue engineering, wound healing, drug delivery, and biosensing, aiming to develop biocompatible and bioresorbable scaffolds with controlled structural and bioactive cues to promote tissue regeneration and repair. However, several significant questions remain unanswered. For instance, can electrospun nanofibers sustainably deliver immunomodulating compounds topically to enhance human innate immunity? Is it possible to develop a robust approach for fabricating 3D nanofiber scaffolds with precise shapes and controlled characteristics such as fiber alignment, porosity, and pore size? Can these scaffolds be implanted into the body using minimal invasive surgery considering their current requirement for invasive surgical implantation? Additionally, can electrospun nanofibers be processed into microspheres or microcarriers for injectable therapy, and how effectively can they integrate with other technologies? Over the past decade, our laboratory has focused on addressing these questions. In this Account, we present an overview of recent developments of electrospun nanofiber materials, emphasizing their modifications, unique structures, integration with other technologies, and diverse biomedical applications. We begin by outlining and comparing three methods for modifying electrospun nanofibers, laying the groundwork for selecting the most appropriate technique. Subsequently, we summarize methods for engineering novel forms of electrospun nanofiber materials. Further, we explore the integration of electrospun nanofibers with other technologies, such as microfluidic chips, microneedles, and electrostatic flocking. Following this, we highlight several notable biomedical applications, including infect...

show abstract

Section: Discussionmentioning

confidence: 99%

Advances in Electrospun Nanofibers: Versatile Materials and Diverse Biomedical Applications

Wang,

Su,

Xie

2024

Acc. Mater. Res.

View full text Add to dashboard Cite

show abstract

Propelling Minimally Invasive Tissue Regeneration With Next‐Era Injectable Pre‐Formed Scaffolds

Liao,

Timoshenko,

Cordova

et al. 2024

Advanced Materials

View full text Add to dashboard Cite

The growing aging population, with its associated chronic diseases, underscores the urgency for effective tissue regeneration strategies. Biomaterials have played a pivotal role in the realm of tissue reconstruction and regeneration, with a distinct shift towards minimally invasive (MI) treatments. This transition, fueled by engineered biomaterials, has steered away from invasive surgical procedures to embrace approaches offering reduced trauma, accelerated recovery, and cost‐effectiveness. In the realm of MI tissue repair and cargo delivery, various techniques have been explored. While in situ polymerization has been prominent, it is not without its challenges. This narrative review explores diverse biomaterials, fabrication methods, and biofunctionalization for injectable pre‐formed scaffolds, focusing on their unique advantages. The injectable pre‐formed scaffolds, exhibiting compressibility, controlled injection, and maintained mechanical integrity, emerge as promising alternative solutions to in situ polymerization challenges. The conclusion of this review emphasizes the importance of interdisciplinary design facilitated by synergizing fields of materials science, advanced 3D biomanufacturing, and mechanobiological studies, and innovative approaches for effective MI tissue regeneration.This article is protected by copyright. All rights reserved

show abstract

Preparation of Short Collagen Nanofibers for Injectable Hydrogels: Comparative Assessment of Fragmentation Methods, Physicomechanical Properties, and Biocompatibility

Karimizade,

Mellati

2024

Macro Materials & Eng

View full text Add to dashboard Cite

Collagen nanofibers can be employed in hydrogels to create injectable nanocomposite hydrogels, mimicking the fibrous architecture of the natural extracellular matrix (ECM). As long continuous electrospun collagen nanofibers are not applicable, fragmentation is inevitable to obtain injectable hydrogels with a fine viscosity. Here, four methods: hand grinding (HG), homogenizer (HM), mixer milling (MM), and ultrasonication (UH) are used to disintegrate and shorten collagen nanofiber mats before incorporation into an injectable hyaluronic acid hydrogel as a matrix. The Length‐to‐diameter (L/d) ratio and morphology of fragmented collagen are compared by SEM. The injection force, mechanical properties, and cell viability of the selected collagen‐incorporated hydrogels are also evaluated. UH emerges as the most effective method, yielding the highest L/d ratio of 46 and a notable compressive modulus of 8.7 ± 0.92 kPa. Assessment of the in vitro cell viability of the encapsulated chondrocytes in the collagen‐incorporated hydrogels demonstrates good biocompatibility, and hydrogels containing UH short nanofiber, in particular, show an increase in cell proliferation. This work indicates how collagen mats can be effectively broken down and combined with injectable hydrogels to enhance both their mechanical behavior and biocompatibility.

show abstract

Minimally Invasive Intradiscal Delivery of BM-MSCs via Fibrous Microscaffold Carriers

Cited by 4 publications

References 60 publications

Advances in Electrospun Nanofibers: Versatile Materials and Diverse Biomedical Applications

Advances in Electrospun Nanofibers: Versatile Materials and Diverse Biomedical Applications

Propelling Minimally Invasive Tissue Regeneration With Next‐Era Injectable Pre‐Formed Scaffolds

Preparation of Short Collagen Nanofibers for Injectable Hydrogels: Comparative Assessment of Fragmentation Methods, Physicomechanical Properties, and Biocompatibility

Contact Info

Product

Resources

About