Minimally invasive implantation and decreased inflammation reduce osteoinduction of biomaterial

Zhao, Zifan; Zhao, Qin; Gu, Bin; Yin, Chengcheng; Shen, Kailun; Tang, Hua; Xia, Haibin; Zhang, Xiaoxin; Zhao, Yufang; Yang, Xiangliang; Zhang, Yufeng

doi:10.7150/thno.39507

Cited by 32 publications

(29 citation statements)

References 48 publications

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“…Especially in bone tissue, the role of macrophages in interfering with the engraftment process has been shown by researchers to be particularly frequent, i.e., they are a target [28][29][30].…”

Section: Discussionmentioning

confidence: 99%

Changes in Spectral Fluorescence Properties of a Near-Infrared Photosensitizer in a Nanoform as a Coating of an Optical Fiber Neuroport

et al. 2021

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In this work, we tested a new approach to assess the presence of inflammatory process in the implant area using spectral methods and the technique of fiber fluorescence analysis of photosensitizers in nanoform. First of all, the spectral characteristics of the photosensitizer when interacting with the porous surface of the implant, based on hydroxyapatite under in vitro and in vivo conditions, were determined. Thus, it was shown that spectral characteristics of photosensitizers can be used for judgement on the process of inflammation in the implant area and thus on the local presence of the immunocompetent cells. The analysis was performed at a sufficient depth in the biotissue by using the near-infrared spectral region, as well as two different methods: fiber-based laser spectroscopy and fiber-optic neuroscopy, which served to monitor the process and regular fluorescence diagnosis of the studied area. Fluorescence spectroscopic analysis was performed on experimental animals in vivo, i.e., under conditions of active immune system intervention, as well as on cell cultures in vitro in order to judge the role of the immune system in the interaction with the implant in comparison. Thus, the aim of the study was to determine the relationship between the fluorescence signal of nanophotosensitizers in the near infrared spectral region and its parameters with the level of inflammation and the type of surface with which the photosensitizer interacts in the implant area. Thus, fiber-optic control opens up new approaches for further diagnosis and therapy in the implant area, making immune cells a prime target for advanced therapies.

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Section: Discussionmentioning

confidence: 99%

Changes in Spectral Fluorescence Properties of a Near-Infrared Photosensitizer in a Nanoform as a Coating of an Optical Fiber Neuroport

et al. 2021

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“…Osteo-induction medium (OS) consisted of α-MEM supplemented with 10% FBS, 10 mmol/L β-glycerophosphate, 10 nM dexamethasone, and 50 μg/mL ascorbic acid (Sigma, St. Louis, MS, USA) [ 2 ]. BMSCs (2 × 10 5 cells/well) were seeded in 12-well plates.…”

Section: Methodsmentioning

confidence: 99%

“…In the field of bone engineering, ideal bone substitutes, such as bioactive ceramics, are designed to enhance stem cell recruitment, attachment, and osteogenic differentiation [ 1 ]. In addition to the direct effects on stem cell, bone substitutes are assumed to create pro-regenerative niche to support stem cell proliferation and osteogenic differentiation [ 2 ]. Hence, it is important to uncover the interaction between bone substitutes and the surrounding niche after inner implantation.…”

Section: Introductionmentioning

confidence: 99%

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Fibroblasts Mediate Ectopic Bone Formation of Calcium Phosphate Ceramics

Zhao

et al. 2022

Materials

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Heterogeneity of fibroblasts directly affects the outcome of tissue regeneration; however, whether bioactive ceramics regulate bone regeneration through fibroblasts is unclear. Ectopic bone formation model with biphasic calcium phosphate (BCP) implantation was used to investigate the temporal and spatial distribution of fibroblasts around ceramics. The effect of BCP on L929 fibroblasts was evaluated by EdU assay, transwell assay, and qRT-PCR. Further, the effect of its conditioned medium on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) was confirmed by ALP staining. SEM and XRD results showed that BCP contained abundant micro- and macro-pores and consisted of hydrogen-apatite (HA) and β-tricalcium phosphate (β-TCP) phases. Subsequently, BCP implanted into mice muscle successfully induced osteoblasts and bone formation. Fibroblasts labelled by vimentin gathered around BCP at 7 days and peaked at 14 days post implantation. In vitro, BCP inhibited proliferation of L929 fibroblast but promoted its migration. Moreover, expression of Col1a1, Bmp2, and Igf1 in L929 treated by BCP increased significantly while expression of Tgfb1 and Acta did not change. ALP staining further showed conditioned media from L929 fibroblasts treated by BCP could enhance osteogenic differentiation of BMSCs. In conclusion, fibroblasts mediate ectopic bone formation of calcium phosphate ceramics.

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“…CCL5, CCL2, and IL-8 secreted by macrophages can promote the migration of MSCs to bone injury sites, and these soluble cytokines play a role by activating the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) signal pathway [ 22 , 31 ]. T cells stimulated by TNF- α activate the nuclear factor-kappa B (NF- κ B) pathway and secrete more CCL5 to recruit MSCs to the injured site, thereby achieving the effect of ectopic osteogenesis [ 32 ]. Ponte et al [ 25 ] studied the effects of 16 chemokines and growth factors on the migration of MSCs in vitro and agreed that TNF and IL-1 β can cause MSCs to express more chemokine receptors; consequently, their sensitivity to chemokines improves.…”

Section: Osteoinductive Effects Of Immune Cells On Mscs For Bone Rege...mentioning

confidence: 99%

Mesenchymal Stem Cell–Immune Cell Interaction and Related Modulations for Bone Tissue Engineering

Chen

Hao

Wang

et al. 2022

Stem Cells International

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Critical bone defects and related delayed union and nonunion are still worldwide problems to be solved. Bone tissue engineering is mainly aimed at achieving satisfactory bone reconstruction. Mesenchymal stem cells (MSCs) are a kind of pluripotent stem cells that can differentiate into bone cells and can be used as one of the key pillars of bone tissue engineering. In recent decades, immune responses play an important role in bone regeneration. Innate immune responses provide a suitable inflammatory microenvironment for bone regeneration and initiate bone regeneration in the early stage of fracture repair. Adaptive immune responses maintain bone regeneration and bone remodeling. MSCs and immune cells regulate each other. All kinds of immune cells and secreted cytokines can regulate the migration, proliferation, and osteogenic differentiation of MSCs, which have a strong immunomodulatory ability to these immune cells. This review mainly introduces the interaction between MSCs and immune cells on bone regeneration and its potential mechanism, and discusses the practical application in bone tissue engineering by modulating this kind of cell-to-cell crosstalk. Thus, an in-depth understanding of these principles of bone immunology can provide a new way for bone tissue engineering.

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Minimally invasive implantation and decreased inflammation reduce osteoinduction of biomaterial

Cited by 32 publications

References 48 publications

Changes in Spectral Fluorescence Properties of a Near-Infrared Photosensitizer in a Nanoform as a Coating of an Optical Fiber Neuroport

Changes in Spectral Fluorescence Properties of a Near-Infrared Photosensitizer in a Nanoform as a Coating of an Optical Fiber Neuroport

Fibroblasts Mediate Ectopic Bone Formation of Calcium Phosphate Ceramics

Mesenchymal Stem Cell–Immune Cell Interaction and Related Modulations for Bone Tissue Engineering

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