Minimally Invasive and Portable Method for the Identification of Proteins in Ancient Paintings

Cicatiello, Paola; Ntasi, Georgia; Rossi, Manuela; Marino, Gennaro; Giardina, Paola; Birolo, Leila

doi:10.1021/acs.analchem.8b01718

Cited by 38 publications

(15 citation statements)

References 25 publications

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“…Recently, a number of additional minimally destructive methods have been developed for art and cultural heritage materials. These include polishing films with grit ( 25 ), ethylene vinyl acetate films studded with strong cation and anion exchangers and C8/C18 resins ( 102 ), and enzyme functionalized films ( 103 ) and hydrogels ( 27 ).…”

Section: Zooms: Methods and State Of The Fieldmentioning

confidence: 99%

A primer for ZooMS applications in archaeology

Warinner

2022

Proc. Natl. Acad. Sci. U.S.A.

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Collagen peptide mass fingerprinting by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, also known as zooarchaeology by mass spectrometry (ZooMS), is a rapidly growing analytical technique in the fields of archaeology, ecology, and cultural heritage. Minimally destructive and cost effective, ZooMS enables rapid taxonomic identification of large bone assemblages, cultural heritage objects, and other organic materials of animal origin. As its importance grows as both a research and a conservation tool, it is critical to ensure that its expanding body of users understands its fundamental principles, strengths, and limitations. Here, we outline the basic functionality of ZooMS and provide guidance on interpreting collagen spectra from archaeological bones. We further examine the growing potential of applying ZooMS to nonmammalian assemblages, discuss available options for minimally and nondestructive analyses, and explore the potential for peptide mass fingerprinting to be expanded to noncollagenous proteins. We describe the current limitations of the method regarding accessibility, and we propose solutions for the future. Finally, we review the explosive growth of ZooMS over the past decade and highlight the remarkably diverse applications for which the technique is suited.

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Section: Zooms: Methods and State Of The Fieldmentioning

confidence: 99%

A primer for ZooMS applications in archaeology

Warinner

2022

Proc. Natl. Acad. Sci. U.S.A.

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“…After a ZipTip purification step, the MALDI-MS spectrum of the peptide mixture was acquired (Figure 5). The resulting PMF was interpreted as follows; most peak signals were typical of collagen peptides, among which, for example, peaks at m/z 1105.4, 1267.5, 1427.5, 1459.5, and 2130.8 were due to peptides of collagen α-1(I) or collagen α-2(I) chains [20,21,[38][39][40]. A detailed interpretation of MS signals is reported in Table 1.…”

Section: Angel's Viola Brown Layer (Sampled Point 2)mentioning

confidence: 99%

Multi-Technique Characterization of Pictorial Organic Binders on XV Century Polychrome Sculptures by Combining Micro- and Non-Invasive Sampling Approaches

et al. 2021

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A stony sculptural composition of the Nativity Scene is preserved in Altamura’s Cathedral (Apulia, Italy). This commonly called Apulian “presepe”, attributed to an unknown stonemason, is composed of polychrome carbonate white stone sculptures. While earlier stratigraphic tests have unveiled a complex superimposition of painting layers—meaning that several editions of the sculptures succeeded from the 16th to 20th century—a chemical investigation intended to identify the organic binding media used in painting layers was undertaken. Drawing on current literature, two strategies were exploited: a non-invasive in situ digestion analysis and an approach based on micro-removal of painting film followed by the Bligh and Dyer extraction protocol. Both peptide and lipid mixtures were analyzed by matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) and reversed-phase liquid chromatography coupled to mass spectrometry by electrospray ionization (RPLC-ESI-MS). Attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR) examinations were also performed on micro-samples of painting films before lipids and proteins extraction. While human keratins were found to be common contaminants of the artwork’s surfaces, traces of animal collagen, siccative oils, and egg white proteins were evidenced in different sampling zones of the sculptures, thus suggesting the use of non-homogeneous painting techniques in the colored layers.

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“…In this context, MS coupled with soft ionization sources (i.e., MALDI, ESI) may be considered the best analytical tool for macromolecules structural investigation, including proteins and lipids, because it provides molecular weights as well as specific fragmentation patterns in tandem mass experiments. Starting from 2017, research was focused on the exploration of innovative minimally invasive approaches for pictorial binders' identification as novel film in vinyl-acetate [8][9][10] or fungal hydrofobins adhered on cellulose acetate sheets [11,12]. In 2020 we proposed a minimally invasive procedure for proteinaceous binders' identification which is based on a poly(2hydroxyethylmethacrylate)/poly(vinylpyrrolidone) (pHEMA/PVP) hydrogel loaded with trypsin.…”

Section: Introductionmentioning

confidence: 99%

Historical proteinaceous-based pictorial binders’ analysis: a novel proteomic minimally invasive approach

Rigante

Calvano

Sabbatini

et al. 2022

J. Phys.: Conf. Ser.

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Operating micro-invasive sampling when dealing with the chemical investigation of precious historical works of art is, without doubts, mandatory. Recently, scientific research in the field of Cultural Heritage has been often devoted to the development of innovative strategies for quasi non-invasive sampling on works of art to extract, for example, proteins and/or lipids composing the pictorial binders gaining extensive awareness about the materials employed by the artist. In this work, we propose a novel minimally invasive method for the sampling and characterization of protein-based painting binders. We apply a hydrophilic gel, previously functionalized with trypsin and chymotrypsin, on the artwork surface to perform an in-situ digestion of proteinaceous binders. Peptides are identified by means of a proteomic bottom-up approach combined with a high resolution/accuracy mass spectrometry technique. A wooden polychrome statue belonging to the XV century was chosen as a sample to assess the proposed protocol on a historical painting layer, as illustrated herein.

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Minimally Invasive and Portable Method for the Identification of Proteins in Ancient Paintings

Cited by 38 publications

References 25 publications

A primer for ZooMS applications in archaeology

A primer for ZooMS applications in archaeology

Multi-Technique Characterization of Pictorial Organic Binders on XV Century Polychrome Sculptures by Combining Micro- and Non-Invasive Sampling Approaches

Historical proteinaceous-based pictorial binders’ analysis: a novel proteomic minimally invasive approach

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