Minimally Functional Rat Adrenal Medullary Pheochromocytomas

Ribelin, William E.; Roloff, Marston Val; Houser, Robert M.

doi:10.1177/030098588402100303

Cited by 12 publications

(4 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although serum or urine catecholamine or catecholamine metabolite levels were not available for the animals studied, biochemically functional tumors frequently do not produce detectable elevations of circulating catecholamines if their secretory activity is low, as is apparently often the case for pheochromocytomas in rats. 27 In this study, we did not stain for DBH and therefore cannot be certain whether tumors that were TH-positive but PNMT-negative produced norepinephrine or dopamine. Studies of both human19.22 and rat38 adrenal glands, however, have shown that most pheochromocytomas that are positive for TH are also positive for DBH.…”

Section: Discussionmentioning

confidence: 81%

Immunohistochemical and Morphological Characterization of Spontaneously Occurring Pheochromocytomas in the Aging Mouse

1996

View full text Add to dashboard Cite

Abstract. Pheochromocytomas in mice are rare tumors, and their expression of functional markers has not previously been assessed. In this study, 29 spontaneously occurring mouse pheochromocytomas were characterized morphologically and immunohistochemically to determine whether there are functional correlates to previously described morphological features and to provide a database for comparison with tumors that arise in genetically engineered animals. The tumors were derived from 28 mice 828-1,489 days old, ofthree genotypes. Considerable cytological and architectural polymorphism was observed both within and between tumors. Most of the tumor cells were comparable in size to normal chromaffin cells or were larger. Small basophilic cells, which are the predominant cell type in rat pheochromocytomas, were usually in the minority. All of the tumors and most of the cells within individual tumors expressed immunoreactive tyrosine hydroxylase (TH). The tumors were variably positive for phenylethanolamine-N-methyltransferase (PNMT) and chromogranin A (CGA). There did not appear to be a global association of specific cytological features with expression of TH, PNMT, or CGA, although cells of similar appearance often shared similar immunoreactivities within individual tumors. Small basophilic cells could be either PNMT-positive or PNMT-negative. The frequency, morphology, and immunophenotype of mouse pheochromocytomas suggest that the mouse may be more appropriate than the rat as a model for human adrenal medullary pathology. In addition, the expression of immunoreactive PNMT by mouse pheochromocytomas suggests that these tumors are a potential source of epinephrine-producing cell lines, for which adequate models currently do not exist.

show abstract

Section: Discussionmentioning

confidence: 81%

Immunohistochemical and Morphological Characterization of Spontaneously Occurring Pheochromocytomas in the Aging Mouse

1996

View full text Add to dashboard Cite

show abstract

“…Several studies suggest that the development of adrenal medullary hyperplasia and pheochromocytomas in rats is accompanied by increasing NE/E ratios (Bär, 1988;Bosland and Bär, 1984;Eränkö , 1955;Ribelin et al, 1984 andTischler et al, 1985). Rat pheochromocytomas appear, in most instances, to have a distinctive phenotype characterized by predominant or exclusive production of NE and by secretory granules smaller and sparser than those in typical E and NE cells (Figure 8) (Tischler et al,Figure 7 Rat adrenal gland with 'malignant' pheochromocytoma.…”

Section: Functionmentioning

confidence: 99%

“…Catecholaminesynthesizing ability can almost invariably be demonstrated by other more sensitive methods (Bosland and Bär, 1984;Eränkö , 1955;Ribelin et al, 1984;Tischler et al, 1985 andTischler et al, 1990). However, this is no longer an issue.…”

Section: ) the Resemblance Of Tumor Cells To Immature Chromaffinmentioning

confidence: 99%

Toxic Responses of the Adrenal Medulla☆

Tischler¹,

Nyska²,

Elmore³

2014

Reference Module in Biomedical Sciences

View full text Add to dashboard Cite

“…Glucocorticoids (GCs) play an important role in stress and immunity (Griffin et al , ). As adrenal is the most sensitive endocrine organ to toxic insults, GC production becomes a target in adrenal toxicity (Ribelin et al , ). Studies have revealed a range of chemicals with the potential to cause adrenal toxicity altering the biosynthesis, secretion, transport, metabolism and binding of GCs (Harvey, ).…”

Section: Introductionmentioning

confidence: 99%

Molecular docking reveals the potential of phthalate esters to inhibit the enzymes of the glucocorticoid biosynthesis pathway

et al. 2016

View full text Add to dashboard Cite

Glucocorticoids (GCs) are well known to exert broad-based effects on metabolism, behavior and immunity. Their impaired synthesis and production lead to adverse health effects. Some environmental toxicants, including phthalate esters (PAEs), are associated with endocrine disruption. These endocrine-disrupting chemicals (EDCs) also cause adrenal toxicity and alteration of GC biosynthesis and their functions. Using in silico tools of Schrodinger Maestro 9.4, we performed a molecular docking study of 32 ligands including PAEs of a known endocrine-disrupting potential with the selected enzymes of the GC biosynthesis pathway (GBP) such as CYP11A1, CYP11B2, CYP19A1, CYP17A1, CYP21A2 and 3α/20β-HSD. Binding affinities of the PAEs were compared with known inhibitors of these enzymes. Amongst PAEs, diphenyl benzene-1, 2 - dicarboxylate (DPhP) showed the lowest docking score of -8.95616 kcal mol against CYP21A1. Besides, benzyl butyl benzene-1,2-dicarboxylate (BBzP), bis(7-methylnonyl) benzene-1,2 dicarboxylate (DIDP) and bis(2-ethylhexyl) benzene-1,2-dicarboxylate (DEHP) also showed comparable molecular interaction with enzymes of GBP. DPhP showed a significant molecular interaction with different enzymes of GBP such as CYP21A1, CYP11A1 and CYP11B2. These interactions mainly included H-bonding, hydrophobic, polar and van dar Waals' interactions. Interestingly, this in silico study revealed that certain PAEs have more inhibitory potential against enzymes of GBP than their respective known inhibitors. Such studies become more relevant in the risk assessment of exposure to mixtures of phthalate eaters. Copyright © 2016 John Wiley & Sons, Ltd.

show abstract

Minimally Functional Rat Adrenal Medullary Pheochromocytomas

Cited by 12 publications

References 7 publications

Immunohistochemical and Morphological Characterization of Spontaneously Occurring Pheochromocytomas in the Aging Mouse

Immunohistochemical and Morphological Characterization of Spontaneously Occurring Pheochromocytomas in the Aging Mouse

Toxic Responses of the Adrenal Medulla☆

Molecular docking reveals the potential of phthalate esters to inhibit the enzymes of the glucocorticoid biosynthesis pathway

Contact Info

Product

Resources

About