Minimal enhancer elements of the leghemoglobinlba andlbc3 gene promoters fromGlycine max L. have different properties

She, Qunxin; Lauridsen, Peter; Stougaard, Jens; Marcker, Kjeld A.

doi:10.1007/bf00028968

Cited by 13 publications

(12 citation statements)

References 34 publications

(79 reference statements)

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“…mants can be tested, making this system applicable for promoter studies. Furthermore, an analogous transformation system of Lotus corniculatus [8] has been successfully used for promoter studies of the leghemoglobin genes of soybean (Ibc3) [22,27,29], the soybean N23 gene [14,15] and the soybean ENOD2 gene [17]. The V. hirsuta hairy roots were inoculated with Rhizobium leguminosarum bv.…”

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A 200 bp region of the pea ENOD12 promoter is sufficient for nodule-specific and Nod factor induced expression

et al. 1995

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ENOD12 is one of the first nodulin genes expressed upon inoculation with Rhizobium and also purified Nod factors are able to induce ENOD12 expression. The ENOD12 gene family in pea (Pisum sativum) has two members. A cDNA clone representing PsENOD12A [26] and a PsENOD12B genomic clone [7] have been previously described. The isolation and characterization of a PsENOD12A genomic clone is presented in this paper. By using a Vicia hirsuta-Agrobacterium rhizogenes transformation system it is shown that both genes have a similar expression pattern in transgenic V. hirsuta root nodules. Promoter analyses of both PsENOD12 promoters showed that the 200 bp immediately upstream of the transcription start are sufficient to direct nodule-specific and Nod factor-induced gene expression.

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A 200 bp region of the pea ENOD12 promoter is sufficient for nodule-specific and Nod factor induced expression

et al. 1995

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“…Cis-regulatory promoter elements of nodulin genes have been identified in the ENOD2, Iba, Ibc3, and N23 genes from soybean, the Srglb3 gene from Sesbania rostrata, and the glutamine synthetase (gln-'~) gene from french bean [16,20,34,35,36,38,39,40].…”

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confidence: 99%

A novel type of DNA-binding protein interacts with a conserved sequence in an early nodulin ENOD12 promoter

et al. 1996

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The pea genes PsENOD12A and PsENOD12B are expressed in the root hairs shortly after infection with the nitrogen-fixing bacterium Rhizobium leguminosarum bv. viciae or after application of purified Nod factors. A 199 bp promoter fragment of the PsENOD12B gene contains sufficient information for Nod factor-induced tissue-specific expression. We have isolated a Vicia sativa cDNA encoding a 1641 amino acid protein, ENBP1, that interacts with the 199 bp ENOD12 promoter. Two different DNA-binding domains were identified in ENBP1. A domain containing six AT-hooks interacts specifically with an AT-rich sequence located between positions -95 and -77 in the PsENOD12B promoter. A second domain in ENBP1 is a cysteine-rich region that binds to the ENOD12 promoter in a sequence non-specific but metal-dependent way. ENBP1 is expressed in the same cell types as ENOD12. However, additional expression is observed in the nodule parenchyma and meristem. The presence of three small overlapping ORFs in the 5'-untranslated region of the ENBP1 cDNA indicates that ENBP1 expression might be regulated at the translational level. The interaction of ENBP1 with a conserved AT-rich element within the ENOD12 promoter and the presence of the ENBP1 transcript in cells expressing ENOD12 strongly suggest that ENBP1 is a transcription factor involved in the regulation of ENOD12. Finally, the C-terminal region of ENBP1 shows strong homology to a protein from rat that is specifically expressed in testis tissue.

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“…The regulation of late nodulin gene expression was studied in detail by analyzing different leghemoglobin (Ib) gene promoters from soybean and Sesbania rostrata [6,34,38,42,44]. Together, is analysis resulted in the identification of cis-acting elements conserved amongst different lb gene promoters [6,39].…”

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confidence: 99%

The promoter of the Vicia faba L. VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules

et al. 1995

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We recently reported on the broad bean gene VfENOD-GRP3 encoding a glycine-rich early nodulin. This gene was predominantly expressed in the interzone II-III region of Vicia faba root nodules. The VfENOD-GRP3 promoter contained several sequence motifs potentially involved in the regulation of gene expression. To investigate the molecular basis for the specific VfENOD-GRP3 expression, defined VfENOD-GRP3 promoter fragments were fused to an intron-containing gusAint gene. Agrobacterium rhizogenes ARqual strains carrying these fusions integrated into the TL DNA were used to generate hairy roots on Vicia hirsuta, which subsequently were nodulated. Histochemical analysis of transgenic nodules indicated that a strong gusAint expression in the interzone II-III region was mediated by the -1252/+10 VfENOD-GRP3 promoter region. This reporter gene expression in V. hirsuta was comparable to the location of VfENOD-GRP3 transcripts in V. faba nodules. An analysis of defined promoter fragments revealed that a strong gusAint expression in the interzone II-III region was also mediated by the -737/+10 promoter, whereas the -239/+10 promoter only mediated a weak gusAint expression in the interzone II-III region. Since the -239/+10 promoter fragment did not resemble published nodulin gene promoters, we propose that it contains new sequence motifs involved in mediating gene expression in the interzone II-III region of Vicia nodules.

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Minimal enhancer elements of the leghemoglobinlba andlbc3 gene promoters fromGlycine max L. have different properties

Cited by 13 publications

References 34 publications

A 200 bp region of the pea ENOD12 promoter is sufficient for nodule-specific and Nod factor induced expression

A 200 bp region of the pea ENOD12 promoter is sufficient for nodule-specific and Nod factor induced expression

A novel type of DNA-binding protein interacts with a conserved sequence in an early nodulin ENOD12 promoter

The promoter of the Vicia faba L. VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules

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