Miniaturized System with a Facile Isothermal Amplification Microfluidic Chip for Rapid Detection of Zika Viruses

Abstract

Abstract.A real-time fluorescence detection biomedical miniaturized system with a disposable microfluidic chip was developed for loop-mediated isothermal amplification (LAMP) reactions. The miniaturized system was developed consisting of a mini heating plate, a temperature sensor, a temperature controller and an adjustable mechanical stage. Based on experimental result, the temperature of the heating plate is evenly distributed, and the error of the temperature is less than 1.3%. The microfluidic chip was designed and fabricated by MEMS technology and thin-casting method. The volume of the LAMP reaction was changed from 25 μL to 2 μL. The volume of the LAMP reaction was optimized. The nucleic acid of Zika viruses was amplified and detected in real-time mode with our miniatureized system by using above microfluidic chip. From the experiment results, the detection range was from 100 copies to 10 6 copies. This biomedical system has the potential for point-of-care diagnostics (POCT) with the many advantages, such as not high cost, short analysis and detection time, not much reagent and sample consumption and so on.