Miniaturized probe based on a microelectromechanical system mirror for multiphoton microscopy

Jung, Woonggyu; Tang, Suo; McCormic, Daniel T.; Xie, Tiquiang; Ahn, Yeh‐Chan; Su, Jianping; Tomov, I. V.; Krasieva, Tatiana B.; Tromberg, Bruce J.; Chen, Zhongping

doi:10.1364/ol.33.001324

Cited by 72 publications

(62 citation statements)

References 12 publications

(18 reference statements)

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“…The development of NLOM probe based on multiphoton excitation that uses a near infrared laser beam for imaging is in progress by several groups [20][21][22]. An important factor that limits the use of NLOM probe in clinical studies is the lack of a compactness and flexibility.…”

Section: Introductionmentioning

confidence: 99%

“…An important factor that limits the use of NLOM probe in clinical studies is the lack of a compactness and flexibility. Jung et al, reported a miniaturized multiphoton microscopy probe that was developed by employing a microelectromechanical system scanning mirror and a double-clad photonic crystal fiber [20]. The size of probe head was 1 cm in outer diameter and 14 cm in length [20].…”

Section: Introductionmentioning

confidence: 99%

“…Jung et al, reported a miniaturized multiphoton microscopy probe that was developed by employing a microelectromechanical system scanning mirror and a double-clad photonic crystal fiber [20]. The size of probe head was 1 cm in outer diameter and 14 cm in length [20]. As another development, a miniaturized probe that possesses a diameter of 0.4 mm was developed by Bao et al, for two-photon-excited fluorescence imaging [22].…”

Section: Introductionmentioning

confidence: 99%

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Nonlinear optical microscopy of early stage (ICRS Grade-I) osteoarthritic human cartilage

Kumar

Grønhaug

Davies

et al. 2015

Biomed. Opt. Express

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Abstract:In a synovial joint, the articular cartilage is directly affected during the progression of Osteoarthritis (OA). The characterization of early stage modification in extra-cellular matrix of cartilage is essential for detection as well as understanding the progression of disease. The objective of this study is to demonstrate the potential and capability of nonlinear optical microscopy for the morphological investigation of early stage osteoarthritic cartilage. ICRS Grade-I cartilage sections were obtained from the femoral condyle of the human knee. The surface of articular cartilage was imaged by second harmonic generation and two-photon excited fluorescence microscopy. Novel morphological features like microsplits and wrinkles were observed, which would otherwise not be visible in other clinical imaging modalities (e.g., CT, MRI, ultrasound and arthroscope. The presence of superficial layer with distinct collagen fibrils parallel to the articular surface in 4 specimens out of 14 specimens, indicates that different phases of OA within ICRS Grade-I can be detected by SHG microscopy. All together, the observed novel morphologies in early stage osteoarthritic cartilage indicates that SHG microscopy might be a significant tool for the assessment of cartilage disorder.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nonlinear optical microscopy of early stage (ICRS Grade-I) osteoarthritic human cartilage

Kumar

Grønhaug

Davies

et al. 2015

Biomed. Opt. Express

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“…The compact circuit board is designed by AdvancedMEMS, Inc. to guarantee a miniaturized probe with the ability to image tissue in vivo [40,41]. V grooves included in the probe can help to adjust the positional tolerance in XYZ decenter, Theta, and Phi-tilt of both the MEMS device and collimator component.…”

Section: Multimodal Nonlinear Endo-microscopy Probe Schemementioning

confidence: 99%

Multimodal nonlinear endo-microscopy probe design for high resolution, label-free intraoperative imaging

McCormick³

et al. 2015

Biomed. Opt. Express

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We present a portable, multimodal, nonlinear endo-microscopy probe designed for intraoperative oncological imaging. Application of a four-wave mixing noise suppression scheme using dual wavelength wave plates (DWW) and a polarization-maintaining fiber improves tissue signal collection efficiency, allowing for miniaturization. The probe, with a small 14 mm transversal diameter, includes a customized miniaturized two-axis MEMS (micro-electromechanical system) raster scanning mirror and microoptics with an illumination laser delivered by a polarization-maintaining fiber. The probe can potentially be integrated into the arms of a surgical robot, such as da Vinci robotic surgery system, due to its minimal cross sectional area. It has the ability to incorporate multiple imaging modalities including CARS (coherent anti-Stokes Raman scattering), SHG (second harmonic generation), and TPEF (two-photon excited fluorescence) in order to allow the surgeon to locate tumor cells within the context of normal stromal tissue. The resolution of the endo-microscope is experimentally determined to be 0.78 µm, a high level of accuracy for such a compact probe setup. The expected resolution of the as-built multimodal, nonlinear, endo-microscopy probe is 1 µm based on the calculation tolerance allocation using Monte-Carlo simulation. The reported probe is intended for use in laparoscopic or radical prostatectomy, including detection of tumor margins and avoidance of nerve impairment during surgery. "Label-free high-resolution imaging of prostate glands and cavernous nerves using coherent anti-Stokes Raman scattering microscopy," Biomed. Opt. Express 2(4), 915-926 (2011). 44. X. Xu, J. Cheng, M. J. Thrall, Z. Liu, X. Wang, and S. T. C. Wong, "Multimodal non-linear optical imaging for label-free differentiation of lung cancerous lesions from normal and desmoplastic tissues," Biomed. Opt. Express 4(12), 2855-2868 (2013).

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“…Recent advances in this direction include development of fiber-based compact probes which are being used successfully in optical imaging techniques based on optical coherence tomography 1 , two-photon fluorescence (TPEF) and second harmonic generation (SHG) 2,3 . Among the optical imaging methods currently used, the development of coherent anti-Stokes Raman Scattering (CARS) remains at a level much closer to the laboratory bench than to the clinic.…”

Section: Introductionmentioning

confidence: 99%

Scheme for efficient fiber-based CARS probe

et al. 2010

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Abstract. We demonstrate a fiber-based probe for maximum collection of the Coherent anti-Stokes Raman Scattering (CARS) signal in biological tissues. We discuss the design challenges including capturing the back-scattered forward generated CARS signal in the sample and the effects of fiber nonlinearities on the propagating pulses. Three different biological tissues were imaged in vitro in order to assess the performance of our fiberdelivered probe for CARS imaging, a tool which we consider an important advance towards label-free, in vivo probing of superficial tissues.

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Miniaturized probe based on a microelectromechanical system mirror for multiphoton microscopy

Cited by 72 publications

References 12 publications

Nonlinear optical microscopy of early stage (ICRS Grade-I) osteoarthritic human cartilage

Nonlinear optical microscopy of early stage (ICRS Grade-I) osteoarthritic human cartilage

Multimodal nonlinear endo-microscopy probe design for high resolution, label-free intraoperative imaging

Scheme for efficient fiber-based CARS probe

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