Miniaturized PCR-Chip for Dna Amplification With an External Peristaltic Pump

Abstract

Since 1985, the polymerse chain reaction (PCR) became very popular among the field of molecular biology. It is very powerful for amplification DNA segment and it had a variety of applications in medicinal, virus, disease, and monitoring. In this study, a simple and miniature PCR chip will be presented with an external peristaltic pump utilized to driving liquid into PCR chip. 1 The PCR chip was made of PMMA (Polymethylmethacrylate) and glass. The hot embossing technique was used to fabricate the micro channel on the PMMA. The copper was sputtered on the glass as the heater. The glass and PMMA were bonded by PDMS. In typically, heater temperature was 94 • C for denature, 55 • C for annealing, 72 • C for extension. Therefore, the heaters formed three different zones of temperature along the channel that the length ration on each temperature zone was 1:1:2 for denature, annealing and extension, respectively. 2,3 For temperature control, the PID control mode was used to regulate the temperature on reaction and the DC power was as the power supplier. The thermal sensors were adhered on the heater beside the channel.

Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends

“…During the past two years, the miniaturization of such PCR devices has been of growing interest. In general, they can be classified as single chamber stationary PCR chip ( Figure 1A) (10,(12)(13)(14)(15)(16)18,21,22,24,26,27,29,32,37,39,40,43,45,46,48,(51)(52)(53)57,62,63,(68)(69)(70) and multi chamber stationary PCR chip ( Figure 1B) (11,17,19,20,25,28,31,(33)(34)(35)(36)38,47,49,54,78). The former can perform very well in terms of fluidic and thermal controls and offer beneficial properties such as reduced thermal and fluidic crosstalk between chambers.…”

PMMA biosensor for nucleic acids with integrated mixer and electrochemical detection

“…PMMA is a popular substrate due to its machinability, low cost and optical clarity (Castaño-Álvarez et al, 2005;Chen et al, 2003;Li et al, 2005a,b;Liu et al, 2004;Muck et al, 2004;Qi et al, 2002;Wei et al, 2005;Yu and Chien, 2005). Microchannels are commonly formed in PMMA using laser ablation (Klank et al, 2002;Yao et al, 2005) and hot embossing (Brown et al, 2006;Grass et al, 2001;Lee et al, 2001;Li et al, 2005a,b;Soper et al, 2005).…”