Miniaturized Paper-Based Gene Sensor for Rapid and Sensitive Identification of Contagious Plant Virus

Wei, Jitao; Liu, Hongxing; Liu, Fang; Zhu, Minjun; Zhou, Xiaoming; Xing, Da

doi:10.1021/am506695g

Cited by 32 publications

(14 citation statements)

References 51 publications

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“…Au NPs have shown great promise in the facile, on-site detection of contagious plant viruses when applied to a strip sensor (Zhao et al, 2011; Wei et al, 2014). These nanoparticles can be functionalized to target specific sites within an organism's cellular substructure, and could allow genetic modifications to be made from within the plant itself, rather than through a traditional in-vitro process.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Understanding of Nanoparticle Uptake in Watermelon Plants

et al. 2016

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The use of agrochemical-nutrient fertilizers has come under scrutiny in recent years due to concerns that they damage the ecosystem and endanger public health. Nanotechnology offers many possible interventions to mitigate these risks by use of nanofertilizers, nanopesticides, and nanosensors; and concurrently increases profitability, yields, and sustainability within the agricultural industry. Aerosol based foliar delivery of nanoparticles may help to enhance nanoparticle uptake and reduce environmental impacts of chemical fertilizers conventionally applied through a soil route. The purpose of this work was to study uptake, translocation, and accumulation of various gold nanostructures, 30–80 nm, delivered by aerosol application to a watermelon plant. Cellular uptake and accumulation of gold nanoparticles were quantified by Inductively Coupled Plasma-Mass Spectroscopy (ICP-MS). Observations suggested that nanoparticles could be taken up by the plant through direct penetration and transport through the stomatal opening. Observed translocation of nanoparticles from leaf to root shows evidence that nanoparticles travel by the phloem transport mechanism. Accumulation and transport of nanoparticles depend on nanoparticle shape, application method, and nature of plant tissues.

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Section: Introductionmentioning

confidence: 99%

Quantitative Understanding of Nanoparticle Uptake in Watermelon Plants

et al. 2016

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“…Many human pathogens such as Mycobacterium tuberculosis, Leishmania spp., and E. coli and plant pathogens such as tomato leaf curl virus have been developed with functionalized AuNPs (Baptista et al 2006;Soo et al 2009;Padmavathy et al 2012;Andreadou et al 2014;Larguinho et al 2015;Dharanivasan et al 2016). The paper-based gene sensor was developed for the detection of BBTV using a probe-conjugated AuNP (Wei et al 2014). Though this method could detect BTV DNA up to (0.13 aM), prior amplification of BBTV by PCR is needed.…”

Section: Introductionmentioning

confidence: 99%

Cloning of BBTV (Banana Bunchy Top Virus) components and screening of BBTV using functionalized gold nanoparticles

2017

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Banana bunchy top virus (BBTV) affects all varieties of banana plants and causes heavy economic loss in most of the banana cultivating areas. The BBTV genome comprises of six DNA components; in this study, we have cloned the six BBTV-DNA components from one of the BBTV-infected plants (Tri-8) and were submitted to GenBank. Analysis of the BBTV DNA-R component showed that it belonged to south Pacific group. Resistance against BBTV has not been observed so far in banana plants and removal and killing of the infected plants has been routinely practiced. Hence, early detection of BBTV infection would be desirable and various detection methods routinely employed include enzyme linked immunosorbent assay (antigen-antibody based) and molecular-based methods such as polymerase chain reaction (PCR), qPCR, or LAMP PCR. Most of these methods require enzymes or antibodies for detection and hence are expensive. Here, we report a visual detection method (AuNP probe assay) using gold nanoparticles (AuNPs) functionalized with an ssDNA-thiolated probe (CR1). This method is based on the hybridization of the functionalized AuNPs with the target DNA (BBTV). In the AuNP probe assay, the functionalized AuNPs retains red colour when BBTV DNA is present, and in the absence of BBTV DNA, the colour of the functionalized AuNPs changes to purple when salt is added. The AuNP probe assay was compared with PCR for the detection of banana plants and it was found that AuNP probe assay was better than PCR in detecting BBTV infection (86.5% for AuNP probe assay and 65% for PCR). The AuNP probe assay was found to be highly specific to BBTV and was found to detect up to 1 pg/μl of the plasmid (pTZBBTri 4, BBTV DNA) mixed with healthy banana DNA.

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“…Due to the advantages of simplicity, ability of capillary action and sample storage, many paper-based colorimetric analysis platforms have been built to detect nucleic acid, protein, etc. [95,96]. Benefiting from the advantages of malleability, foldability, as well as the ability to filter the sample, several paper-based platforms integrate purification of sample, nucleic acid amplification and detection [97,98,99].…”

Section: Electrochemiluminescence (Ecl)-based Detection Of Pathogenmentioning

confidence: 99%

Recent Advances in Electrochemiluminescence Sensors for Pathogenic Bacteria Detection

2019

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Pathogenic bacterial contamination greatly threats human health and safety. Rapidly biosensing pathogens in the early stage of infection would be helpful to choose the correct drug treatment, prevent transmission of pathogens, as well as decrease mortality and economic losses. Traditional techniques, such as polymerase chain reaction and enzyme-linked immunosorbent assay, are accurate and effective, but are greatly limited because they are complex and time-consuming. Electrochemiluminescence (ECL) biosensors combine the advantages of both electrochemical and photoluminescence analysis and are suitable for high sensitivity and simple pathogenic bacteria detection. In this review, we summarize recent advances in ECL sensors for pathogenic bacteria detection and highlight the development of paper-based ECL platforms in point of care diagnosis of pathogens.

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Miniaturized Paper-Based Gene Sensor for Rapid and Sensitive Identification of Contagious Plant Virus

Cited by 32 publications

References 51 publications

Quantitative Understanding of Nanoparticle Uptake in Watermelon Plants

Quantitative Understanding of Nanoparticle Uptake in Watermelon Plants

Cloning of BBTV (Banana Bunchy Top Virus) components and screening of BBTV using functionalized gold nanoparticles

Recent Advances in Electrochemiluminescence Sensors for Pathogenic Bacteria Detection

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