Miniaturized microarray-format digital ELISA enabled by lithographic protein patterning

Stephens, Amanda J.; Song, Yujing; McClellan, Brandon L.; Su, Shiuan‐Haur; Xu, Sonnet; Chen, K.; Castro, Maria G.; Singer, Benjamin H.; Kurabayashi, Katsuo

doi:10.1016/j.bios.2023.115536

Cited by 7 publications

(3 citation statements)

References 34 publications

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“…The lateral array number and bead color allowed the simultaneous detection of 12-plex interleukins in total. Stephens et al 81 printed six different types of capture antibodies on the different zones of the microwell surface to simultaneously measure six serum secretory factors related to glioma tumor progression in a cohort of mice. Since this digital protein microarray method eliminates the use of microbeads from the assay, there is no need to spend an extended amount of time on taking sufficient images of each kind of beads corresponding to each analyte.…”

Section: Multiplexingmentioning

confidence: 99%

Recent progress in digital immunoassay: how to achieve ultrasensitive, multiplex and clinical accessible detection?

Zhang,

Gu,

2024

Sens. Diagn.

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Section: Multiplexingmentioning

confidence: 99%

Recent progress in digital immunoassay: how to achieve ultrasensitive, multiplex and clinical accessible detection?

Zhang,

Gu,

2024

Sens. Diagn.

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“…The development of functional biosensors has long drawn considerable research interests across many different disciplines in fundamental science, biotechnology, and medicine [1][2][3][4][5][6][7][8][9][10][11]. One of the notable trends in recent efforts for biosensor development involves high miniaturization [6,8,[12][13][14] and flexibility/wearability [3,5,7,9,15,16]. Advances in nanoscience continuously propel such a drive to create flexible and miniaturized biosensors, permitting high-throughput detection of bioanalytes that are held on an array of nanometer-sized sensor surfaces in a flexible setting.…”

Section: Introductionmentioning

confidence: 99%

“…Advances in nanoscience continuously propel such a drive to create flexible and miniaturized biosensors, permitting high-throughput detection of bioanalytes that are held on an array of nanometer-sized sensor surfaces in a flexible setting. The majority of conventional biosensors are fabricated by top-down approaches such as photolithography, soft (microcontact printing) lithography, and inkjet printing, which can be costly and time-consuming [2,13,[17][18][19][20][21][22][23][24][25]. Fabrication techniques relying on conventional lithographic procedures also present limitations in the size of the smallest possible sensor unit that can be individually addressed.…”

Section: Introductionmentioning

confidence: 99%

Self-Assembled Block Copolymers as a Facile Pathway to Create Functional Nanobiosensor and Nanobiomaterial Surfaces

Sytu,

Cho,

Hahm

2024

Polymers

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Block copolymer (BCP) surfaces permit an exquisite level of nanoscale control in biomolecular assemblies solely based on self-assembly. Owing to this, BCP-based biomolecular assembly represents a much-needed, new paradigm for creating nanobiosensors and nanobiomaterials without the need for costly and time-consuming fabrication steps. Research endeavors in the BCP nanobiotechnology field have led to stimulating results that can promote our current understanding of biomolecular interactions at a solid interface to the never-explored size regimes comparable to individual biomolecules. Encouraging research outcomes have also been reported for the stability and activity of biomolecules bound on BCP thin film surfaces. A wide range of single and multicomponent biomolecules and BCP systems has been assessed to substantiate the potential utility in practical applications as next-generation nanobiosensors, nanobiodevices, and biomaterials. To this end, this Review highlights pioneering research efforts made in the BCP nanobiotechnology area. The discussions will be focused on those works particularly pertaining to nanoscale surface assembly of functional biomolecules, biomolecular interaction properties unique to nanoscale polymer interfaces, functionality of nanoscale surface-bound biomolecules, and specific examples in biosensing. Systems involving the incorporation of biomolecules as one of the blocks in BCPs, i.e., DNA–BCP hybrids, protein–BCP conjugates, and isolated BCP micelles of bioligand carriers used in drug delivery, are outside of the scope of this Review. Looking ahead, there awaits plenty of exciting research opportunities to advance the research field of BCP nanobiotechnology by capitalizing on the fundamental groundwork laid so far for the biomolecular interactions on BCP surfaces. In order to better guide the path forward, key fundamental questions yet to be addressed by the field are identified. In addition, future research directions of BCP nanobiotechnology are contemplated in the concluding section of this Review.

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Biologically Active Micropatterns of Biomolecules and Living Matter Using Microbubble Lithography

Ranjan,

Bhowmick,

Gupta

et al. 2024

Small

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In situ patterning of biomolecules and living organisms while retaining their biological activity is extremely challenging, primarily because such patterning typically involves thermal stresses that could be substantially higher than the physiological thermal or stress tolerance level. Top‐down patterning approaches are especially prone to these issues, while bottom‐up approaches suffer from a lack of control in developing defined structures and the time required for patterning. A microbubble generated and manipulated by optical tweezers (microbubble lithography) is used to self‐assemble and pattern living organisms in continuous microscopic structures in real‐time, where the material thus patterned remains biologically active due to their ability to withstand elevated temperatures for short exposures. Successful patterns of microorganisms (Escherichia coli, Lactococcus. lactis and the Type A influenza virus) are demonstrated, as well as reporter proteins such as green fluorescent protein (GFP) on functionalized substrates with high signal‐to‐noise ratio and selectivity. Together, the data presented herein may open up fascinating possibilities in rapid in situ parallelized diagnostics of multiple pathogens and bioelectronics.

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Miniaturized microarray-format digital ELISA enabled by lithographic protein patterning

Cited by 7 publications

References 34 publications

Recent progress in digital immunoassay: how to achieve ultrasensitive, multiplex and clinical accessible detection?

Recent progress in digital immunoassay: how to achieve ultrasensitive, multiplex and clinical accessible detection?

Self-Assembled Block Copolymers as a Facile Pathway to Create Functional Nanobiosensor and Nanobiomaterial Surfaces

Biologically Active Micropatterns of Biomolecules and Living Matter Using Microbubble Lithography

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