Miniaturized liquid chromatography focusing on analytical columns and mass spectrometry: A review

Maciel, Edvaldo Vasconcelos Soares; Toffoli, Ana Lúcia de; Sobieski, Eduardo; Nazário, Carlos Eduardo Domingues; Lanças, Fernando Mauro

doi:10.1016/j.aca.2019.12.064

Cited by 93 publications

(32 citation statements)

References 212 publications

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“…The development of an HPLC column with an "ideal and perfect" peptide/protein separation capability is an important, continuous research objective. The main types of analytical columns currently used in proteomics research present different characteristics with regard to the material composition and particle size packed into it, and the length and diameter [92]. Remarkable separation of complex peptide mixtures in proteomic studies was achieved using nano-LC/UPLC and capillary columns containing packed alkyl bonded C8 or C18 [93] and silica-based monolithic capillaries [94,95].…”

Section: The Goodmentioning

confidence: 99%

A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of This Field

et al. 2020

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Proteomics is the field of study that includes the analysis of proteins, from either a basic science prospective or a clinical one. Proteins can be investigated for their abundance, variety of proteoforms due to post-translational modifications (PTMs), and their stable or transient protein–protein interactions. This can be especially beneficial in the clinical setting when studying proteins involved in different diseases and conditions. Here, we aim to describe a bottom-up proteomics workflow from sample preparation to data analysis, including all of its benefits and pitfalls. We also describe potential improvements in this type of proteomics workflow for the future.

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Section: The Goodmentioning

confidence: 99%

A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of This Field

et al. 2020

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“…Capillary and nano-liquid chromatography (CLC and nano-LC) provide various advantages over conventional HPLC, such as the wide reduction in mobile and stationary phase consumption, including toxic reagents; the small sample volume needed; high separation efficiency, the easy coupling to mass spectrometry (MS). In addition, the reduction of particle diameter and column length allows rapid analyses [25,26]. Currently, one of the most important advantages is the reduction of waste generation, in accordance with the principles of green chemistry [27].…”

Section: Introductionmentioning

confidence: 99%

A Rapid Nano-Liquid Chromatographic Method for the Analysis of Cannabinoids in Cannabis sativa L. Extracts

Žampachová

Aturki²,

Mariani³

et al. 2021

Molecules

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Cannabis sativa L. is an herbaceous plant belonging to the family of Cannabaceae. It is classified into three different chemotypes based on the different cannabinoids profile. In particular, fiber-type cannabis (hemp) is rich in cannabidiol (CBD) content. In the present work, a rapid nano liquid chromatographic method (nano-LC) was proposed for the determination of the main cannabinoids in Cannabis sativa L. (hemp) inflorescences belonging to different varieties. The nano-LC experiments were carried out in a 100 µm internal diameter capillary column packed with a C18 stationary phase for 15 cm with a mobile phase composed of ACN/H2O/formic acid, 80/19/1% (v/v/v). The reverse-phase nano-LC method allowed the complete separation of four standard cannabinoids in less than 12 min under isocratic elution mode. The nano-LC method coupled to ultraviolet (UV) detection was validated and applied to the quantification of the target analytes in cannabis extracts. The nano-LC system was also coupled to an electrospray ionization–mass spectrometry (ESI-MS) detector to confirm the identity of the cannabinoids present in hemp samples. For the extraction of the cannabinoids, three different approaches, including dynamic maceration (DM), ultrasound-assisted extraction (UAE), and an extraction procedure adapted from the French Pharmacopeia’s protocol on medicinal plants, were carried out, and the results achieved were compared.

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“…reproducible detection of 19,000 m/z features in a total run time of 0.45 min. LC miniaturization strategies also offer potential for increased (cost) efficiency [69]. However, the challenges for miniaturisation of sampling/extraction of plant materials, compared with plasma or urine, cannot be underestimated.…”

Section: Open Accessmentioning

confidence: 99%

High-throughput plant phenotyping: a role for metabolomics?

Hall

D’Auria

Ferreira

et al. 2022

Trends in Plant Science

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Miniaturized liquid chromatography focusing on analytical columns and mass spectrometry: A review

Cited by 93 publications

References 212 publications

A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of This Field

A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of This Field

A Rapid Nano-Liquid Chromatographic Method for the Analysis of Cannabinoids in Cannabis sativa L. Extracts

High-throughput plant phenotyping: a role for metabolomics?

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