Miniaturized FISH for Screening of Onco-Hematological Malignancies

Zanardi, Andrea; Bandiera, Dario; Corsini, Chiara; Gregato, Giuliana; Milani, P.; Barborini, E.; Carbone, Roberta

doi:10.2144/000113445

Cited by 39 publications

(36 citation statements)

References 21 publications

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“…Liu et al 13 reported modification of a glass surface with (3-aminopropyl) triethoxysilane (APTES) and polyethylene glycol (PEG)-silane for fabrication of a cell array. To promote cell attachment, Zanardi et al 14 performed surface coating with TiO2 nanoparticles.…”

Section: ·2 Materialsmentioning

confidence: 99%

Microdevice in Cellular Pathology: Microfluidic Platforms for Fluorescence in situ Hybridization and Analysis of Circulating Tumor Cells

Sato

2015

ANAL. SCI.

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867 IntroductionCellular pathology techniques have progressed in recent years. Conventionally, the pathologist observes tissues with a microscope and determines whether or not the hematoxylin and eosin (H&E)-stained tissues contain cancer cells based on their morphology. The H&E stain is the traditional and most widely used staining method in medical diagnoses. 1 Recent advances in genetic analysis methods have made it possible to identify the genetic basis of human diseases, and have opened the door to individualized prevention strategies and early detection and treatment.Therefore, combinations of the traditional morphological diagnosis method and newer genetic methodologies are strongly welcomed.Fluorescence in situ hybridization (FISH) is a well-known gene-based method used to image genetic abnormalities. FISH is a microscopic technique in which specific DNA sequences tagged with fluorophores are used to detect target genes and identify their localization within a cell. FISH was developed in the early 1980s 2 and has since been improved continuously. 3 The FISH probes can only detect genes with a high degree of homology, and can visualize specific cytogenetic abnormalities such as copy number aberrations, gene mutations, and gene fusions. 4 This advanced molecular pathology technique has enabled better diagnoses of diseases, leading to more tailored therapeutic regimens.Analytes have become more multifaceted. Microfluidic devices enable the miniaturization, integration, automation, and parallelization of chemical and biochemical processes. This new technology also provides opportunity for expansion in the field of cellular pathology. Fluorescence in situ hybridization (FISH) is a well-known gene-based method to image genetic abnormalities. Development of a FISH microfluidic platform has offered the possibility of automation with significant time and cost reductions, which overcomes many drawbacks of the current protocols. Microfluidic devices are also powerful tools for single-cell analysis. Capturing the circulating tumor cells (CTCs) from blood samples is one of the most promising approaches to enable the early diagnosis of cancer. The microfluidic devices are also useful to isolate rare CTCs at high efficiency and purity. In this review, I outline recent FISH and CTC analyses using microfluidic devices, and describe their applications for the cellular diagnosis of cancers.

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Section: ·2 Materialsmentioning

confidence: 99%

Microdevice in Cellular Pathology: Microfluidic Platforms for Fluorescence in situ Hybridization and Analysis of Circulating Tumor Cells

Sato

2015

ANAL. SCI.

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“…The most recent example of an interphase FISH on chip was presented by Zanardi et al, (2010). They presented a miniaturization of the interphase FISH protocol through microfluidic methods with minimized shear stress exerted on cells (living or fixed) during sample loading.…”

Section: Miniaturized Interphase Fishmentioning

confidence: 99%

Microtechnologies Enable Cytogenetics

Kwasny¹,

Vedarethinam²,

Shah³

et al. 2012

Recent Trends in Cytogenetic Studies - Methodologies and Applications

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“…There has been few reports on performing cytogenetic analysis using microfabricated devices [6][7][8][9][10][11][12][13][14][15]. They offer a reduction in the reagents volume with great focus on minimizing the amount of the DNA probe used.…”

Section: Introductionmentioning

confidence: 99%

A Semi-Closed Device for Chromosome Spreading for Cytogenetic Analysis

et al. 2014

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Metaphase chromosome spreading is the most crucial step required for successful karyotyping and FISH analysis. These two techniques are routinely used in cytogenetics to assess the chromosome abnormalities. The spreading process has been studied for years but it is still considered an art more than a science. The chromosome spreading greatly depends on the environmental conditions such as humidity and temperature, which govern the evaporation of fixative, in which the cells are suspended. The spreading is normally performed manually in ambient conditions on glass slides, which are hydrophilic, and thus allow for better quality spreads. Further cytogenetic analysis depends on the quality of the spreads, which is dependent on the skills of the personnel and is thus limited to laboratory settings. Here, we present a semi-closed microfluidic chip for preparation of the metaphase spreads on a glass and a Topas substrate rendered more hydrophilic by oxygen plasma treatment coupled with photografting. The device consists of a microfluidic chamber with perfusion holes that facilitate the evaporation of fixative and reliable formation of the spreads.Micromachines 2014, 5 159The usability of the chromosome spreads formed on the glass and the Topas slide is tested by performing FISH analysis.

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Miniaturized FISH for Screening of Onco-Hematological Malignancies

Cited by 39 publications

References 21 publications

Microdevice in Cellular Pathology: Microfluidic Platforms for Fluorescence in situ Hybridization and Analysis of Circulating Tumor Cells

Microdevice in Cellular Pathology: Microfluidic Platforms for Fluorescence in situ Hybridization and Analysis of Circulating Tumor Cells

Microtechnologies Enable Cytogenetics

A Semi-Closed Device for Chromosome Spreading for Cytogenetic Analysis

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