Miniaturized Electrophoresis: An Evolving Role in Laboratory Medicine

Jin, Lian; Ferrance, Jerome P.; Landers, James P.

doi:10.2144/01316md01

Cited by 36 publications

(26 citation statements)

References 81 publications

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“…Electrophoresis is well known and has found many applications in medicine and materials [205][206][207]. Electrophoresis is the movement of particles, with a fixed surface charge, in a solvent by an electric field gradient [179].…”

Section: Carbon Encapsulated Nanoparticlesmentioning

confidence: 99%

Template synthesis of nanomaterials

Wade

Wegrowe

2005

Eur. Phys. J. Appl. Phys.

125

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Abstract. We present an overview of template synthesis as it applies to our nanomaterials research. This bottom-up approach is motivated by our desire to find an alternative to the big, top-down approaches to nanoscience, such as clean-rooms and X-ray lithography. Using universally available templates and materials, and very modest synthesis techniques, we have created a variety of interesting and useful structures. Starting with homogeneous ferromagnetic nanowires, we were able to study and manipulate spin-dependent transport. Next, we branched into multi-layer GMR and spin-valve structures for spintronics. As a side trip, we put carbon-encapsulated fullerene nanoparticles into nanopores for ballistic magnetoresistance studies. Carbon nanotube molecules were grown in templates by CVD self assembly. The carbon nanotubes grown using a cobalt catalyzer show spin-valve, ballistic transport, and Coulomb blockade effects. Very recently, we have started to study templated semiconductor nanorods with the amazing result that their behaviour is very similar to that of the carbon nanotubes and can be reduced to a scaling law. Essentially, the template acts as a skeleton for the nanoscale synthesis and macroscale contact of an infinite variety of materials and structures. It is our hope that by the following examples we demonstrate that high quality nanoscience research is available to everybody. PACS

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Section: Carbon Encapsulated Nanoparticlesmentioning

confidence: 99%

Template synthesis of nanomaterials

Wade

Wegrowe

2005

Eur. Phys. J. Appl. Phys.

125

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“…A variety of electrical, optical, and biochemical approaches have been explored for each of these stages. However, devices fully integrating all four steps are scarce, and those that exist, although innovative and ingenious, are only the beginning steps in a field with boundless opportunities (29,30).…”

Section: Enabling Chip Technologiesmentioning

confidence: 99%

Designing a nano-interface in a microfluidic chip to probe living cells: Challenges and perspectives

Helmke

Minerick

2006

Proc. Natl. Acad. Sci. U.S.A.

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Nanotechnology-based materials are beginning to emerge as promising platforms for biomedical analysis, but measurement and control at the cell-chip interface remain challenging. This idea served as the basis for discussion in a focus group at the recent National Academies Keck Futures Initiative. In this Perspective, we first outline recent advances and limitations in measuring nanoscale mechanical, biochemical, and electrical interactions at the interface between biomaterials and living cells. Second, we present emerging experimental and conceptual platforms for probing living cells with nanotechnology-based tools in a microfluidic chip. Finally, we explore future directions and critical needs for engineering the cell-chip interface to create an integrated system capable of highresolution analysis and control of cellular physiology.

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“…More recently [25], we combined HA and restriction-length polymorphism analysis, (i.e., sizing and sequence-specific enzymatic cleavage of DNA) to increase the sensitivity of the method. Jin et al [26] and Andersen et al [27] have also presented reviews of microchip-based mutation detection methods.…”

Section: Microchip-based Mutation Detectionmentioning

confidence: 99%

An integrated method for mutation detection using on‐chip sample preparation, single‐stranded conformation polymorphism, and heteroduplex analysis

Vahedi¹,

Kaler²,

Backhouse³

2004

Electrophoresis

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This work integrates rapid techniques for mutation detection by producing single-stranded DNA and (renatured) double-stranded DNA on-chip, labeling these with fluorescent DNA stains and then performing two complementary methods of mutation detection-single stranded conformation polymorphism (SSCP) analysis and heteroduplex analysis (HA). This involves the denaturation of double-stranded polymerase chain reaction (PCR) product into single-stranded DNA, the mutation analysis of the single-stranded DNA by SSCP and the rehybridized double-stranded DNA by HA. These steps were performed entirely on-chip within several minutes of operation. The combination of these two mutation detection methods on-chip provides a highly sensitive method of mutation detection for either genotyping or screening. Many mutation analysis methods rely upon fluorescently labeled samples from a PCR with fluorescently labeled primers. By labeling on-chip we not only attain improved signal strength, but the method is considerably more versatile. Although we used PCR products in this work, this method could be used to analyze DNA from any source. We believe that this combination of several procedures on a single chip represents a significant step in the development of higher levels of integration upon microfluidic devices.

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Miniaturized Electrophoresis: An Evolving Role in Laboratory Medicine

Cited by 36 publications

References 81 publications

Template synthesis of nanomaterials

Template synthesis of nanomaterials

Designing a nano-interface in a microfluidic chip to probe living cells: Challenges and perspectives

An integrated method for mutation detection using on‐chip sample preparation, single‐stranded conformation polymorphism, and heteroduplex analysis

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