Miniaturized Disposable Buckypaper-Polymer Substrate Based Electrochemical Purine Sensing Platform

Abstract

Herein, the construction of a novel, low-cost and disposable electrochemical sensor is presented by using recyclable material as an alternative with bucky paper (BP) as an electrode material. The polyethyleneterephthalate (PET) obtained from a drinking bottle was used as sensing platform. The CO2 laser were used for producing sensing substrate and the desired three electrode pattern of BP. This was attached on the PET substrate using double side tape. The lamination process was used to define the geometric area and for insulating the electrode. The determination of Xanthine (Xn) and Uric Acid (UA) were carried out as a proof of concept with unmodified BP surface. The electrochemical behaviour of Xn and UA were studied using cyclic voltammetry (CV), differential pulse voltammetry (DPV). The anodic peak for Xn and UA were observed at 0.4 V and 0.2 V respectively. The calibration curve for Xn and UA were obtained in the linear range 10 µM -500 µM and 50 µM - 1000 µM respectively. The LOD obtained for Xn and UA was 6 µM and 22.5 µM respectively. Finally, the proposed method was applied for determination of Xn and UA in human serum sample with good selectivity and high sensitivity.

Facile and Sensitive Detection of Nitrogen-Containing Organic Bases with Near Infrared C-Dots Derived Assays

“…Detection of tetracycline using UV-vis absorption-based sensing assay; Figure S12: Detection of butylamine using PL-based sensing assay; Figure S13: Detection of spermine using PL-based sensing assay; Figure S14: Detection of aniline using PL-based sensing assay; Figure S15: Detection of pyrrole using PL-based sensing assay; Figure S16: Detection of imidazole using PL-based sensing assay; Figure S17: Detection of purine using PL-based sensing assay; Figure S18: Detection of quinoline using PL-based sensing assay; Figure S19: Detection of indole using PL-based sensing assay; Figure S20: Detection of tetracycline using PL-based sensing assay; Table S1: Summary of differences between calculated values and the actual values in percentage (Diff. %) of UV-vis absorption- and PL-based assays for the detection of various NCOBs; Table S2: Comparison of the performance of NCOBs sensing reported in this work to that of prior art [ 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ].…”