Miniaturized bioanalytical systems: enhanced performance through liposomes

Edwards, Katie A.; Bolduc, Olivier R.; Baeumner, Antje J.

doi:10.1016/j.cbpa.2012.05.182

Cited by 37 publications

(21 citation statements)

References 80 publications

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“…The most common transporters belong to functionalized polymers, nanoparticles with conjugated platinum drugs, carbon nanotubes and micelles. Among them, liposomes, which consist of a phospholipid bilayer surrounding an aqueous core, are a very important class of compounds [6]. Liposomes have interesting biological activities including effective drug loading capacity, biocompatibility and improved pharmacokinetics.…”

Section: Introductionmentioning

confidence: 99%

Platinum nanoparticles induce damage to DNA and inhibit DNA replication

et al. 2017

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Sparsely tested group of platinum nanoparticles (PtNPs) may have a comparable effect as complex platinum compounds. The aim of this study was to observe the effect of PtNPs in in vitro amplification of DNA fragment of phage λ, on the bacterial cultures (Staphylococcus aureus), human foreskin fibroblasts and erythrocytes. In vitro synthesized PtNPs were characterized by dynamic light scattering (PtNPs size range 4.8–11.7 nm), zeta potential measurements (-15 mV at pH 7.4), X-ray fluorescence, UV/vis spectrophotometry and atomic absorption spectrometry. The PtNPs inhibited the DNA replication and affected the secondary structure of DNA at higher concentrations, which was confirmed by polymerase chain reaction, DNA sequencing and DNA denaturation experiments. Further, cisplatin (CisPt), as traditional chemotherapy agent, was used in all parallel experiments. Moreover, the encapsulation of PtNPs in liposomes (LipoPtNPs) caused an approximately 2.4x higher of DNA damage in comparison with CisPt, LipoCisPt and PtNPs. The encapsulation of PtNPs in liposomes also increased their antibacterial, cytostatic and cytotoxic effect, which was determined by the method of growth curves on S. aureus and HFF cells. In addition, both the bare and encapsulated PtNPs caused lower oxidative stress (determined by GSH/GSSG ratio) in the human erythrocytes compared to the bare and encapsulated CisPt. CisPt was used in all parallel experiments as traditional chemotherapy agent.

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Section: Introductionmentioning

confidence: 99%

Platinum nanoparticles induce damage to DNA and inhibit DNA replication

et al. 2017

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“…[1][2][3][4][5][6][7][8][9] Liposomes have already found applications in the medical field, acting as carriers for drug and gene delivery, 10,11 and in bioanalytical systems, especially in integrated microfluidic devices. 12,13 We recently reported a new microfluidic method, Octanol-assisted Liposome Assembly (OLA), to produce liposomes in a controlled and efficient manner. 14 There are a number of important advantages of OLA over existing bulk methods (extrusion, 15 hydration, 16 and electroformation 17 ) and microfluidic methods: [18][19][20][21][22][23] (1) The use of a biocompatible organic solvent that enables an efficient and quick de-wetting of the double-emulsion droplets within a few minutes.…”

Section: Introductionmentioning

confidence: 99%

On-chip density-based purification of liposomes

2017

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Due to their cell membrane-mimicking properties, liposomes have served as a versatile research tool in science, from membrane biophysics and drug delivery systems to bottom-up synthetic cells. We recently reported a novel microfluidic method, Octanol-assisted Liposome Assembly (OLA), to form cell-sized, monodisperse, unilamellar liposomes with excellent encapsulation efficiency. Although OLA provides crucial advantages over alternative methods, it suffers from the presence of 1-octanol droplets, an inevitable by-product of the production process. These droplets can adversely affect the system regarding liposome stability, channel clogging, and imaging quality. In this paper, we report a density-based technique to separate the liposomes from droplets, integrated on the same chip. We show that this method can yield highly pure (>95%) liposome samples. We also present data showing that a variety of other separation techniques (based on size or relative permittivity) were unsuccessful. Our density-based separation approach favourably decouples the production and separation module, thus allowing freshly prepared liposomes to be used for downstream on-chip experimentation. This simple separation technique will make OLA a more versatile and widely applicable tool.

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“…Liposomes are applied and utilized in biomedical applications of Drug Delivery System (DDS) and the research is focused on drug encapsulation, targeting, and release control. The idea of releasing liposome contents as a bionsensor component is well known and fluorescent molecules or redox couples are often encapsulated by the liposomes . An ELISA‐inspired approach to biological detection consisting entirely of on‐chip electrical impedance sensing and applied to HIV detection was reported .…”

Section: Electrochemical Impedance Spectroscopy As a Tool For Investimentioning

confidence: 99%

Model Biological Membranes and Possibilities of Application of Electrochemical Impedance Spectroscopy for their Characterization

et al. 2017

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Biological membranes are essential parts of living systems. They represent an interface between intracellular and extracellular space. Depending on their structure, they often perform very complex functions and play an important role in the transport of both charged and uncharged particles in any organism. Structure of the biological membranes, which play very important role in electrochemical processes inside living organisms, is very complicated and still not precisely defined and explained. Model lipid membranes are used to gain detail information about properties of real biological membranes and about associated electrochemical processes. Electrochemistry, especially electrochemical impedance spectroscopy (EIS), can play a useful role in the characterization of properties of model lipid membranes (planar and supported lipid bilayers, tethered lipid membranes, liposomes, etc.). This review is focused on model biological membranes and the possibilities and limitations of electrochemical methods and namely of EIS in this field.

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Miniaturized bioanalytical systems: enhanced performance through liposomes

Cited by 37 publications

References 80 publications

Platinum nanoparticles induce damage to DNA and inhibit DNA replication

Platinum nanoparticles induce damage to DNA and inhibit DNA replication

On-chip density-based purification of liposomes

Model Biological Membranes and Possibilities of Application of Electrochemical Impedance Spectroscopy for their Characterization

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