Miniaturized and multiplexed high-content screening of drug and immune sensitivity in a multichambered microwell chip

Sandström, Niklas; Carannante, Valentina; Olofsson, Karl; Sandoz, Patrick A.; Moussaud-Lamodière, Elisabeth L.; Seashore‐Ludlow, Brinton; Ooijen, Hanna van; Verron, Quentin; Frisk, Thomas; Takai, Madoka; Wiklund, Martin; Östling, Päivi; Önfelt, Björn

doi:10.1016/j.crmeth.2022.100256

Cited by 8 publications

(14 citation statements)

References 66 publications

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“…Our group has developed a scaffold-free microwell chip platform to study the dynamics of NK cells in tumor spheroids ( 154 – 156 ). One of the main advantages of this platform is that all the experimental phases (spheroid formation, treatment and NK cell incubation, analysis) can be performed on the chip, which is compatible with high-resolution and live cell imaging.…”

Section: Methods Of Spheroid Formation Applied To Nk Cell Studiesmentioning

confidence: 99%

In vitro models to study natural killer cell dynamics in the tumor microenvironment

2023

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Immunotherapy is revolutionizing cancer therapy. The rapid development of new immunotherapeutic strategies to treat solid tumors is posing new challenges for preclinical research, demanding novel in vitro methods to test treatments. Such methods should meet specific requirements, such as enabling the evaluation of immune cell responses like cytotoxicity or cytokine release, and infiltration into the tumor microenvironment using cancer models representative of the original disease. They should allow high-throughput and high-content analysis, to evaluate the efficacy of treatments and understand immune-evasion processes to facilitate development of new therapeutic targets. Ideally, they should be suitable for personalized immunotherapy testing, providing information for patient stratification. Consequently, the application of in vitro 3-dimensional (3D) cell culture models, such as tumor spheroids and organoids, is rapidly expanding in the immunotherapeutic field, coupled with the development of novel imaging-based techniques and -omic analysis. In this paper, we review the recent advances in the development of in vitro 3D platforms applied to natural killer (NK) cell-based cancer immunotherapy studies, highlighting the benefits and limitations of the current methods, and discuss new concepts and future directions of the field.

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Section: Methods Of Spheroid Formation Applied To Nk Cell Studiesmentioning

confidence: 99%

In vitro models to study natural killer cell dynamics in the tumor microenvironment

2023

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“…Researchers have innovatively combined droplet microfluidic technology and wet spinning with OrgOCs, allowing for the in situ encapsulation and formation of functional human organoids with features of uniformed 3D structure and high throughput testing 113 - 135 . At present, many commercial culture plates (plastic and vitreous forms) have been widely used for 3D organoid formation and subsequent drug testing 136 - 138 . Many other efforts have also been made by integrating sensing elements into culture platforms for continuous monitoring, screening and analysis of organoids.…”

Section: Snapshot Of Oocs Hos and Orgocsmentioning

confidence: 99%

Human organoids-on-chips for biomedical research and applications

Wang,

Ning,

Zhao

et al. 2024

Theranostics

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Human organoids-on-chips (OrgOCs) are the synergism of human organoids (HOs) technology and microfluidic organs-on-chips (OOCs). OOCs can mimic extrinsic characteristics of organs, such as environmental clues of living tissue, while HOs are more amenable to biological analysis and genetic manipulation. By spatial cooperation, OrgOCs served as 3D organotypic living models allowing them to recapitulate critical tissue-specific properties and forecast human responses and outcomes. It represents a giant leap forward from the regular 2D cell monolayers and animal models in the improved human ecological niche modeling. In recent years, OrgOCs have offered potential promises for clinical studies and advanced the preclinical-to-clinical translation in medical and industrial fields. In this review, we highlight the cutting-edge achievements in OrgOCs, introduce the key features of OrgOCs architectures, and share the revolutionary applications in basic biology, disease modeling, preclinical assay and precision medicine. Furthermore, we discuss how to combine a wide range of disciplines with OrgOCs and accelerate translational applications, as well as the challenges and opportunities of OrgOCs in biomedical research and applications.

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“…In the past few years, organoids-small three-dimensional tissue cultures derived from stem cells-have been found to be promising in identifying cancer treatments, how diseases develop, the communication between organs in homeostasis, and in understanding how the organs collectively respond to environmental stressors. Forming them has traditionally been tedious, but the adoption of acoustic manipulation methods has transformed organoid formation into a much more straightforward process using a well-based approach (Olofsson et al, 2018;Sandström et al, 2022). Also using acoustofluidics in a wellbased approach, Ao et al (2022) have shown via brain organoids that the aging of the human brain may principally be driven by monocytes.…”

Section: Forming Tissues and Organoidsmentioning

confidence: 99%

Acoustofluidics

Friend

2023

Front. Acoust.

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The propagation of acoustic waves in fluids and solids produces fascinating phenomena that have been studied since the late 1700s and through to today, where it is finding broad application in manipulating fluids and particles at the micro to nano-scale. Due to the recent and rapid increase in application frequencies and reduction in the scale of devices to serve this new need, discrepancies between theory and reality have driven new discoveries in physics that are underpinning the burgeoning discipline. While many researchers are continuing to explore the use of acoustic waves in microfluidics, some are exploring vastly smaller scales, to nanofluidics and beyond. Because many of the applications incorporate biological material—organelles, cells, tissue, and organs—substantial effort is also being invested in understanding how ultrasound interacts with these materials. Surprisingly, there is ample evidence that ultrasound can be used to directly drive cellular responses, producing a new research direction beyond the established efforts in patterning and agglomerating cells to produce tissue. We consider all these aspects in this mini-review after a brief introduction to acoustofluidics as an emerging research discipline.

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Miniaturized and multiplexed high-content screening of drug and immune sensitivity in a multichambered microwell chip

Cited by 8 publications

References 66 publications

In vitro models to study natural killer cell dynamics in the tumor microenvironment

In vitro models to study natural killer cell dynamics in the tumor microenvironment

Human organoids-on-chips for biomedical research and applications

Acoustofluidics

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