Miniaturised eight‐channel impedance spectroscopy unit as sensor platform for biosensor applications

Broeders, Jeroen; Duchateau, Stijn; Grinsven, Bart van; Vanaken, Wouter; Peeters, Marloes; Cleij, Thomas J.; Thoelen, Ronald; Wagner, Patrick; Ceuninck, W. De

doi:10.1002/pssa.201001199

Cited by 24 publications

(13 citation statements)

References 27 publications

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“…The DC canceller based on a simple RC high-pass filter which is proposed by Analog Devices and is used in some of exemplary AD5933 IC impedance meters [6,10], is not suitable for operating in a wide range of frequencies. The time constant of the RC filter has to be significantly longer than the longest period of the excitation voltage.…”

Section: Sample Excitationmentioning

confidence: 99%

“…An application of the AD5933 IC in biological diagnostics research has been reported by other authors. The AD5933 IC was used in the cell culture growth monitoring [2,3], single cell measurement [4], blood coagulation detection [5], biosensor applications [6], general bioimpedance measurements [7][8][9][10][11], and was also used in technical object monitoring [12,13]. It should be noted however, that in order to obtain the complete information about electrical properties of a measured object and to use a convenient method of the impedance spectra analysis (equivalent circuit modelling) the impedance has to be measured in a wide range of frequencies [14].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Simple Wide Frequency Range Impedance Meter Based on AD5933 Integrated Circuit

Chabowski¹,

Piasecki²,

Dzierka³

et al. 2015

Metrology and Measurement Systems

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As it contains elements of complete digital impedance meter, the AD5933 integrated circuit is an interesting solution for impedance measurements. However, its use for measurements in a wide range of impedances and frequencies requires an additional digital and analogue circuitry. This paper presents the design and performance of a simple impedance meter based on the AD5933 IC. Apart from the AD5933 IC it consists of a clock generator with a programmable prescaler, a novel DC offset canceller for the excitation signal based on peak detectors and a current to voltage converter with switchable conversion ratios. The authors proposed a simple method for choosing the measurement frequency to minimalize errors resulting from the spectral leakage and distortion caused by a lack of an anti-aliasing filter in the DDS generator. Additionally, a novel method for the AD5933 IC calibration was proposed. It consists in a mathematical compensation of the systematic error occurring in the argument of the value returned from the AD5933 IC as a result. The performance of the whole system is demonstrated in an exemplary measurement.

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Section: Sample Excitationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simple Wide Frequency Range Impedance Meter Based on AD5933 Integrated Circuit

Chabowski¹,

Piasecki²,

Dzierka³

et al. 2015

Metrology and Measurement Systems

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“…Related systems include a miniaturized blood coagulation monitoring device developed in [39] and the biosensor platform described in [40]. These two systems also divide the system clock to access lower frequency range for the impedance measurements.…”

Section: Bioimpedancementioning

confidence: 99%

Improving Performance and Versatility of Systems Based on Single-Frequency DFT Detectors Such as AD5933

Matsiev

2014

Electronics

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Abstract:Turning grand concepts such as the Internet of Things (IoT) and Smart Cities into reality requires the development and deployment of a wide variety of computing devices incorporated into the Internet infrastructure. Unsupervised sensing is the cornerstone capability that these devices must have to perform useful functions, while also having low cost of acquisition and ownership, little energy consumption and a small footprint. Impedimetric sensing systems based on the so-called single-frequency DFT detectors possess many of these desirable attributes and are often introduced in remote monitoring and wearable devices. This study presents new methods of improving performance of such detectors. It demonstrates that the main source of systematic errors is the discontinuous test phasor causing the crosstalk between the in-phase and quadrature outputs and the leakage of the input signal. The study derives expressions for these errors as a function of the number of samples and operating frequency, and provides methods for correction. The proposed methods are applied to the operation of a practical device-a network analyzer integrated circuit AD5933-and discussed in detail. These methods achieve complete elimination of leakage errors and expansion of the low limit of the operation frequency range by nearly two decades without additional hardware.

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“…To prevent these ion releases from occurring, the excitation voltage of the device was set to 50 mV peak to peak by adapting the amplifier stage of the impedance setup. Any unwanted DC voltages present in the measurement range are decoupled by means of capacitors connected to the output of the impedance analyzer [16]. Commercially available devices for impedimetric proliferation measurements operate only at fixed frequencies, typically in the 10 kHz-100 kHz range [20][21][22].…”

Section: +mentioning

confidence: 99%

“…2 Materials and methods 2.1 Impedance spectroscopy Previous research resulted in the development of a miniaturized eight-channel impedance analyzer [16], which is used as a starting point for the proliferation measurements. This eight-channel device is fine-tuned for biosensor measurements and is capable of measuring impedance from 10 Ω to 5 MΩ in a frequency range from 10 Hz to 100 kHz.…”

mentioning

confidence: 99%

Cell proliferation monitoring by multiplexed electrochemical impedance spectroscopy on microwell assays

Duchateau

Broeders

Croux

et al. 2013

Phys. Status Solidi C

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Cell proliferation can be monitored by a wide range of well‐established techniques. Most of the principles used rely on optical, single end‐point methods, often involving the use of absorbent, fluorescent or luminescent compounds. These additives can interfere with the cell growth, thus producing distorted results. Electrochemical impedance spectroscopy provides a solution for this problem and enables continuous monitoring without interference. However equipment for this measurement technique is often bulky, highly expensive and lacks multichannel features. This paper presents a low‐cost, compact hardware platform optimized for proliferation measurements, together with custom software to ease interpretation and physical modelling of data. Performance is demonstrated and measurement parameters are fine‐tuned for three commonly used cell types in proliferation measurements. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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Miniaturised eight‐channel impedance spectroscopy unit as sensor platform for biosensor applications

Cited by 24 publications

References 27 publications

Simple Wide Frequency Range Impedance Meter Based on AD5933 Integrated Circuit

Simple Wide Frequency Range Impedance Meter Based on AD5933 Integrated Circuit

Improving Performance and Versatility of Systems Based on Single-Frequency DFT Detectors Such as AD5933

Cell proliferation monitoring by multiplexed electrochemical impedance spectroscopy on microwell assays

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