Miniaturisation of synthesis and screening in nanotiterplates: the concept of NanoSynTest TM

Schober, Andreas; Schlingloff, Gregor; Gross, Ariane; Henkel, Th.; Albert, Jens; Mayer, Günter; Wurziger, H.; Döring, M.; Tietz, H.

doi:10.1007/s00542-003-0328-5

Cited by 11 publications

(9 citation statements)

References 33 publications

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“…Furthermore, there is evidence that the three dimensional architecture and the interaction of multiple cell types influences cellular adhesion properties, migration processes, gene expression and secretion of cellular factors [30][31][32][33][34][35]. Micro bioreactors may lead to new ways in medicinal chemistry to determine ADME/Tox properties of potential drugs [36][37][38][39]. We aim to develop integrated systems for well- controlled 3D organotypic cell cultivation which can be used for routine applications.…”

Section: Introductionmentioning

confidence: 99%

Applied nano bio systems with microfluidics and biosensors for three‐dimensional cell culture

Schober¹,

Fernekorn

Lübbers

et al. 2011

Materialwissenschaft Werkst

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1Applications of nanotechnology and microtechnology lead to a field which could be called nanosystems integration. The next step is the development of nanobiotechnological systems, which could reflect the hierarchical organization of biological systems that utilise scales and laws of nature on all metric scales. As one example of such systems we present the design and construction of a new class of micro bioreactors. It has been shown that 3D culture systems reveal the in vivo situation much better than the cultivation of 2D one cell type alone. For this purpose we have to construct and arrange fluidic devices and a cell-biological environment in such a way, that living cells can survive in a three-dimensional, organ-like structure, enabled by technical devices. Such organ-like cell structures may lead to new ways in medicinal chemistry for the determination of ADME/Tox (absorption-distribution-metabolism-excretion and toxicity) properties of potential drugs. One part of the construction principle is the integration of sensors, preferable novel AlGaN/GaN nanosensors. The latter nanosensors offer the possibility to estimate reactions of cells attached to the sensor surface non-destructively and label-free. These sensors are highly sensitive and biocompatible to cells. Furthermore, they are transparent to enable microscopic and other optical observations of the cells. In an advanced version the sensor is to be integrated to give a better insight into cellular processes of the cells that are cultivated in such micro bioreactors.Keywords: Nanobiotechnology / micro-nanointegration / nanosensors / AlGaN-sensors / microfluidics / assays / advanced cell culture / Die Aufgabe mikro-und nanochtechnologisch hergestellte Funktionselemente zu ganzen Systemen bzw. zu makroskopischen Systemen zu assemblieren, hat zu einem Gebiet geführt, dass man NanoSystemintegration oder auch Mikro-Nanointegration nennen kann. Im Bereich der Nanobiotechnologie führt diese Entwicklung zu Systemen, die die hierarchische Organisation der biologischen Systeme nachbildet, die auf allen geometrischen Skalen von der Nano-über die Mikro-bis zur Makroebene präsent ist bzw. die dort wirkenden Naturgesetze nutzbar macht. Als ein Beispiel stellen wir eine neue Klasse von Mikrobioreaktoren vor. Es konnte gezeigt werden, dass 3D Zellkultursysteme die in vivo Situation wesentlich besser darstellen als 2 dimensionale Zellkultursysteme. Für diesen Zweck müssen wir mikrofluidische Systeme und eine zellbiologische Umgebung so konstruieren, dass lebende Zellen in einer dreidimensionalen, organartigen Struktur, die durch technische Baugruppen gebildet wird, überleben können. Solche organ-ähnlichen Zellverbände können zu neuen Methoden in der medizinischen Wirkstoffforschung führen, um z. B. ADME/Tox Eigenschaften zu bestimmen. Ein Teil der Konstruktion bezieht sich auf die Integration von Sensoren, vorzugsweise von AlGaN-Nanosensoren. Die letzteren ermöglichen es adhärente Zellen nicht destruktiv bzw. labelfrei zu untersuchen. Diese Sensoren sind hoch sensitiv un...

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Section: Introductionmentioning

confidence: 99%

Applied nano bio systems with microfluidics and biosensors for three‐dimensional cell culture

Schober¹,

Fernekorn

Lübbers

et al. 2011

Materialwissenschaft Werkst

Self Cite

View full text Add to dashboard Cite

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“…A three-component condensation reaction took place if urea or thiourea and appropriate aldehyde building blocks (7) were used under modified Biginelli conditions [48]. The urea or thiourea and appropriate aldehydes were heated in the presence of p-toluene sulfonic acid to 100 8C.…”

Section: Diversity Oriented Synthesis In Microreactor Arraysmentioning

confidence: 99%

“…Therefore, a 1536-well glass-microreactor array module was developed. Immobilized 3-aryl-3-oxo-propan amides (4) were synthesized on polystyrenerink resin (1) by the addition of lithium-enolates (3) to the immobilized 4-nitrophenyl carbamate resin (2) (see Figure 1) [47,48]. The resulting 3-aryl-3-oxo-propan amides (4) were used as core for multiple chemical conversions.…”

Section: Diversity Oriented Synthesis In Microreactor Arraysmentioning

confidence: 99%

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Microreactor Array Assembly, Designed for Diversity Oriented Synthesis Using a Multiple Core Structure Library on Solid Support

Groß

Wurziger

Schlingloff³

et al. 2006

QSAR Comb. Sci.

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The application of spatially encoded principles in solid phase combinatorial synthesis requires no chemical or physical coding strategies. The resulting products are encoded by their position inside the array and their synthesis history. The advantages of microreactor arrays for solid phase synthesis as one of the embodiments in the field of microreaction technology are discussed. Here, we review the reactor design, necessary process steps, and a strategy for the diversity oriented array synthesis. In particular, the glass-made microreactor and its assembly for 1563 parallel solid phase reactions, which can be performed at temperatures up to 120 8C, are described. Bead loading and liquid handling steps were adapted to this array. The production of large libraries demands suitable synthesis protocols and building blocks. The optimization of appropriate synthesis conditions is a time-consuming process. A multiple core structure library approach for the efficient synthesis of diverse heterocyclic libraries is described. The aim of this work was to prove the feasibility of the glass-microreaction array for massive parallel library synthesis.

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“…[13] Unfortunately, the situation in biotechnology is quite different from the computer and the ink-jet industry. In particular, biotechnology deals with varying liquids with different properties.…”

Section: Introductionmentioning

confidence: 99%

Evolution and Operating Experiences with Different Drop‐On‐Demand Systems

Burgold

Weise

Fischer

et al. 2005

Macromol. Rapid Commun.

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Summary: We describe the development of different drop‐on‐demand systems particularly for applications for the liquid handling of biopolymers. Different designs of drop‐on‐demand systems developed by the authors are described. Experiments with these systems show the applicability for pipetting different liquids with different properties. Commercially available systems are also tested. A comparison of the different approaches leads to a discussion of the best fields of application of the different approaches or, alternatively, to the potential further development of the drop‐on‐demand technologies.Principle setup of the print heads.magnified imagePrinciple setup of the print heads.

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Miniaturisation of synthesis and screening in nanotiterplates: the concept of NanoSynTest TM

Cited by 11 publications

References 33 publications

Applied nano bio systems with microfluidics and biosensors for three‐dimensional cell culture

Applied nano bio systems with microfluidics and biosensors for three‐dimensional cell culture

Microreactor Array Assembly, Designed for Diversity Oriented Synthesis Using a Multiple Core Structure Library on Solid Support

Evolution and Operating Experiences with Different Drop‐On‐Demand Systems

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