Miniaturisation and validation of a cell-based assay for screening of Ca2+ channel modulators

Tammela, Päivi; Vuorela, Pia

doi:10.1016/j.jbbm.2004.02.008

Cited by 4 publications

(1 citation statement)

References 26 publications

(32 reference statements)

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“…A rigorous sequence of experiments was initially conducted using the manual assay to optimise biofilm-forming conditions before moving into automated environments, as has been proposed by other authors [19]. Biofilm formation was greatly influenced by the initial bacterial concentration and growing time, and significant biofilm formation was proved to occur after 18 h of incubation in optimal conditions (37 • C, 200 rpm) with an initial bacterial concen- Fig.…”

Section: Discussionmentioning

confidence: 99%

Automating a 96-well microtitre plate model for Staphylococcus aureus biofilms: an approach to screening of natural antimicrobial compounds

Sandberg¹,

Määttänen²,

Peltonen³

et al. 2008

International Journal of Antimicrobial Agents

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Section: Discussionmentioning

confidence: 99%

Automating a 96-well microtitre plate model for Staphylococcus aureus biofilms: an approach to screening of natural antimicrobial compounds

Sandberg¹,

Määttänen²,

Peltonen³

et al. 2008

International Journal of Antimicrobial Agents

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HPLC micro-fractionation coupled to a cell-based assay for automated on-line primary screening of calcium antagonistic components in plant extracts

et al. 2004

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High performance liquid chromatography (HPLC) micro-fractionation was successfully coupled to an automated 45Ca2+ uptake assay using GH4C1 cells for the separation of natural product extracts and for the primary detection of their calcium antagonistic components. The reliability of the procedure was first established with a reference solution consisting of pure compounds with a known effect on the Ca2+ uptake. No loss of activity was observed to occur after HPLC micro-fractionation. Extracts of Peucedanum palustre and Pinus sylvestris, showing high and no inhibition of Ca2+ uptake as total extracts, respectively, were analysed and the inhibitory activity of the P. palustre extract could be traced to two components, identified as columbianadin and isoimperatorin. As expected, no significant inhibition was observed with the micro-fractionated P. sylvestris samples. In summary, the procedure was found to be applicable for primary detection of calcium antagonistic components in complex matrices and to significantly reduce the time previously needed for bioactivity-guided isolation.

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Calcium Entry Channels and Drug Discovery

Li¹,

Dodge²,

Poll³

et al. 2005

Calcium Signaling, Second Edition

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Miniaturisation and validation of a cell-based assay for screening of Ca2+ channel modulators

Cited by 4 publications

References 26 publications

Automating a 96-well microtitre plate model for Staphylococcus aureus biofilms: an approach to screening of natural antimicrobial compounds

Automating a 96-well microtitre plate model for Staphylococcus aureus biofilms: an approach to screening of natural antimicrobial compounds

HPLC micro-fractionation coupled to a cell-based assay for automated on-line primary screening of calcium antagonistic components in plant extracts

Calcium Entry Channels and Drug Discovery

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