Miniature spectral imaging device for wide-field quantitative functional imaging of the morphological landscape of breast tumor margins

Abstract

Abstract. We have developed a portable, breast margin assessment probe leveraging diffuse optical spectroscopy to quantify the morphological landscape of breast tumor margins during breast conserving surgery. The approach presented here leverages a custom-made 16-channel annular photodiode imaging array (arranged in a 4 × 4 grid), a raster-scanning imaging platform with precision pressure control, and compressive sensing with an optimized set of eight wavelengths in the visible spectral range. A scalable Monte-Carlo-based inverse model is used to generate optical property [μ 0 s ðλÞ and μ a ðλÞ] measures for each of the 16 simultaneously captured diffuse reflectance spectra. Subpixel sampling (0.75 mm) is achieved through incremental x , y raster scanning of the imaging probe, providing detailed optical parameter maps of breast margins over a 2 × 2 cm 2 area in ∼9 min. The morphological landscape of a tumor margin is characterized using optical surrogates for the fat to fibroglandular content ratio, which has demonstrated diagnostic utility in delineating tissue subtypes in the breast.

Evaluating feasibility of an automated 3-dimensional scanner using Raman spectroscopy for intraoperative breast margin assessment

“…Optical imaging methods can satisfy these requirements and obtain real-time tissue information in a non-invasive manner. Various studies have applied different optical methods for margin assessment, including the use of elastic scattering spectroscopy (ESS) 18 , diffuse reflectance spectroscopy (DRS) 19 – 21 , optical coherence tomography (OCT) 22 and photoacoustic tomography (PAT) 23 .…”

Multiscale nonlinear microscopy and widefield white light imaging enables rapid histological imaging of surgical specimen margins

“…Diffuse optical techniques such as diffuse reflectance spectroscopy can quantitatively measure tissue optical properties associated with malignancy, but do not enable direct histological assessment of pathology [41]. Extremely rapid molecular identification of breast margin composition has been recently demonstrated using depth-resolved Raman scattering combined with rotational specimen scanning, although the sensitivity of this technique to different pathologies is still under investigation [42].…”

Quantitative assessment of distant recurrence risk in early stage breast cancer using a nonlinear combination of pathological, clinical and imaging variables

“…All subjects were recruited following the same eligibility criteria. For the investigation, we built two instruments that had identical specifications based on performance verification and placed at Duke University and at the University of Wisconsin-Madison [35,42]. Patient variables and OncotypeDx recurrence scores were entered into a REDCap database at Duke University (n = 49) and the University of Wisconsin-Madison (n = 63).…”

“…Diffuse reflectance spectra were collected from excised breast tissue specimens from 106 tumor margins using an optical probe described previously [34,35,42]. We measured two negative margins in all tumor samples except six patients due to intraoperative time constraints.…”

“…In fact, previous studies have shown that tumorigenic signaling and cellular persistence is enhanced with increased collagen matrix stiffness and the nature of fiber matrix alignment within the breast [38][39][40][41]. Our group has previously developed a spectral imaging probe that can intraoperatively capture a measure of local breast density (LBD) for every square millimeter of a given breast tumor margin [35,42]. While a clear association was established between LBD and surgical margin status-and thereby risk of local recurrence [34]-no association with distant recurrence risk has been established.…”