Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

Cooper

2008

J. Lightwave Technol.

Self Cite

DNA detection technology has developed rapidly due to its extensive application in clinical diagnostics, bioengineering, environmental monitoring, and food science areas.Currently developed methods such as surface Plasmon resonance (SPR) methods, fluorescent dye labeled methods and electrochemical methods, usually have the problems of bulky size, high equipment cost and time-consuming algorithms, so limiting their application for in vivo detection. In this work, an intrinsic Fabry-Perot interferometric (IFPI) based DNA sensor is presented with the intrinsic advantages of small size, low cost and corrosion-tolerance. This sensor has experimentally demonstrated its high sensitivity and selectivity.In theory, DNA detection is realized by interrogating the sensor's optical cavity length variation resulting from hybridization event. First, a microgap structure based IFPI sensor is fabricated with simple etching and splicing technology. Subsequently, considering the sugar phosphate backbone of DNA, layer-by-layer electrostatic self-assembly technique is adopted to attach the single strand capture DNA to the sensor endface. When the target DNA strand binds to the single-stranded DNA successfully, the optical cavity length of sensor will be increased. Finally, by demodulating the sensor spectrum, DNA hybridization event can be judged qualitatively.This sensor can realize DNA detection without attached label, which save the experiment expense and time. Also the hybridization detection is finished within a few minutes.This quick response feature makes it more attractive in diagnose application. Since the iii sensitivity and specificity are the most widely used statistics to describe a diagnostic test, so these characteristics are used to evaluate this biosensor. Experimental results demonstrate that this sensor has a sensitivity of 6nmol/ml and can identify a 2 bp mismatch. Since this sensor is optical fiber based, it has robust structure and small size ( 125μm ). If extra etching process is applied to the sensor, the size can be further reduced. This promises the sensor potential application of in-cell detection. Further investigation can be focused on the nanofabrication of this DNA sensor, and this is very meaningful topic not only for diagnostic test but also in many other applications such as food industry, environment monitoring.iv Acknowledgement

“…(STD=0.45nm) is thinner than PSS, which is 4.9nm (STD=0.3nm). This experiment result is consistent with previous experiment results [7,83].…”

Section: Results and Analysissupporting

confidence: 94%

“…This phenomenon can be explained by the release of water molecules from the film and reentry from the air due to environment temperature and humidity [83]. …”

Section: Results and Analysismentioning

confidence: 99%

See 1 more Smart Citation

Microgap Structured Optical Sensor for Fast Label-Free DNA Detection

Cooper

2008

J. Lightwave Technol.

Self Cite

“…This is mainly due to the change in density of the DNA monolayer. 5 In this study, the change in optical thickness is measured by a multicavity Fabry-Pérot ͑FP͒ interferometer formed by a short piece of hollow fiber sandwiched between two pieces of optical fiber, 6 as shown in Fig. 1.…”

mentioning

confidence: 99%

Label-free DNA sequence detection using oligonucleotide functionalized optical fiber

Cooper

Applied Physics Letters

et al. 2006

Self Cite

“…The applications are in acoustic (e.g. hydrophones), rotation (eg., gyroscope), chemical, biological etc [67][68][69] to measure strain, temperature etc. All these schemes are used to split the light wave and then recombine at a photo-detector [70].…”

Section: Inferometric Fiber Sensormentioning

confidence: 99%

Review on Developments in Fiber Optical Sensors and Applications

Annamdas¹

2012

IJME

The last couple of decades had witnessed a steep rise in extensive research of optoelectronic and fiber optical communication fields, which resulted in applications focused initially in military, aerospace equipments, and later in health monitoring for medicine, heritage culture and various engineering fields. Health monitoring has attracted the effort of many engineers throughout the world and is fast emerging as a pioneering field. It is helping to improve world economy in two ways; first, directly by providing longevity to all the important structures and secondly, by prevention of untimely failure leading to loss of life and money. This field is very vast, consisting of traditional monitoring methods as well as smart system based methods. The fiber optics belong to finest class of smart materials, there are many types and classifications of fiber optics based on necessity, manufacturer and the end user. This paper, gives a complete over view of fiber sensing systems and their usefulness. In addition, it highlights some of the general fiber optics available in the market with selected examples.