Mini-DNA barcoding for the identification of commercial fish sold in the markets along the Taiwan Strait

Yang

Journal of Food Science

et al. 2022

In this study, DNA barcoding was applied to identify the distinct species of fish products in Guangzhou supermarkets and sushi restaurants in order to confirm whether products were correctly labeled. Samples were analyzed using mitochondrial cytochrome C oxidase subunit I (CO I) gene as the target. Our results showed that the CO I gene of all 139 samples examined was successfully amplified by PCR. When sequenced, 30 samples (21.58%) were mislabeled as the wrong species, 11 samples had insufficient information provided on the label to determine if the labeling was correct (7.91%), and four samples failed sequencing (2.88%). We also found that the use of proper labels for fish products in sushi restaurants was higher than that in supermarkets. As a simple, rapid, and efficient technology, DNA barcoding can be widely used for species identification of fish products. Our work shows that regulation of the labeling of fish products, as we evaluated in Guangzhou and other markets in China, is needed on a global scale.

Section: Resultsmentioning

confidence: 99%

Detection and identification of marine fish mislabeling in Guangzhou's supermarkets and sushi restaurants using DNA barcoding

Yang

Journal of Food Science

et al. 2022

“…The e cacy of species identi cation based on DNA barcoding is also dependent on both su cient inter-and intraspeci c divergence (Xing et al, 2020b), with larger barcode-related intra-and interspeci c differences in genetic distance contributing to the more successful identi cation of species (Dhar and Ghosh, 2017). In the present study, we used the minimum interspeci c and maximum intraspeci c divergence to de ne the barcode gap and found this more e cient than using the means, with both maximum and mean intraspeci c distance values (1.61% and 0.72%, respectively) being inferior to interspeci c distances (9.79-36.38%, with a mean value of 25.99%), with no overlap.…”

Section: Discussionmentioning

confidence: 99%

“…The SeqMan package of DNAStar software was used to splice the forward and reverse sequences determine for each sample. Specimens were identi ed by comparing the COI sequences thus obtained with those in the NCBI database using dedicated statistical tools, and we adopted a sequence similarity of at least 98% as a threshold to determine the validity species identi cation (Armani et al, 2015;Xing et al, 2020b). Subsequently, the sequences were arranged using ClustalW in MEGA6.0 software.…”

Section: Discussionmentioning

confidence: 99%

“…DNA-based techniques are useful tools that can be used to overcome the problems associated with morphological identi cation (Armani et al, 2015;Zeng et al, 2019). In this regard, DNA barcoding, the sequencing of an approximately 650base pair (bp) region of the cytochrome c oxidase I (COI) gene, has gained widespread acceptance in the scienti c literature as an accurate, sensitive, rapid, and standard method for the identi cation of a diverse range of animal lineages, including sh species (Xing et al, 2018;Hebert et al, 2003;Kenchington et al, 2017;Xing et al, 2020a;Xing et al, 2020b).…”

Section: Introductionmentioning

confidence: 99%

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Species identification of larval fish in Hawaiian waters using DNA barcoding

Xing

Wang

et al. 2021

Preprint

Self Cite

Background: Correct identification of fish larvae is notably difficult, given that the morphological features at this stage of development are often poorly defined. However, obtaining such taxonomic information is of considerable importance from the perspectives of fisheries management and conservation. Results: In this study, we collected a total of 95 specimens of larval fish from Hawaiian waters, which we sought to identify using DNA barcoding based on mitochondrial DNA cytochrome oxidase subunit I sequences. Among these, 40 and 52 samples were accordingly identified to the species and genus levels, respectively. The determined average Kimura-2-parameter distances within species, genera, families, and orders were 0.72%, 25.99%, 26.30%, and 27.50%, respectively, and the mean interspecific distance was found to be 36-fold higher than the mean intraspecific distance. Conclusions: The results of this study provide convincing evidence that DNA barcoding can serve as an effective tool for the accurate species identification of larval fish in Hawaiian waters, and could thereby make a valuable contribution to species diversity surveys. The findings of this study can make a valuable contribution to our knowledge of the diversity and dispersal of larval fish in Hawaiian waters.

“…The scarcity of this species that is mostly appreciated by consumers has led to an increase in their value, commanding a higher market price [29,30]. The high price and limited supply of yellow croaker have resulted in increases in the vulnerability of threat to its populations and the occurrence of fraudulent substitution and mislabeling [31].…”

Section: Identification Of Incorrectly Labeled Fish Sample For Substitution Detectionmentioning

confidence: 99%

Rapid Identification between Two Fish Species Using UV-Vis Spectroscopy for Substitution Detection

Chai

Wang

2021

Molecules

Fish species substitution and fraud has become a worldwide economic issue in the seafood industry. In this study, an ultraviolet-visible (UV-Vis) spectroscopy-based method was developed for the identification of fish samples. Sixty fish samples from twelve commonly consumed fish species in China were analyzed as models to testify the protocol. The obtained results show that UV-Vis spectroscopy combined with chemometric analysis, such as principal component analysis (PCA), can accurately distinguish two fish species by boiling fish tissue sample in trifluoroacetic acid (TFA) solution for 2 min and analyzing the resultant samples using a UV-Vis spectrometer. The developed strategy was successfully applied to the classification and identification of fish samples on the market. It is a promising strategy that can be applied to the classification and authenticity testing of closely related fish species in order to detect and recognize fish substitution.