Mini-Chromosome Maintenance Protein Family: Novel Proliferative Markers - The Pathophysiologic Role and Clinical Application

Karimi, Shirin; Sadr, Makan

doi:10.5772/18162

Cited by 1 publication

(1 citation statement)

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“…12 Similar classes of proteins have been found in murine, xenopus, and human cells with significant maintenance of gene sequences. 12,16 Human MCM2 (BM28) was first recognized as a nuclear protein. 17 The MCM2 protein is the part of the family of six MCM (2 to 7) proteins with similar molecular structure and biochemical functions and are equally important for continuous DNA replication.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Expression of MCM-2 for Evaluation of Proliferative Activity in the Epithelial Lining of Radicular Cyst, Dentigerous Cyst, and Odontogenic Keratocyst: A Retrospective Study

Swain¹,

Patel²,

Pathak³

et al. 2018

Journal of Contemporary Dentistry

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Aim:To evaluate and compare immunohistochemical expression of minichromosome maintenance protein-2 (MCM-2) for proliferative activity in the epithelial lining of radicular cyst (RC), dentigerous cyst (DC) and odontogenic keratocyst (OKC) Materials and methods: This retrospective study was conducted on 45 formalin-fixed paraffin-embedded tissue blocks of odontogenic cysts, which were retrieved from the archives. Sections from RC, DC, and OKC were subjected to MCM-2 nuclear staining technique. For each of the specimens, intensity and extent of MCM-2 expressions were evaluated by a comprehensive scoring formula; 100 nuclei were assessed in the epithelial lining of each specimen, under 400× magnification. Scoring was done for positively stained nuclei and expressed as percentage. Results:The mean (%) MCM-2 immunopositive nuclei in the epithelial lining were higher in OKC cases (10.93%) as compared with RC (10.40%) and DC (3.07%), and the difference was statistically significant between the OKC and DC (p = 0.046). The mean (%) MCM-2 expression in different histopathological grades of inflammation in RC showed a statistically significant difference of MCM-2 expression (%) between cases of mild (n = 4) and severe (n = 2) degrees of inflammation (p = 0.012) and also between cases of moderate (n = 9) and severe (2) degrees of inflammation (p = 0.008). The mean (%) MCM-2 expression in inflamed (n = 8) and noninflamed (n = 4) cases of OKC showed a statistically significant difference. The mean (%) MCM-2 expression in cases of DC with (n = 7) and without (n = 7) inflammation also showed a statistically significant difference (p = 0.033). Conclusion:The mean (%) MCM-2 expression was higher in OKC cases as compared with RC and DC, which shows that the epithelium of OKC has a higher proliferative capacity than RC and DC. In present samples, the MCM-2 expression in epithelial lining increased, with increasing grades of inflammation, thus supporting the carcinogenic role of inflammation.

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Section: Discussionmentioning

confidence: 99%