Flotation constitutes a gravity separation process, which originated from the minerals processing field. However, it has, nowadays, found several other applications, as for example in the wastewater treatment field. Concerning the necessary bubble generation method, typically dispersed-air or dissolved-air flotation was mainly used. Various types of biological materials were tested and floated efficiently, such as bacteria, fungi, yeasts, activated sludge, grape stalks, etc. Innovative processes have been studied in our Laboratory, particularly for metal ions removal, involving the initial abstraction of heavy metal ions onto a sorbent (including a biosorbent): in the first, the application of a flotation stage followed for the efficient downstream separation of metal-laden particles. The ability of microorganisms to remove metal ions from dilute aqueous solutions (as most wastewaters are) is a well-known property. The second separation process, also applied effectively, was a new hybrid cell of microfiltration combined with flotation. Sustainability in this field and its significance for the chemical and process industry is commented.