Mineralized Tissue Formation by BMP2-transfected Pulp Stem                Cells

Yang, Xu; Kraan, P.M. van der; Bian, Zhuan; Fan, Mingwen; Walboomers, X. Frank; Jansen, John A.

doi:10.1177/0022034509346258

Cited by 82 publications

(62 citation statements)

References 28 publications

(39 reference statements)

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“…An ectopic mouse model was used to evaluate the in vivo activity of ST Mi/GH 26,27) . Under isoflurane anesthesia, the backs of twelve 10-week-old BALB/c immunocompromised nude mice (SHIMIZU Laboratory Supplies, Kyoto, Japan) were washed, and disinfected.…”

Section: In Vivo Evaluation Of Odontoblastic Differentiation By St Mi/ghmentioning

confidence: 99%

Controlled release of simvastatin from biodegradable hydrogels promotes odontoblastic differentiation

Miyazawa

Matsuno

Asano

et al. 2015

Dental Materials Journal

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The objective of this study was to investigate the odontoblastic differentiation of dental pulp stem cells (DPSC) by biodegradable hydrogels incorporating simvastatin micelles, both in vitro and in vivo. Simvastatin (ST) was incorporated into the micelles of gelatin grafted with L-lactic acid oligomers (LAo) to allow water-solubilization. The simvastatin-LAo-grafted gelatin (LAo-g-gelatin) micelles were mixed with gelatin, followed by chemical crosslinking to form gelatin hydrogels (ST Mi/GH). The ST Mi were released from the gelatin hydrogel granules (GH) through enzymatic degradation. The ST Mi enhanced alkaline phosphatase activity, calcium deposition, and bone morphogenic protein-2 secretion of DPSC. When implanted subcutaneously into mice, the ST Mi/GH treated group exhibited increased dentin sialoprotein and calcium deposition, compared with those treated with GH plus free ST. It is possible to achieve odontoblastic differentiation of DPSC through the controlled release of ST from GH.

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Section: In Vivo Evaluation Of Odontoblastic Differentiation By St Mi/ghmentioning

confidence: 99%

Controlled release of simvastatin from biodegradable hydrogels promotes odontoblastic differentiation

Miyazawa

Matsuno

Asano

et al. 2015

Dental Materials Journal

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“…DPSCs were placed into 96-well plates at a density of 2x10 4 cells per well and maintained overnight. The DPSCs were then treated with medicated media at various concentrations of the total flavonoid from Drynaria fortunei (0.01, 0.05 and 0.1 g/l) in a volume of 100 µl.…”

Section: Dpsc Proliferation Analysis Using Colorimetric 3-(4 5-dimetmentioning

confidence: 99%

“…Gronthos et al were the first to identify and isolate dental pulp stem cells (DPSCs) (2), and Grottkau et al demonstrated that DPSCs were undifferentiated mesenchymal stem cells in tooth pulp with self-renewal, regulated cell proliferation and the potential to transdifferentiate into various types of specialized tissue cells including osteoblast, adipocyte, neurocyte, chondrocyte and smooth muscle cells (3). Findings of recent studies suggested that DPSCs have the ability to differentiate into the bone cell phenotype in vitro and form mineralized tissue in vivo (4,5). These results suggest that DPSCs are likely have a positive impact on periodontal tissue engineering and bone regeneration.…”

Section: Introductionmentioning

confidence: 99%

Effects of total flavonoids from Drynaria fortunei on the proliferation and osteogenic differentiation of rat dental pulp stem cells

Huang

Yuan

Yang

2012

Molecular Medicine Reports

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Abstract. Dental pulp stem cells (DPSCs) have the potential to form bone, nerve and fat, and are a candidate for use in regenerative medicine. Previous studies indicated that total flavonoids from Drynaria fortunei show a stimulative effect on the proliferation and osteogenic differentiation of osteoblastic MC3T3-E1 cells in vitro. This study aimed to investigate the effect of total flavonoids from Drynaria fortunei on the proliferation and osteogenic differentiation of rat DPSCs, and to further clarify the mechanisms involved. DPSCs were isolated by enzymatic digestion and identified using the CD44, CD29 and CD34 markers by immunohistochemistry, and exposed to 0.01, 0.05 and 0.1 g/l total flavonoids from Drynaria fortunei media. Total flavonoids from Drynaria fortunei promoted the proliferation of DPSCs in a dose-dependent manner and this effect may depend on the shortening of the G0/G1 phase and promotion of the S phase. Compared with the control group, the levels of alkaline phosphatase (ALP) and the expression of osteogenic genes increased with the concentrations of total flavonoids from Drynaria fortunei, and the volume and number of calcified nodules in the Drynaria groups was bigger compared to the control group. These results suggest that total flavonoid from Drynaria fortunei directly stimulates DPSC proliferation and osteogenic differentiation, and may serve as a new promising candidate drug for dental tissue engineering and bone regeneration.

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“…In the oral and maxillofacial area, there is a growing need to regenerate alveolar bone to treat periodontitis and for implant dentistry, and cell-based therapies are receiving increasing attention. 1,7,8,10,18,32 For dentists or oral surgeons, dental pulps and periodontal ligaments from discarded teeth, such as third molars, baby teeth that have fallen out, and premolars removed before orthodontic treatment are readily accessible and require an easy and minimally invasive procedure to obtain and store these tissues for future use. 9,11,21,23 Furthermore, banking a patient's own dental tissues is a reasonable and simple alternative to harvesting MSCs from other tissues.…”

Section: Discussionmentioning

confidence: 99%

Engineering Bone Formation from Human Dental Pulp- and Periodontal Ligament-Derived Cells

Sumita

Ikeda

et al. 2010

Ann Biomed Eng

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Abstract-A robust method for inducing bone formation from cultured dental mesenchymal cells has not been established. In the present study, an efficient method for generating bone tissue from cultured human dental pulp-and periodontal ligament-derived cells (DPCs and PDLCs, respectively) was designed using exogenous bone morphogenetic protein 2 (BMP2). DPCs and PDLCs showed enhanced alkaline phosphatase (ALP) activity and calcified nodule formation in medium containing dexamethasone, -glycerophosphate, and ascorbic acid (osteogenic medium). However, the addition of recombinant human (rh) BMP2 to osteogenic medium remarkably increased ALP activity and in vitro calcification above the increases observed with osteogenic medium alone. rhBMP2 also significantly upregulated the expression of osteocalcin, osteopontin, and dentin matrix protein 1 mRNA in both cell types cultured in osteogenic medium. Finally, we detected prominent bone-like tissue formation in vivo when cells had been exposed to rhBMP2 in osteogenic medium. In contrast, treatments with osteogenic medium or rhBMP2 alone could not induce abundant mineralized tissue formation. We propose here that treatment with rhBMP2 in osteogenic medium can make dental mesenchymal tissues a highly useful source of cells for bone tissue engineering. In addition, both DPCs and PDLCs showed similar and remarkable osteo-inducibility.

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Mineralized Tissue Formation by BMP2-transfected Pulp Stem Cells

Cited by 82 publications

References 28 publications

Controlled release of simvastatin from biodegradable hydrogels promotes odontoblastic differentiation

Controlled release of simvastatin from biodegradable hydrogels promotes odontoblastic differentiation

Effects of total flavonoids from Drynaria fortunei on the proliferation and osteogenic differentiation of rat dental pulp stem cells

Engineering Bone Formation from Human Dental Pulp- and Periodontal Ligament-Derived Cells

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