Mineralization of invertebrate cartilage

Eilberg, Ralph G.; Zuckerberg, D. A.; Person, Philip

doi:10.1007/bf02563993

Cited by 20 publications

(9 citation statements)

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“…Furthermore, the mode of calcium accumulation differed dramatically in the larva as it was observed to be entirely cellular rather than within the extracellular matrix. This cellular uptake of calcium mineral demonstrated by the larval cartilage, when examined at the light microscopic level, is similar to the in vitro mineralization of invertebrate cartilages observed by Eilberg et al (1975) and Libbin et al (1976). However, the invertebrate cartilages did not mineralize at all at 20°C but only at higher temperatures which the organisms do not normally encounter and which was given as a reason for the absence of invertebrates possessing mineralized cartilage in nature.…”

Section: Discussionsupporting

confidence: 77%

“…Control cartilages were heat-killed by boiling for 5 min before being placed in the flasks. The culture media, a hydroxyapatite metastable solution (Eilberg et al 1975) was made up as follows. In a 1 litre volumetric flask, a 100 ml aqueous salt solution (NaHCO,, 220 mM I F ' ; KCI, 50 mM I -I ; NaCI, 702 mM I -I ) was added to 50 ml of an aqueous phosphate buffer (NaH,PO,, 25.1 mM I-'; NaH,PO,, 6.45 mM I-' as NaH, PO,.H,O) and 200 ml of distilled-deionized water (DDH,O).…”

Section: Culture Of Cartilage In Media Metastable For Hy Droxyapatitementioning

confidence: 99%

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Calcification of Cartilage from the Lamprey Petromyzon marinus (L.) in vitro

Langille

Hall

1993

Acta Zoologica

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Adult and larval lamprey cartilage, normally unmineralized, was cultured in a medium meta‐stable for hydroxyapatite for periods of up to 12 days at 20, 30 or 37°C. Histochemical analysis revealed a temperature‐dependent increase over time in calcium phosphate incorporation into the extracellular matrix (ECM) of adult cartilage concomitant with the incorporation of calcium as detected by gravimetric and radioisotopic methods. Ultrastructural analysis revealed the presence of ECM dense bodies (40 nm average) containing crystalline material which increased in number with increased mineral incorporation but were absent from control cartilage. Additionally, electron‐dense particles (15–20 nm) found in close association with the ECM fibrils in some regions of mineralized cartilage may represent sites of amorphous calcium phosphate. Larval cartilage incorporated much less calcium, less uniformly over time than did adult cartilage. Histochemical and ultrastructural analysis revealed that calcium had accumulated in the chondrocytes instead of the ECM and this proved detrimental, leading to the death of the cells. The discovery that adult lamprey cartilage calcifies in vitro under appropriate conditions, suggests that petromyzonids, or their direct agnathan ancestors, may have possessed mineralized skeletons and that this ability is ‘repressed’ in extant lampreys owing primarily to the environment they inhabit.

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Section: Discussionsupporting

confidence: 77%

Section: Culture Of Cartilage In Media Metastable For Hy Droxyapatitementioning

confidence: 99%

Calcification of Cartilage from the Lamprey Petromyzon marinus (L.) in vitro

Langille

Hall

1993

Acta Zoologica

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“…Cartilage has been present in vertebrates since their origin, somewhat more than a half-billion years ago (0rvig, 1951Moss, 1964aMoss, , 1968a, and it may have an ancestral invertebrate heritage (Person and Philpot, 1963;Eilberg et al, 1975;Person, Chapter 2 in this volume; see also Hyman, 1940).…”

Section: Antiquity Of Cartilage and Bonementioning

confidence: 96%

Vertebrate Cartilages

Moss¹,

Moss-Salentijn²

1983

Cartilage

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“…Nonstoichiometric hydroxyapatite (with a Ca/P molar ratio unequal to 1.67) is one of the major inorganic components of vertebrate bone and teeth [1][2]. In elevated temperatures HAp can also be mineralized from marine invertebrate cartilage tissues [3]. Synthetic hydroxyapatite (with the Ca/P molar ratio of 1.67) can be prepared by various methods, such as wet precipitation, electrophoresis, hydrothermal treatment and a simple sol-gel technique.…”

Section: Introductionmentioning

confidence: 99%

Nano-particle doped hydroxyapatite material evaluation using spectroscopic polarization sensitive optical coherence tomography

Strąkowska

Trojanowski

Gardas³

et al. 2015

SPIE Proceedings

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Bio-ceramics such as hydroxyapatite (HAp) are widely used materials in medical applications, especially as an interface between implants and living tissues. There are many ways of creating structures from HAp like electrochemical assisted deposition, biomimetic, electrophoresis, pulsed laser deposition or sol-gel processing. Our research is based on analyzing the parameters of the sol-gel method for creating thin layers of HAp. In order to achieve this, we propose to use Optical Coherence Tomography (OCT) for non-destructive and non-invasive evaluation. Our system works in the IR spectrum range, which is helpful due to the wide range of nanocomposites being opaque in the VIS range. In order to use our method we need to measure two samples, one which is a reference HAp solution and second: a similar HAp solution with nanoparticles introduced inside. We use silver nanoparticles below 300 nm. The aim of this research is to analyze the concentration and dispersion of nanodopants in the bio-ceramic matrix. Furthermore, the quality of the HAp coating and deposition process repetition have been monitored. For this purpose the polarization sensitive OCT with additional spectroscopic analysis is being investigated. Despite the other methods, which are suitable for nanocomposite materials evaluation, the OCT with additional features seems to be one of the few which belong to the NDE/NDT group. Here we are presenting the OCT system for evaluation of the HAp with nano-particles, as well as HAp manufacturing process. A brief discussion on the usefulness of OCT for bio-ceramics materials examination is also being presented.

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Mineralization of invertebrate cartilage

Cited by 20 publications

References 5 publications

Calcification of Cartilage from the Lamprey Petromyzon marinus (L.) in vitro

Calcification of Cartilage from the Lamprey Petromyzon marinus (L.) in vitro

Vertebrate Cartilages

Nano-particle doped hydroxyapatite material evaluation using spectroscopic polarization sensitive optical coherence tomography

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