Mineral weathering in ectomycorrhizosphere of subalpine fir (Abies lasiocarpa (Hook.) Nutt.) as revealed by soil solution composition

Arocena, J. M.; Glowa, K. R.

doi:10.1016/s0378-1127(99)00298-4

Cited by 67 publications

(33 citation statements)

References 22 publications

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“…Ca-oxalates) through fungally mediated mineralization processes (Garvie, 2003). The formation of oxalates contributes to the Ca, Mg and C recycling in nature and to bio-weathering of rock substrates (Ascaso et al, 1998;Arocena et al, 2000;Sterflinger, 2000). Frost et al (2003) considered the finding of organic oxalates (e.g.…”

Section: Introductionmentioning

confidence: 99%

In vitro formation of Ca-oxalates and the mineral glushinskite by fungal interaction with carbonate substrates and seawater

Kolo

Claeys

2005

Biogeosciences

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Abstract. This study investigates the in vitro formation of Ca-oxalates and glushinskite through fungal interaction with carbonate substrates and seawater as a process of biologically induced metal recycling and neo-mineral formation. The study also emphasizes the role of the substrates as metal donors. In the first experiment, thin sections prepared from dolomitic rock samples of Terwagne Formation (Carboniferous, Viséan, northern France) served as substrates. The thin sections placed in Petri dishes were exposed to fungi grown from naturally existing airborne spores. In the second experiment, fungal growth and mineral formation was monitored using only standard seawater (SSW) as a substrate. Fungal growth media consisted of a high protein/carbohydrates and sugar diet with demineralized water for irrigation. Fungal growth process reached completion under uncontrolled laboratory conditions. The newly formed minerals and textural changes caused by fungal attack on the carbonate substrates were investigated using light and scanning electron microscopy (SEM-EDX), x-ray diffraction (XRD) and Raman spectroscopy. The fungal interaction and attack on the dolomitic and seawater substrates resulted in the formation of Ca-oxalates (weddellite CaC 2 O 4 ·2(H 2 O), whewellite (CaC 2 O 4 ·(H 2 O)) and glushinskite MgC 2 O 4 ·2(H 2 O) associated with the destruction of the original hard substrates and their replacement by the new minerals. Both of Ca and Mg were mobilized from the experimental substrates by fungi. This metal mobilization involved a recycling of substrate metals into newly formed minerals. The biochemical and diagenetic results of the interaction strongly marked the attacked substrates with a biological fingerprint. Such fingerprints are biomarkers of primitive life. The formation of glushinskite is of specific importance that is related, besides its importance as a biomineral bearing a recycled Mg, to the possibility of its transformation through diagenetic pathway Correspondence to: K. Kolo (kakolo@vub.ac.be) into an Mg carbonate. This work is the first report on the in vitro formation of the mineral glushinskite through fungal interaction with carbonate and seawater substrates. Besides recording the detailed Raman signature of various crystal habits of Mg-and Ca-oxalates, the Raman spectroscopy proved two new crystal habits for glushinskite. The results of this work document the role of microorganisms as metal recyclers in biomineralization, neo-mineral formation, sediment diagenesis, bioweathering and in the production of mineral and diagenetic biomarkers. They also reveal the capacity of living fungi to interact with liquid substrates and precipitate new minerals.

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Section: Introductionmentioning

confidence: 99%

In vitro formation of Ca-oxalates and the mineral glushinskite by fungal interaction with carbonate substrates and seawater

Kolo

Claeys

2005

Biogeosciences

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“…Fungi are involved in the weathering and transformation of rocks and minerals [5], and the contribution is significant [9]. Ectomycorrhizal fungi involved in weathering K-containing silicate minerals were reported in the laboratory [26,31] and in the field [2]. In liquid-culture experiments, Suillus tomentosus weathers biotite for nutrient uptake [3].…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

“…nidulans strain TN02A7 [24], genetically well characterised, was applied in this study. The modified MMPKRUU medium (gL -1 : 209 salt, 50 ml; trace elements, 1 ml; dextrose, 20 g; riboflavin, 1 ml; uridine, 1.2 g; uracil, 1.1 g; pyridoxine, 1 ml; water up to 1 L. Among which, salt solution (/L): NaNO 3 60 g, NaH 2 O 1.1 g, EDTA 50 g, and a pH that was adjusted to 6.58) was used to culture the strain to study gene differential expression with either a sufficiency or deficiency in K (0.52 g/L KCl or 5.2 g/L K-feldspar powders). A 1 ml aliquot of suspended spore liquid (approximately 2.0 9 10 7 /ml) was inoculated into 100 ml of culture medium.…”

Section: Strain and Culturementioning

confidence: 99%

A Global View of Gene Expression of Aspergillus nidulans on Responding to the Deficiency in Soluble Potassium

2015

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Many researchers have suggested that microbes can accelerate the weathering of silicate minerals. However, many genes and metabolic pathways related to microorganisms obtaining potassium (K) from silicate remain undiscovered. It is feasible to detect the gene expression within the scope of the whole genome through high-throughput sequencing. Surprisingly, only a few reports have shown fungal weathering of silicate using this technology. This study explored differences in gene expression of Aspergillus nidulans, which was cultured with different K sources, KCl, and K-feldspar. A. nidulans RNA was extracted by the construction of a cDNA library. Identification of K-acquisition-related genes with GO and KEGG pathway analysis revealed that primarily differentially expressed genes were related to the biosynthesis of amino acids. When these genes were grouped in accordance with the physiological functions, the genes involved in the synthesis of protease, ribosome, and mitochondria, trans-membrane transport, and oxidative phosphorylation were significantly different. Moreover, 20 genes selected were further tested using RT-qPCR. One half (10 genes) exhibited differential expression, which was consistent with the results of RNA-seq. Combining the results of RNA-seq and RT-qPCR, we summarised a possible way to obtain mineral K in A. nidulans as well. The differentially expressed genes and their associated metabolic pathways revealed will improve the understanding of the molecular mechanisms of microbial weathering of silicate minerals.

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“…A parte aérea das plantas inoculadas apresentou significativos ganhos no teor de Mg, alcançando valores de até 698 vezes o do controle, comprovando que a associação simbiótica proporciona ganhos significativos na absorção de Mg, como já relatado em outros trabalhos (Arocena & Glowa, 2000;Jentschke et al, 2000). As plantas inoculadas com os dicários H4111, PT90A e RV82 foram as que se destacaram na utilização de Mg para o crescimento, comparados aos monocários (p < 0,05) (Quadro 4).…”

Section: Produção De Massa Seca E Absorção De Nutrientes Por E Grandisunclassified

“…As plantas inoculadas com os dicários H4111, PT90A e RV82 apresentaram maior eficiência de utilização de K (p < 0,05) (Quadro 4). 2,47 A 0,4 A 3,6 B 1,3 A 7,1 B 11,2 B 53,5 B 0,95 A 9,22 A 3,17 A 17,44 A 27,11 A 131,27 A 6.422 A 661,70 A 1.924,6 A 349,82 na aquisição desses elementos quando associados às raízes, como já relatado por Arocena & Glowa (2000). A maior absorção de Mg correlacionou-se positivamente com a absorção de K. A correlação positiva entre o teor de Ca e o de Cu, possivelmente, justifica-se pelo fato de o Ca proporcionar maior tolerância fisiológica a esse metal (van Tichelen et al, 2001).…”

Section: Produção De Massa Seca E Absorção De Nutrientes Por E Grandisunclassified

Formação de ectomicorrizas por monocários e dicários de Pisolithus sp. e interações nutricionais em Eucalyptus grandis

Silva

Costa²,

Rocha³

et al. 2007

Rev. Bras. Ciênc. Solo

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RESUMOA germinação de basidiósporos de Pisolithus spp. dá origem a monocários, caracterizados por possuírem um único núcleo haplóide por célula. No campo, o eucalipto associa-se a micélios dicarióticos de Pisolithus spp., não havendo relatos sobre a capacidade dos monocários em estabelecer a associação ectomicorrízica com a planta hospedeira nessas condições. Embora os monocários de Pisolithus sp. sejam capazes de formar a associação ectomicorrízica in vitro, nada se sabe sobre a capacidade dessas estirpes em promover a absorção de nutrientes e o crescimento do eucalipto. O objetivo deste trabalho foi estudar a formação de ectomicorrizas por isolados monocarióticos e dicarióticos de Pisolithus sp. em Eucalyptus grandis, sob condições de casa de vegetação, bem como investigar as relações entre o estabelecimento da associação e o crescimento e a absorção de P, Ca, Mg, K, Cu e Zn pelas plantas. Caracterizou-se, também, a produção de massa seca micelial e a absorção de nutrientes pelos isolados fúngicos in vitro. Os isolados monocarióticos e dicarióticos testados variaram na produção de massa seca micelial e na capacidade de absorção de nutrientes. Em geral, os monocários apresentaram maiores índices de eficiência de utilização de nutrientes do que os dicários. Todos os isolados monocarióticos e dicarióticos formaram ectomicorrizas típicas quando associados com E. grandis. A presença dos isolados fúngicos monocarióticos associados às raízes laterais resultou em aumento do diâmetro radial das células da epiderme radicular, característico das ectomicorrizas de eucalipto, indicando que os monocários, à semelhança dos dicários, são capazes de produzir reguladores de crescimento. As médias de percentagem de colonização das raízes pelos isolados monocarióticos e dicarióticos variaram de 12 a 76 %. A absorção de Ca, Mg e K foi estimulada de forma expressiva pela presença das ectomicorrizas, com aumentos de até 760 vezes, demonstrando a relevância dessa associação no suprimento desses macronutrientes, especialmente o Ca. Alguns isolados monocarióticos são tão eficientes quanto os dicários na colonização radicular e na absorção de nutrientes. A caracterização dos monocários de Pisolithus sp. é necessária para se estabelecer a seleção e o cruzamento dos isolados com características desejáveis, visando ao

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Mineral weathering in ectomycorrhizosphere of subalpine fir (Abies lasiocarpa (Hook.) Nutt.) as revealed by soil solution composition

Cited by 67 publications

References 22 publications

In vitro formation of Ca-oxalates and the mineral glushinskite by fungal interaction with carbonate substrates and seawater

In vitro formation of Ca-oxalates and the mineral glushinskite by fungal interaction with carbonate substrates and seawater

A Global View of Gene Expression of Aspergillus nidulans on Responding to the Deficiency in Soluble Potassium

Formação de ectomicorrizas por monocários e dicários de Pisolithus sp. e interações nutricionais em Eucalyptus grandis

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