Mineral Strength, Sucrose Level and Mannitol Concentration Effects on Cherry Rootstocks Micropropagation

Effect of glycine betaine, polyvinylpyrrolidone and D-mannitol on micropropagation of Sideritis raeseri Boiss and Heldr. subsp. Raeseri.

“…The fortification of MS culture medium with D-mannitol + IBA adversely affected root number of S. raeseri microcuttings, however, 2.5 g/l D-mannitol stimulated root length (4 mm) and raised rooting percentage (25%) but at levels not acceptable in a commercial level. In CAB-6P cherry rootstock, root number and rooting percentage were maximum in half MS medium supplemented with 5 g/l mannitol and 15 g/l sucrose while root length was greatest with 10 g/l mannitol and 30 g/l sucrose in full MS medium (Sarropoulou et al 2016). In the current study employing S. raeseri microplants, root length was also enhanced due to 2.5 g/l D-mannitol + NAA combination treatment.…”

“…In the current study employing S. raeseri microplants, root length was also enhanced due to 2.5 g/l D-mannitol + NAA combination treatment. In Gisela 6, another cherry rootstock, 20 g/l mannitol + 15 g/l sucrose in full MS medium exhibited the maximum root number and 10 g/l mannitol + 30 g/l sucrose the greatest root length [39]. Between the 2 combination treatments, better results for S. raeseri explants (10.29 roots 1.35 cm long, 70% rooting) were obtained with 2.5 g/l D-mannitol + NAA, thus promoting in vitro rooting stage.…”

“…For instance, shoot length was optimum (30.83 mm) with 3 mg/l IBA (D-mannitol-free). According to Sarropoulou et al [39], in CAB-6P cherry rootstock, the supplementation of the MS medium with 5 g/l mannitol positively influenced shoot length. Although, the combination treatment 2.5 g/l D-mannitol + 0.5 mg/l NAA led to increased shoot multiplication percentage (70%) caused at the same time extensive problems of hyperhydricity and necrosis to the explants, thus its use is not recommended for the in vitro shoot proliferation stage of the species under study.…”