Mineral-Solubilizing Soil Bacteria Permanently Green Rocky Slopes by Enhancing Soil Adhesion to the Surface of Rocky Slopes

Abstract

Rocky slopes are vulnerable to landslides and mudslides, which pose a major threat to human life and property. Research is being conducted to improve the adhesion between soil and minerals by mineral-solubilizing bacteria to manage slopes scientifically and develop novel methods for slope greening. From the soil of Nanjing Mufu Mountain’s weathered rock walls, we isolated various soil mineral-solubilizing soil bacteria. During the soil bacterial solubilization test, we discovered that some soil bacteria could enhance the adherence of soil to minerals; therefore, we selected three soil bacteria (NL-7, NL-8, and NL-11) with higher performance for further investigation. Controlled experiments were used to investigate the effects of soil bacteria on soil characteristics (soil moisture content, soil pH, and soil exchangeable metal content) and soil adhesion to minerals. According to the findings, soil bacteria can improve the soil’s adhesion to minerals, improve the soil’s capacity to hold water, regulate soil pH, and solubilize and release exchangeable calcium, magnesium, sodium, and potassium ions. A structural equation modeling analysis was performed to thoroughly examine the relationship between soil characteristics and soil adherence to minerals. The analysis findings showed that soil moisture had the greatest total and direct positive impact on soil adherence to minerals. The most significant indirect impact of soil pH on soil adhesion to minerals is mainly caused by the exchangeable sodium and magnesium ions. Additionally, soil exchangeable sodium ions can only indirectly affect the adhesion of soil to minerals, which is accomplished by controlling soil exchangeable magnesium ions. Therefore, mineral-solubilizing soil bacteria primarily work by enhancing the soil’s water retention capacity to improve the soil’s adherence to minerals. Our study on the effect of mineral-solubilizing bacteria on the adhesion of soil and minerals demonstrates the significant potential of mineral-solubilizing bacteria in spray seeding greening, which will provide data and theoretical support for the formation, application, and promotion of mineral-solubilizing bacteria greening methods and gradually form a new set of scientific and efficient greening methods with Chinese characteristics.

Mineral solubilizing microorganisms and their combination with plants enhance slope stability by regulating soil aggregate structure

“…Jia et al further investigated the screened microorganisms and found that a variety of MSMs could promote root growth, increase root strength, increase the number of root nodules, and affect the number and species of inter-root microorganisms (Jia et al, 2021) on the nitrogen cycling with extremely significant effects (Li C. et al, 2020;Li et al, 2021a;Li et al, 2021b). In addition, we conducted a series of optimization studies on slope spraying substrates, including water retention agents (Wang L. et al, 2022), comprehensively analyzed and ranked the effects of different treatments, and preliminarily applied the MSM-based APG method (Wang L. et al, 2023). The results showed that APG was an effective and feasible slope greening method, with great application value and broad application prospects.…”

Active permanent greening – a new slope greening technology based on mineral solubilizing microorganisms

“…Four typical strains were selected for patent protection based on the test results. We found that these strains positively affected the release of mineral metal ions, plant and root growth, and photosynthesis of plants ( Wu et al., 2017a ; Wu et al., 2017b; Li et al., 2020 ; Jia et al., 2021 ; Wang et al., 2022 ). However, the comprehensive effects of these factors on soil, plants, and roots have not been considered yet, nor have they been integrated with revegetation construction techniques.…”