Mineral Requirements for Growth and Callus Initiation of Tissue Explants Excised from Mature Coconut Palms (<i>Cocos nucifera</i>) and Cultured <i>in vitro</i>

Eeuwens, C. J.

doi:10.1111/j.1399-3054.1976.tb05022.x

Cited by 195 publications

(107 citation statements)

References 9 publications

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“…16 Embryos were initially cultured on Eeuwens 20,21 medium supplemented with 2 mg/l 2,4-D (2,4-dichlorophenoxy-ESEARCH ARTICLE R acetic acid). Callus initiated from embryos was observed for 8 weeks.…”

Section: Plant Materials and Callus Initiationmentioning

confidence: 99%

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ABSTRACT Mature zygotic embryos of Elaeis guineensis Jacq var tenera were excised and cultured on Eeuwens (1976Eeuwens ( , 1978 medium containing 2 mg/l 2,4-D. Callus was initiated from these embryos within 8 weeks. Embryoids were induced from the primary callus cultured on Murashige and Skoog (1962, MS) medium supplemented with 0.5 mg/l 2,4-D. For embryoid differentiation and plantlet regeneration, two successive media were employed.

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confidence: 99%

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Kanchanapoom¹,

Domyoas²

1999

ScienceAsia

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Mature zygotic embryos of Elaeis guineensis Jacq var tenera were excised and cultured on Eeuwens (1976Eeuwens ( , 1978 medium containing 2 mg/l 2,4-D. Callus was initiated from these embryos within 8 weeks. Embryoids were induced from the primary callus cultured on Murashige and Skoog (1962, MS) medium supplemented with 0.5 mg/l 2,4-D. For embryoid differentiation and plantlet regeneration, two successive media were employed. The first medium was MS-CAP devoid of 2,4-D but containing 0.05% activated charcoal . The second medium was MS-CAP containing 0.1 mg/l 2,4-D and 2.5 mg/l BA. The embryoids were harvested at various time, fixed, sectioned, stained and examined microscopically. The histological origin of embryoids was from single cells in the subepidermis along the surface of callus clumps. Embryoids proceeded in a standard development pattern to the globular-, heart-and finally to the cotyledon stage. Secondary embryoids occurred on the cotyledon of primary embryoids and originated from single, densely staining cells of the epidermis.

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Section: Plant Materials and Callus Initiationmentioning

confidence: 99%

“…

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mentioning

confidence: 99%

Untitled

Kanchanapoom¹,

Domyoas²

1999

ScienceAsia

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“…This observation may have been attributed to the high nitrogen content of MS that may have affected root organogenesis of E. guineensis plantlets. Wan and Schuyler (2004) supported this by reporting that reducing the nitrogen content in the MS medium by halving could alleviate the problem of toxicity, and thus resulting in a high frequency of shoot organogenesis in Pinus pinea L. In addition, the compositions of macro and micro elements in Y3 media has been reported to be more suitable for palm species as compared to White medium, or Murashige and Skoog (1962) and Eeuwens (1976). The plantlet regenerated on Y3 medium showed profuse longer roots when compared with MS, this could be attributed to the content of the medium.…”

Section: Discussionmentioning

confidence: 64%

Effect of carbohydrate source on the in vitro germination of Elaeis guineensis Jacq. zygotic embryos on two basal media

Okafor¹,

Okezie²

2016

Afr. J. Biotechnol.

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In this study, seven carbohydrates, namely, glucose, fructose, galactose, sucrose, maltose, raffinose and starch were screened for the most suitable carbon source for the in vitro culture of oil palm (Elaeis guineensis Jacq. var. tenera) zygotic embryos on the basal media of Murashige and Skoog (MS) (1962) and Eeuwen (Y3) (1976). The embryos were excised aseptically and cultured on both media containing the different carbohydrates. Results obtained showed that while both media supported in vitro plantlet regeneration from the embryo explants, Y3 medium was significantly superior to MS medium (P=0.05) in length of root and shoot of plantlets produced. Sucrose was also significantly (P=0.05) superior to glucose and maltose while starch, raffinose, fructose and galactose had the least value in all growth parameters studied. In addition, more uniform plantlets were produced in Y3 media in which sucrose served as carbon source relative to the other six carbon sources tested. The protocol reported here has potential for speeding up germination process within a short period of time for oil palm.

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“…Even though many well-known basal media, including White [59], Nitsch [60] and Murashige and Skoog [67] have been tried, the Y3 medium [68] is the most beneficial, being now most commonly used for makapuno embryo culture [62,66]. This medium provides a greater quantity of micro-elements for tissue growth, including cobalt, copper and particularly iodine.…”

Section: Embryo Culturementioning

confidence: 99%

Biology, propagation and utilization of elite coconut varieties (makapuno and aromatics)

Nguyen

Bandupriya

Foale

et al. 2016

Plant Physiology and Biochemistry

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Coconut farming is not only a vital agricultural industry for all tropical countries possessing humid coasts and lowlands, but is also a robust income provider for millions of smallholder farmers worldwide. However, due to its longevity, the security of production of this crop suffers significantly from episodes of natural disasters, including cyclone and tsunami, devastating pest and disease outbreaks, while also affected by price competition for the principal products, especially the oil. In order to reduce these pressures, high-value coconut varieties (makapuno and aromatics) have been introduced in some regions, on a limited scale, but with positive outcomes. Even though these two varieties produce fruit with delicious solid or flavoursome liquid endosperm, their distinct biochemical and cellular features unfortunately prevent their in situ germination. In fact, embryo rescue and culture have been developed historically to nurture the embryo under in vitro conditions, enabling effective propagation. In an attempt to provide a comprehensive review featuring these elite coconut varieties, this paper firstly introduces their food values and nutritional qualities, and then discusses the present knowledge of their biology and genetics. Further possibilities for coconut in general are also highlighted, through the use of advanced tissue culture techniques and efficient seedling management for sustainable production of these highly distinct and commercially attractive varieties of coconut.

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Mineral Requirements for Growth and Callus Initiation of Tissue Explants Excised from Mature Coconut Palms (Cocos nucifera) and Cultured in vitro

Cited by 195 publications

References 9 publications

Untitled

Untitled

Effect of carbohydrate source on the in vitro germination of Elaeis guineensis Jacq. zygotic embryos on two basal media

Biology, propagation and utilization of elite coconut varieties (makapuno and aromatics)

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