Mineral maturity and crystallinity index are distinct characteristics of bone mineral

Abstract

The purpose of this study was to test the hypothesis that mineral maturity and crystallinity index are two different characteristics of bone mineral. To this end, Fourier transform infrared microspectroscopy (FTIRM) was used. To test our hypothesis, synthetic apatites and human bone samples were used for the validation of the two parameters using FTIRM. Iliac crest samples from seven human controls and two with skeletal fluorosis were analyzed at the bone structural unit (BSU) level by FTIRM on sections 2-4 mum thick. Mineral maturity and crystallinity index were highly correlated in synthetic apatites but poorly correlated in normal human bone. In skeletal fluorosis, crystallinity index was increased and maturity decreased, supporting the fact of separate measurement of these two parameters. Moreover, results obtained in fluorosis suggested that mineral characteristics can be modified independently of bone remodeling. In conclusion, mineral maturity and crystallinity index are two different parameters measured separately by FTIRM and offering new perspectives to assess bone mineral traits in osteoporosis.

Assessment of an osteoblast-like cell line as a model for human primary osteoblasts using Raman spectroscopy

“…This finding could also be due to poorly organised apatite mineral that occurs during active bone remodelling. The findings reported here are consistent with those from a study by Farlay et al who also found that older bone had a higher crystallinity that new bone tissue [38]. The crystallinity of the bone material reported here is significantly higher when compared to hMSCs which are from the same origin [34].…”

Optimal bone mechanical and material properties require a functional glucagon-like peptide-1 receptor

“…Investigation of the bone mineral, by qBEI and FTIR, revealed that neither the degree of mineralization nor the maturity of the mineral was affected by the Glp1r deletion. However, it is important to bear in mind that due to instrument limitations, we were not capable of investigating mineral crystallinity as proposed by Farlay et al (2010). On the other hand, the maturity of the collagen matrix, determined as the 1660:1690 cm K1 ratio, was significantly reduced.…”

Characterization of Bone and Bone-Based Graft Materials Using FTIR Spectroscopy

“…In fact, the apatite crystals comprise two different environments: a non-apatitic hydrated domain, containing diverse labile and reactive ions, surrounds a relatively inert and more stable apatitic domain ( Fig. 1) (Farlay et al, 2010). In the interface between these domains, labile anions (PO 4 3-, HPO 4 2-and CO 3 2-) and cations (Ca 2+, Mg 2+ ) are easily and reversibly exchangeable.…”

“…During maturation and growth of the mineral, the apatitic domain of the crystal increases whereas the hydrated layer decreases, due to continuous ionic exchange between these domains and the solution bath. Soluble charged groups of proteins (Pr) can also participate in the ionic equilibrium of the non-apatitic domain (adapted from Farlay et al, 2010).…”