Mineral fibre toxicity: expression of retinoblastoma (Rb) and phospho-retinoblastoma (pRb) protein in alveolar epithelial and mesothelial cell lines exposed to fluoro-edenite fibres

Musumeci, Giuseppe; Cardile, Venera; Fenga, Concettina; Caggia, Silvia; Loreto, Carla

doi:10.1007/s10565-011-9183-9

Cited by 19 publications

(14 citation statements)

References 28 publications

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“…The lung specimens were homogenized in a buffer containing T-Per buffer (Thermoscientific, Rockford, IL, USA) and a protease inhibitor cocktail (Roche Diagnostics) in a Teflon-glass homogenizer followed by sonication. Protein concentrations were determined using Bradford's method [23] using bovine serum albumin as a standard. Sample proteins (50 μg) were diluted in sodium dodecyl sulphate protein gel loading solution (Invitrogen, Monza, Italy), boiled for 5 min, separated on 4%-12% Bistris gel (Invitrogen) and electroblotted onto nitrocellulose membranes (Invitrogen).…”

Section: Western Blotmentioning

confidence: 99%

Angiogenesis correlates with macrophage and mast cell infiltration in lung tissue of animals exposed to fluoro-edenite fibers

Musumeci

Loreto

Giunta

et al. 2016

Experimental Cell Research

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Section: Western Blotmentioning

confidence: 99%

Angiogenesis correlates with macrophage and mast cell infiltration in lung tissue of animals exposed to fluoro-edenite fibers

Musumeci

Loreto

Giunta

et al. 2016

Experimental Cell Research

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“…Such an activation may induce specific B-cell clones to proliferate as a consequence of the chronic antigenic stimulation sustained by different infectious agents ( 23 , 24 ). Moreover, the involvement of the tumor microenvironment along with cytokine release may be associated with tumor development in workers exposed to asbestos or to other fibers ( 25 – 27 ).…”

Section: Introductionmentioning

confidence: 99%

Occupational and environmental exposure to pesticides and cytokine pathways in chronic diseases (Review)

Gangemi

Gofita

Costa

et al. 2016

International Journal of Molecular Medicine

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149

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Pesticides can exert numerous effects on human health as a consequence of both environmental and occupational exposures. The available knowledge base suggests that exposure to pesticides may result in detrimental reproductive changes, neurological dysfunction and several chronic disorders, which are defined by slow evolution and long-term duration. Moreover, an ever increasing amount of data have identified an association between exposure to pesticides and the harmful effects on the immune system. The real impact of alterations in humoral cytokine levels on human health, in particular in the case of chronic diseases, is still unclear. To date, studies have suggested that although exposure to pesticides can affect the immune system functionally, the development of immune disorders depends on the dose and duration of exposure to pesticides. However, many of the respective studies exhibit limitations, such as a lack of information on exposure levels, differences in the pesticide administration procedures, difficulty in characterizing a prognostic significance to the weak modifications often observed and the interpretation of obtained results. The main challenge is not just to understand the role of individual pesticides and their combinations, but also to determine the manner and the duration of exposure, as the toxic effects on the immune system cannot be separated from these considerations. There is a clear need for more well-designed and standardized epidemiological and experimental studies to recognize the exact association between exposure levels and toxic effects and to identify useful biomarkers of exposure. This review focuses on and critically discusses the immunotoxicity of pesticides and the impact of cytokine levels on health, focusing on the development of several chronic diseases.

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“…Such chronic inflammation is associated with the production of several cytokines and growth factors that may cause cellular proliferation and apoptosis arrest (9,22). On this matter, it has been shown that p27 is downregulated in mesothelial cells following exposure to FE fibers (23). Notably, p27 is considered a tumor suppressor gene due to its function as a regulator of the cell cycle, and in cancers it is often inactivated (24).…”

Section: Discussionmentioning

confidence: 99%

Fluoro-edenite induces fibulin-3 overexpression in non-malignant human mesothelial cells

et al. 2016

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Exposure to asbestos is associated with the development of mesothelioma. In addition to asbestos, other fibers have been identified as risk factors for malignant and non-malignant diseases of the lungs. Among these, fluoro-edenite (FE) was found in patients from Biancavilla (Sicily, Italy) with pleural and lung disease, suggesting its role for tumor expansion. In this context, the identification of early biomarkers useful for the diagnosis of cancer is mandatory. Fibulin-3 represents an important marker for the diagnosis of mesothelioma. However, it remains to be determined whether it is directly associated with exposure to asbestos-like fibers. In the present study, peripheral blood levels of fibulin-3 from 40 asbestos-exposed workers were compared with those detected in 27 street cleaners from Biancavilla. Intriguingly, the results showed that fibulin-3 levels were higher in the group of street cleaners compared with those of the asbestos-exposed workers, suggesting that these workers used the personal protective equipment according to the current regulations. These data suggest that subjects exposed to FE should be monitored for the risk of mesothelioma. FE and volcanic particulates are probably contained within dust inhaled by street cleaners from Biancavilla during their work activities. Based on these criteria, in this study, such fibers were used to treat mesothelial cells (MeT5A) in order to verify whether fibulin-3 levels are affected by these treatments. The results showed that only treatment with FE was associated with fibulin-3 overexpression at both the transcript and protein levels. It was previously demonstrated that mesothelial cells exhibited low levels of p27 following treatment with FE. Notably, p27 downregulation is associated with stathmin upregulation in cancer, conferring an aggressive phenotype of tumor cells. This observation prompted us to perform a computational evaluation demonstrating the activation of stathmin in lung cancer in patients exposed to asbestos. Overall, it can be speculated that both fibulin-3 and stathmin overexpression may be associated with the malignant transformation of mesothelial cells following exposure to asbestos-like fibers.

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Mineral fibre toxicity: expression of retinoblastoma (Rb) and phospho-retinoblastoma (pRb) protein in alveolar epithelial and mesothelial cell lines exposed to fluoro-edenite fibres

Cited by 19 publications

References 28 publications

Angiogenesis correlates with macrophage and mast cell infiltration in lung tissue of animals exposed to fluoro-edenite fibers

Angiogenesis correlates with macrophage and mast cell infiltration in lung tissue of animals exposed to fluoro-edenite fibers

Occupational and environmental exposure to pesticides and cytokine pathways in chronic diseases (Review)

Fluoro-edenite induces fibulin-3 overexpression in non-malignant human mesothelial cells

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